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Five Ways to Produce siRNAs

s that have taken up the plasmid. This can help compensate for low transfection efficiencies in difficult to transfect cells. Recently, several groups including Ambion have begun preparing adenoviral, retroviral, and other viral vectors for siRNA expression (see pSilencer adeno 1.0-CMV). These vectors offer the added advantage that cells can be transduced for gene silencing studies, thus reducing problems associated with inefficient plasmid transfection.

Best for:
Long term and other studies in which antibiotic selection of siRNA containing cells is desired. Retroviruses allow for efficient intergration of the siRNA expression cassette.

Not suitable for:
Screening siRNA sequences (note: screening siRNA sequences is possible, but is time and labor intensive with vectors).

Ambion's Solution: pSilencer siRNA Expression Vectors
pSilencer siRNA Plasmid Expression Vectors feature U6 and H1 pol III promoters, an ampicillin resistance gene; an E. coli origin of replication; and one of three antibiotic selectable markers (neomycin, puromycin, or hygromycin resistance genes). An siRNA template is cloned into the vector resulting in siRNA expression once the plasmid is delivered to cells.

pSilencer-adeno siRNA Expression Vectors are also available, making expression vector delivery m
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4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
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11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
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