Navigation Links
Five Ways to Produce siRNAs

uch easier in otherwise hard to transfect cells.

Figure 3. Long Term Silencing of GFP with pSilencer 2.1-U6 hygro. HeLa cells expressing cycle 3 GFP were transfected with pSilencer 2.1-U6 hygro containing an insert encoding an siRNA targeting cycle 3 GFP or pSilencer 2.1-U6 hygro without an siRNA-encoding insert. Following transfection, the cells were selected with hygromycin. Three weeks following selection, the cells were analyzed for GFP expression by fluorescence microscopy. Green: GFP. Blue: DAPI stained nuclei. GFP levels were remarkably reduced (94%) in cells transfected with the GFP siRNA-encoding pSilencer 2.1-U6 hygro siRNA Expression Vector as compared to those transfected with an "empty" siRNA expression vector.


Method #5: siRNA Expression Cassettes
siRNA expression cassettes (SECs) are PCR-derived siRNA expression templates that can be introduced into cells directly -- without first being cloned into a vector. Initially described by Castanotto and colleagues (5), SECs include an RNA pol III promoter, a sequence encoding an siRNA hairpin, and an RNA pol III termination site. In contrast to siRNA expression vectors, which require cloning and sequencing prior to use and thus take 12 weeks to prepare, SECs can be generated by PCR in less than a day. SECs thus provide an excellent screening tool to find the most effective siRNA sequence, or to identify the most effective combination of promoter and siRNA in a given cell line. In fact, SECs provide the perfect complement to siRNA expression vectors. By incorporating restriction sites at their ends, SECs found to effectively elicit
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Five Ways Produce siRNAs

(Date:5/21/2013)... 2013 Emerson Resources Inc. , ... Charles Signorino, the Company’s Chief Executive Officer and Chairman ... 32 years of service. As part of Emerson Resources’ ... has been named as Dr. Signorino’s successor. , ... industry, having made a significant contribution to the field ...
(Date:5/21/2013)... ROCKVILLE, Md. , May 21, 2013  Synthetic Biologics, ... the prevention and treatment of serious infectious diseases, announced today ... at the 2 nd Annual Marcum LLP MicroCap Conference ... City at the Grand Hyatt Hotel. Mr. Riley ... A live webcast of Synthetic Biologics, presentation can be ...
(Date:5/21/2013)... May 21, 2013 Bed bugs have become ... My Cleaning Products, many turn to chemical-based pesticides for help ... health and environmental risks those solutions present, the company advised ... Bug Bully instead. , According to My Cleaning Products, ... they used to be. It shared that the pests have ...
(Date:5/21/2013)... May 21, 2013 NANONEX CORPORATION, ... solutions , today announced the successful delivery and ... system, the NX-M200B, to a major Japanese manufacturing ... a fully-automated, production-ready, advanced nanoimprint lithography tool that ... wafer and substrate sizes and types. Based on ...
Breaking Biology Technology:Emerson Resources Announces CEO Retirement and Appointment of Successor 2Emerson Resources Announces CEO Retirement and Appointment of Successor 3Synthetic Biologics to Present at the 2nd Annual Marcum LLP MicroCap Conference 2New Guide to Kill Bed Bugs Published by My Cleaning Products, Company Enumerates Non-Toxic Means to Clear the Pests 2New Guide to Kill Bed Bugs Published by My Cleaning Products, Company Enumerates Non-Toxic Means to Clear the Pests 3Nanonex Announces the Shipment and Installation of an Innovative Nanoimprint Lithography System for Fully Automated Manufacturing 2
... Funds will extend the National ... Honolulu, HI ... $2.8 million in research funding from the National Cancer Institute (NCI) to continue the National ... years. , , , , ,Since 2002, PHRI has participated in the NLST, a research ...
... Stemedica ... today that it has been granted a license by the State of California,s Food ... Manufacturing License recognizes Stemedica as being compliant with California law and the applicable provisions ... ...
... , ... DeSilva + Phillips, Media Investment Bankers, has ... M&A practice. Formerly CEO of IncumediX and COO/CSO at MediServe Information ... on over 140 transactions, including acquisitions, technology license and partnership deals, ...
Cached Biology Technology:Pacific Health Research Institute Awarded $2.8 Million in Research Funding 2Pacific Health Research Institute Awarded $2.8 Million in Research Funding 3Stemedica Achieves Major Milestone - Company Receives License to Manufacture Clinical Grade Stem Cells 2Stemedica Achieves Major Milestone - Company Receives License to Manufacture Clinical Grade Stem Cells 3DeSilva + Phillips, Mediabankers, names Jim Brennan to Head Healthcare IT M&A Practice 2DeSilva + Phillips, Mediabankers, names Jim Brennan to Head Healthcare IT M&A Practice 3
(Date:5/22/2013)... novel technique that can detect molecular variants in chemical ... of the most important, though time-consuming, processes in analytical ... Nature , post-doctoral researcher David Patterson, Professor of ... Center for Free-Electron Laser Science (CFEL) in Hamburg, Germany ... to identify molecular variants apart, and to determine how ...
(Date:5/22/2013)... of potential environmental and human health effects from ... year has led scientists to recommend stronger government ... lithium-ion (Li-ion) battery materials. That,s the conclusion of ... Environmental Science & Technology . , Oladele A. ... have become mainstays for powering everything from smart ...
(Date:5/22/2013)... screening for prostate cancer could become as easy for ... to UC Irvine research published today in the ... more than a decade of work, UC Irvine chemists ... markers for prostate cancer in urine, meaning that the ... and at dramatically lower cost. The same technology could ...
Breaking Biology News(10 mins):Detecting mirror molecules 2Detecting mirror molecules 3UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3
... Aware, Inc. (Nasdaq: AWRE ), a leading supplier ... call to discuss the company,s operating results for the first ... Eastern Time. CEO Michael Tzannes and CFO Rick Moberg will ... Thomson and can be accessed on the Investor Relations page ...
... Park, Md. -- DNA sequencing is the next frontier ... and affordable, it is increasingly available to small laboratories. ... faster rate than ever before. However, the computing ... still has some catching up to do. Large ...
... We have the will but do we have the technology? ... being held May 13 to 15, 2009 at McMaster University ... climate change and global warming. Among the guest speakers ... of Canada and former Premier of Manitoba. He will ...
Cached Biology News:Aware, Inc. Announces Q1 '09 Earnings Conference Call 2A genomic CluE for cloud computing 2A genomic CluE for cloud computing 3
No description available in Ensembl. Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
12(S)-HETE-d8 is used as an internal standard for the quantification of 12(S)-HETE by stable isotope dilution mass spectrometry....
...
...
Biology Products: