Navigation Links
Fine Tuning Your Data Analysis:,,, Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms

false changes, whereas most of the increasing genes in the brain vs. heart comparison represent real biological differences between the two tissues. More changes (~63 additional genes changing greater than 2 fold) can be detected in the different tissues comparison if gamma1 is moved to a less stringent setting (dashed red line), but some of these changes will be false. The number of additional false changes is estimated by the same RNA comparison (~5 genes over 2 fold). Altering gamma has a similar effect on decreasing genes (data not shown).

II.C. Further fine-tuning of Change calls with gamma H and L settings

Accurate detection of change can, in some cases, depend on the Signal range of the changing transcript. Changes in highly expressed transcripts may register different p-values compared to changes in low expressed transcripts, even if the changes are of comparable Signal LogRatios. MAS 5.0 contains a finer level of tuning for change calling, as shown in Figure 9. Each of the gamma cutoffs can be assigned a range of values (bounded by Low and High limits), from which probe set-specific final gamma cutoffs are calculated. Thus, gamma1 is derived from the gamma1L - gamma1H range, and gamma2 is derived from the gamma2L - gamma2H range. The final gamma cutoffs for a given probe set are calculated using a linear interpolation between the L and H limits, based on the probe sets Signal position over the entire arrays Signal range. The Signals used are those from the Experimental array in the comparison. Thus, as shown in Figure 9, if a probe sets Signal is at 30% of the arrays Signal range, the final gamma cutoffs used to decide the Change call for that probe set are set at 30% of the range of values given by the L-H limits for each. The default setting for the L and H limits is to make them equal (i.e., gamma1L = gamma1H and gamma2L = gamma2H). Therefore, the analysis does not use this fine-tuning unless
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
2. The New Affymetrix GeneChip Scanner 3000
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
5. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
6. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
7. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
8. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
9. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. A New Lambda Vector for Mammalian Expression
Post Your Comments:
(Date:11/23/2014)... Apiscent Labs, a privately held ... ingredients to the global pharmaceutical and flavor & ... website, Apiscent.com. , The Apiscent.com homepage welcomes visitors ... easy navigation, rotating images and content focused on ... , The company’s work in Pharmaceutical ...
(Date:11/22/2014)... London (PRWEB) November 21, 2014 Two new ... explore what comes next for ALS research and how the ... , After the Ice Bucket Challenge: Where Does the ALS ... Tuesday, 2 December 2014, Time: 1:30pm ET, ... Join expert speaker Dr. Merit Cudkowicz, Julianne Dorn Professor of ...
(Date:11/22/2014)... 21, 2014 During his lifetime Richard ... need to surround himself with great people and take ... his friends often marveled at his extraordinarily courageous attitude ... life and -- even with his death impending—that’s how ... disease that would ultimately take his life. , Carrying ...
(Date:11/22/2014)... 21, 2014 Prominent academics, leaders ... gather on December 3rd at Genetic Rx, a ... GeneticRx will take place at the Joseph B. ... will discuss the present and future of genetic ... and gene editing—as well as the treatment of ...
Breaking Biology Technology:DrugDev Webinars in December to Explore What Comes Next for ALS Research and How We Can Make Life Easier for Clinical Sites 2Sherry Sharp, Wife of CarMax Founder, Richard Sharp, Accepts Seat on Board of Cure Alzheimer’s Fund 2Sherry Sharp, Wife of CarMax Founder, Richard Sharp, Accepts Seat on Board of Cure Alzheimer’s Fund 3Pioneering Academics and Key Industry Leaders to Discuss Rare Diseases and the Emergence of New Genetic Medicines at Genetic Rx on December 3rd 2
... the proud recipient of two grant awards from the Qualifying ... Health & Human Services. QTDP tax credits are provided under ... part of the Patient Protection and Affordable Care Act of ... each grant awarded in October, 2010, and January, 2011. These ...
... April 21st issue of Science magazine, researchers at ... findings that the ozone hole, which is located over the ... Hemisphere all the way to the equator. While previous work ... flow in the high latitudes, the Columbia Engineering paper, "Impact ...
... SEGUNDO, Calif., April 21, 2011 A longtime engineer ... Space Technology Hall of Fame for his participation in ... cancer tumors and virus cultures outside the body, both ... Trinh, a senior mechanical engineer and 26-year employee at ...
Cached Biology Technology:Eigen Receives Two Grant Awards from U.S. Department of Health & Human Services 2Columbia engineering study links ozone hole to climate change all the way to the equator 2Columbia engineering study links ozone hole to climate change all the way to the equator 3Wyle Engineer Inducted Into Space Technology Hall of Fame for Work on Growing Human Cells in Space 2
(Date:11/21/2014)... According to a new market ... RFID, Access Control, Intrusion Detection, Parking Management, Under Vehicle ... Service Geography - Global Forecasts to 2020", published by ... be around $25 Billion in 2014 and is expected ... CAGR of 8.69%. Browse 116 market data ...
(Date:11/21/2014)... Nov. 19, 2014  Earlier this year Donald ... College, and one of the most prolific inventors in ... are transmitted from Smartphones to third party agencies. Spector ... has one of the earliest known patents in this ... in the military, child care, elder care and hospital ...
(Date:11/21/2014)... Calif. , Nov. 20, 2014 Strict ... driver efficiency are piloting the North American and European ... of accidents growing, gesture recognition systems that are intuitive ... a mark in the industry. New ... Automotive Gesture Recognition Market in Europe ...
Breaking Biology News(10 mins):Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 2Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 3Gesture Recognition Intensifies with the Rise of In-car Smartphone Integration, Says Frost & Sullivan 4
... observer to be busily buzzing and blooming with life,s rich ... scientists have thoroughly documentedthe distribution of species in space and ... ways. So explains Janne Soininen of the University of Helsinki ... BioScience . Soininen explores a number ...
... scientists have provided the most detailed picture yet of a ... serotonin-related antidepressants, such as Prozac. The findings, ... Proceedings of the National Academy of Sciences , open ... and anxiety with fewer side effects. Depressive disorders ...
... researchers has identified the genetic cause of an inherited ... co-led by geneticists at the University of Leeds, together ... and San Diego, should allow couples at risk of ... syndromes to be identified beforehand through genetic screening. ...
Cached Biology News:Animal study reveals new target for antidepressants 2Researchers find gene linked to birth defects 2
Mouse monoclonal [RL13] to LAP1 ( Abpromise for all tested applications). SwissProtID: Q96NW7...
Rabbit polyclonal to ECSIT ( Abpromise for all tested applications). entrezGeneID: 51295 SwissProtID: Q96HQ7...
... Antigen peptide transporter ... TAP2) (Peptide transporter PSF2) ... (PSF-2) (Peptide transporter involved ... [Source:Uniprot/SWISSPROT;Acc:Q03519] ...
Goat polyclonal to JIK ( Abpromise for all tested applications). Antigen: Synthetic peptide: HKKDHVFIRDEAGHGD, corresponding to amino acids 396-411 of Human JIK. Entrez Gene ID: 51347 Swi...
Biology Products: