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Fermentation Cell Culture: University Style

re pneumatic homogenization using a Microfluidics 110-EH unit, which homogenizes about one liter of resuspended cells (about 300 grams) in under three minutes. We are also installing a bead mill (Glen Mills) for continuous cell disruption under chilled, low-pressure conditions. The facility also houses a whole-room chilled water system which allows us to tap into an in-room heat exchanger (System III, Thermo Electron) at any point along the walls to cool any of the devices we have installed to ~5C.

BioPharm: If a colleague is considering using your strategy (a fermentor), what are your recommendations?

Stevens: I would strongly recommend that investigators interested in this type of facility installation meet with representatives from the manufacturers and consult architects to determine their needs and the best way to address them. For example, an important consideration for NIH protocol compliance was the containment of a bioproduct breach, either from a fermentor or centrifuge spill, or from a processing catastrophe. This necessitated the design of a containment system incorporated into the room, essentially making the whole room a waterproof containment pool. Containment breaches and routine facility cleaning and maintenance are performed by chemically sterilizing the entire facility before passing inactivated material down an otherwise sealed floor drain.

BioPharm: Does your laboratory have any business partnerships within the fields of biopharmaceuticals, traditional pharmaceuticals, or government? '"/>

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