Navigation Links
Fine Tuning Your Data Analysis:,,, Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms

retation of p-values can differ depending on the desired analytic stringency, one may choose to modify an alpha or a gamma cutoff. For example, if you would like to make Present calls associated with more stringent Detection p-values, you can set alpha1 to a lower level, say 0.025. Doing so will decrease the number of Present calls by converting those with p-values in the range 0.025-0.04 to Marginal calls. There will be a sensitivity vs. specificity tradeoff, since some of the calls that became Marginal may be falsely detected genes, but some may also be truly expressed genes. If you do modify the alpha or gamma cutoffs, it is advisable to maintain the p-values with the calls since they are not affected by an alpha or gamma parameter change.

References

1) Liu, W., Mei, R., Di, X., Ryder, TB., Hubbell, E., Dee, S., Webster, T., Ho, M., Baid, J., and Smeekens, SP. Analysis of high density expression microarrays with signed-rank call algorithms, manuscript, 2001.

2) Affymetrix Technical Note 1, New Statistical Algorithms for Monitoring Gene Expression on GeneChip Probe Arrays. Part Number 701097.

3) Jin, H., Yang, R., Awad, TA., Wang, F., Li, W., Williams, SP., Ogasawara, A., Shimada, B., Williams PM., Gianfranco de Feo, G., Paoni, NF. Effects of Early Angiotensin-Converting Enzyme Inhibition on Cardiac Gene Expression After Acute Myocardial Infarction. Circulation, 103:736, 2001

4) Statistical Algorithms Reference Guide. Part Number 701110.


'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
2. The New Affymetrix GeneChip Scanner 3000
3. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
4. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
5. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
6. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
7. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
8. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
9. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
10. High-Level Protein Expression, One-Column Purification, and FLAG Epitope Tagging in E. coli
11. A New Lambda Vector for Mammalian Expression
Post Your Comments:
(Date:11/21/2014)... 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") today ... September 30, 2014.  Biorem,s complete 2014 third quarter financial statements and ... Financial Summary:Three-months ended September 30, , Nine-months ended ... , 2014 , 2013 , 2014 ... 5,281 , 6,715 , 14,476 Gross profit ...
(Date:11/18/2014)... RPS Diagnostics (RPS®) – a ... announces its third annual partnership of the Centers ... About Antibiotics Week from November 17-23. , Get ... designed to highlight the coordinated efforts of CDC, ... for-profit partners to provide education about the importance ...
(Date:11/18/2014)... Urdorf, Switzerland (PRWEB) November 17, 2014 ... range of processes across the pharmaceutical, chemical, and food ... is it so important, and how do pH sensors ... measurement to assist those in process industries with these ... description of pH measurement in the process industries. The ...
(Date:11/18/2014)... (PRWEB) November 18, 2014 Alanda ... and Transparency Reporting solutions, announced today that a ... using its Consummate Providerâ„¢ solution in both the ... a Global, SaaS based, Data Quality remediation portal, ... their third party Providers improve their ability to ...
Breaking Biology Technology:Biorem Reports Third Quarter Results 2Biorem Reports Third Quarter Results 3RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 2RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 3pH Guide from METTLER TOLEDO Explains Measurement, Theory and Practice of pH Applications 2Alanda Software Completes Successful Implementation of Global Data Quality & Vendor Management Solution for Top Tier Global Biopharmaceutical Company 2
... Ontario, Nov. 6 Helix BioPharma Corp. (TSX, FSE: "HBP" ... and chief operating officer, will present at Merriman Curhan Ford,s ... PM at the Sofitel Hotel in New York City. ... product development programs L-DOS47 and Topical Interferon Alpha-2b. The ...
... - Oncolytics Biotech Inc. (TSX:ONC, NASDAQ:ONCY) ("Oncolytics" or the ... study of intravenous REOLYSIN(R) in patients with sarcomas metastatic ... 15th Annual Connective Tissue Oncology Society Meeting held in ... poster presentation, entitled "A Phase II Study of Intravenous ...
... The endangered black-footed ferrets inhabit prairie dog towns in the ... The prairie dogs are very susceptible to plague ... U.S. Fish & Wildlife Service (FWS) has implemented a ferret ... their formerly native habitat. Unfortunately, plague can sweep through ...
Cached Biology Technology:Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 2Helix BioPharma to Present at Merriman Curhan Ford's Investor Summit 2009 on November 10th 3Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 3Colorado Companies Team Up With U.S. Fish & Wildlife Service to Protect Black-Footed Ferret 2
(Date:11/7/2014)... BURBANK, Calif. , Nov. 7, 2014  In conjunction ... Burbank Police Departments, The Community Foundation of the ... BURBANK DNA JUSTICE PROJECT," a $250,000 initiative to ... successful initial phase. In its first six months of existence, ... desperately needed evidence support for local law enforcement. ...
(Date:11/6/2014)... Insilico Medicine, Inc, a Baltimore-based bioinformatics company focused ... announced a research collaboration with the international leader ... (OTC: CSBR). , "There are many companies utilizing ... Oncology,s TumorGraft technology is unique in a way ... generating vast amounts of valuable data. Our bioinformatic ...
(Date:11/4/2014)... In nature, the right amount of death at the ... density, according to new research that could help in ... wildlife stocks. , In a paper in the journal ... University researcher and European colleagues conclude that the kind ... a loss of individuals, or mortality, depends on the ...
Breaking Biology News(10 mins):Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2Insilico Medicine, Inc announces research collaboration with Champions Oncology, Inc 2When less is more: Death in moderation boosts population density in nature 2
... Stephen Moose has developed a corn plant with enormous potential ... silage, Moose said, due to a greater number of leaves ... energy crop. The gene known as Glossy 15 was ... waxy coating that acts like a sun screen for the ...
... for its health benefits. But now scientists have ... produce medicines for several autoimmune and inflammatory diseases, ... open access journal BMC Biotechnology . ... research organizations have participated in the study as ...
... knows, children love sweet-tasting foods. Now, new research ... indicates that this heightened liking for sweetness has a ... rate. "The relationship between sweet preference and growth ... demands increase. Children are programmed to like sweet ...
Cached Biology News:Doubling a gene in corn results in giant biomass 2Tobacco makes medicine 2Liking sweets makes sense for kids 2
Kit containing MACS equipment and reagents for immunopurification of proteins....
... LCMSsolution software offers an intuitive, ... instrument control, diverse data handling, ... requirements. Comprehensive functions include data ... data management in both standalone ...
... a modern flow cytometer, a scientist can ... labeled with fluorescent probes. However, the emission ... cell labeling are broader than the light ... filters. Thus, the fluorescence signal of a ...
... Sigmas FLAG Immunoprecipitation kit allows a ... of an active FLAG-tagged protein. The ... gel, which is a highly specific ... resin. The use of affinity resin ...
Biology Products: