Navigation Links
Five Ways to Produce siRNAs

s that have taken up the plasmid. This can help compensate for low transfection efficiencies in difficult to transfect cells. Recently, several groups including Ambion have begun preparing adenoviral, retroviral, and other viral vectors for siRNA expression (see pSilencer adeno 1.0-CMV). These vectors offer the added advantage that cells can be transduced for gene silencing studies, thus reducing problems associated with inefficient plasmid transfection.

Best for:
Long term and other studies in which antibiotic selection of siRNA containing cells is desired. Retroviruses allow for efficient intergration of the siRNA expression cassette.

Not suitable for:
Screening siRNA sequences (note: screening siRNA sequences is possible, but is time and labor intensive with vectors).

Ambion's Solution: pSilencer siRNA Expression Vectors
pSilencer siRNA Plasmid Expression Vectors feature U6 and H1 pol III promoters, an ampicillin resistance gene; an E. coli origin of replication; and one of three antibiotic selectable markers (neomycin, puromycin, or hygromycin resistance genes). An siRNA template is cloned into the vector resulting in siRNA expression once the plasmid is delivered to cells.

pSilencer-adeno siRNA Expression Vectors are also available, making expression vector delivery m
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Five Ways Produce siRNAs

(Date:12/24/2014)... 23, 2014 SoundConnect’s ... introduce cutting edge communication technology, provide continued education ... This webinar series demonstrates online communication features and ... web conferencing’s most innovative and powerful tools. , ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 According to Ross ... Island Affiliate of ITRA Global, the national office market continues ... recovery. This is evidenced by the third quarter’s surprising ... market. Low energy costs have held inflation down and ...
(Date:12/24/2014)... (PRWEB) December 23, 2014 Nashville Fertility Center ... treasurer for the American Society for Reproductive Medicine (ASRM), a ... reproductive medicine. In his new role as treasurer, Dr. ... will have a voice in furthering the mission of ASRM. ...
(Date:12/24/2014)... December 23, 2014 Pipette.com announces their ... can submit their #HolidayInTheLab pictures for a chance to win ... those struggling to think of a holiday gift for their ... provides the ideal holiday presents. The #HolidayInTheLab Contest encourages ...
Breaking Biology Technology:SoundConnect Launches Leading Edge Business Communications Webinar Series 2SoundConnect Launches Leading Edge Business Communications Webinar Series 3ITRA Global Reports Long Island Is Out of Sync With The National Office Market 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 3Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 3Major Pipette Distributor Pipette.com Announces their Holiday in the Lab Contest 2
... 24 A biotech company in Madison, Wisconsin is ... federal government officials that they have volunteered to give ... of Congress or the incoming administration who has forgotten ... "We realize that government employees in positions of responsibility ...
... VITAS Innovative Hospice Care(R) announced,today that Christopher ... chief,information officer. In his new position, Rieder will ... to support the company,s,strategic direction. , ... of IT experience with global organizations,translating business objectives ...
... Informatics, the industry,s leading eClinical solutions provider and ... PRXL ), today announced that it has ... Award. Frost & Sullivan presented the award ... to excel in the field of clinical technology ...
Cached Biology Technology:Mental Stimulus Package: Government Officials who Forgot to Pay Taxes Will Receive Free Memory-boosting Supplement 2Mental Stimulus Package: Government Officials who Forgot to Pay Taxes Will Receive Free Memory-boosting Supplement 3VITAS Innovative Hospice Care(R) Names Christopher Rieder Senior Vice President and Chief Information Officer 2Perceptive Informatics Receives Frost & Sullivan Product Line Strategy Award 2Perceptive Informatics Receives Frost & Sullivan Product Line Strategy Award 3Perceptive Informatics Receives Frost & Sullivan Product Line Strategy Award 4
(Date:12/17/2014)... , Dec. 16, 2014 Research and Markets ... "Global Chemical Sensor Market 2015-2019" report to ... One major trend upcoming in this market ... applications. Chemical sensors help in recording of patient data ...
(Date:12/11/2014)... Markets , http://photos.prnewswire.com/prnh/20130307/600769 ... addition of the "Biometrics Market in the APAC ... One major trend emerging in this market is advances ... important to upgrade biometric solutions to the latest standard ...
(Date:12/11/2014)... , Dec. 10, 2014  Data Sciences International ... has released a new series of digital telemetry ... toxicology researchers. M series, part of the PhysioTel™ ... the best possible physiologic data when incorporating functional ...
Breaking Biology News(10 mins):Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2New telemetry implants expected to change how large animal toxicology studies are conducted 2
... is the fertilized egg cell. Following numerous cell divisions, ... organs and tissues. The largely unexplained process whereby the ... later develop is an astonishing phenomenon. Scientists from the ... of Tbingen and the University of Wageningen, in cooperation ...
... brain works, using ever more sophisticated technology, it seems ... understanding of human nature. One aspect of human nature ... free will. Do our decisions arise from purely ... illusion created by our brains? If so, what will ...
... Each cusp of our teeth is regulated by genes ... also regulates the differentiation of our other organs and ... the Institute of Biotechnology of the University of Helsinki ... complex structures like teeth and organs. The research takes ...
Cached Biology News:How plants put down roots 2'My brain made me do it' 2The formula for making teeth will soon be found 2
Ultra-sensitive, non-radioactive detection of rat Big Endothelin-1 (Big ET-1)...
Request Info...
Request Info...
Peptide Sequence: ovine COX-1 amino acids 272-282 (LMHYPRGIPPQ) To be used in conjunction with Cayman's COX-1 (ovine) polyclonal antiserum (Catalog No. 160108) to block protein-antibody complex f...
Biology Products: