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Five Ways to Produce siRNAs

or when the price of chemical siRNA synthesis is an obstacle

Not suitable for:
Long term studies or studies that require large amounts of a single siRNA sequence

Ambion's Solution: Silencer siRNA Construction Kit
The Silencer siRNA Construction Kit produces transfection-ready siRNA at a fraction of the cost of chemical synthesis. The kit is based on a patent-pending in vitro transcription method and can generate up to 15 purified siRNAs in less than 24 hours. The cost and time saved using the Silencer siRNA Construction Kit enable screening sequences of more genes and more potential targets within the target gene to find the most potent siRNA.

Figure 1. Use of Chemically Synthesized and in Vitro Transcribed siRNAs to Induce Gene Silencing. siRNAs targeting -actin were prepared by chemical synthesis (Ambion) or by in vitro transcription using Ambion's Silencer siRNA Construction Kit. HeLa cells were plated at 30,000 cells per well in a 24 well tissue culture plate containing glass slides. The cells were transfected 24 hours after plating, using 2 l siPORT Lipid (Ambion) according to the manufacturer's protocol, at a final siRNA concentration of 75 nM. Immunofluorescence analysis was performed 96 hr post transfection using mouse anti-human -actin primary antibody and a FITC conjugated anti-mouse IgG secondary antibody. Photographs were taken usi
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