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Five Ways to Produce siRNAs

knockdown has recently led to the development of predictive algorithms for identifying functional siRNAs. The article Designing a Better siRNA describes an algorithm developed by Ambion's partner, Cenix BioScience. This algorithm results in a high proportion of effective siRNA sequences.


In Vitro Preparation

Method #1: Chemical Synthesis
Although more expensive than any of the other methods, the production of chemically synthesized siRNAs requires almost no effort by the researcher. Ambion, and several other companies, provide high quality, chemically synthesized siRNAs on a custom basis. One of the major benefits of chemical synthesis is the large yield of high purity siRNA. Drawbacks include the price and turnaround times (typically 412 days depending on synthesis and purification options). Because of the relatively high price tag, many researchers find it beneficial to screen siRNA sequences using a less expensive preparation method, such as in vitro transcription, and then have the most effective sequence(s) synthesized chemically. A robust design algorithm that results in a high proportion of effective siRNA sequences is available from Ambion. Improved siRNA design criteria decreases the number of sequences that need to be tested, lowering the costs associated with this option.

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