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Five Ways to Produce siRNAs

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1 day + transcription template preparation time

5+ days + DNA oligo

~ 6 hours + DNA oligo

Hands on time

Little to none*

Moderate

Moderate

High

Moderate

Testing to find optimal siRNA sequence

Required

Required

Not needed

Required

Required

Ability to label siRNA (i.e., for analyzing siRNA uptake or localization by fluorescence microscopy)

Yes

Yes

Yes


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Source:


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Related biology technology :

1. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
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Ethylene glycol-bis(2-aminoethyl ether)-N,N,N',N'-tetraacetic acid...
Kit Components: Anti-PARP LumiGLO Chemiluminescent Substrate Immunoblot Blocking Reagent Pyronin Y Reagent Goat Anti-Rabbit IgG, HRP conjugate Double-It Reagent Film Exposure Folders Autor...
ssDNA from PCR DNA in less than 1 hour. No gels, columns, or slurries. Sequence from high quality ssDNA: clearer reads, longer runs....
... Kit, 100 reactions. Prepare circular DNA ... h.Generate microgram quantities of template DNA ... amplified DNA directly for cycle sequencing ... M13 liquid cultures, colonies, plaques, or ...
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