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Fermentation Cell Culture: University Style

400 liters, I am confident that further scalability to larger volumes could occur should our experiments call for it.

BioPharm: How have your processes and results changed since using a fermentor?

Stevens: Using the fermentor has allowed my research to advance in directions previously thought unimaginable prior to obtaining the fermentor. Although it is possible to grow hundreds of liters of cells without a fermentor (albeit an extremely tedious and time consuming proposition), we find that the reproducibility and dynamic monitoring of variables such as dissolved oxygen, pH, temperature, and agitation speed allow us to predictably and reproducibly generate useful biomass for our experiments.

BioPharm: Is the fermentor integrated with any other technology in the laboratory/ facility?

Stevens: Our facility has been designed for maximum flexibility and ease of use. In addition to the fermentor, there is a process steam generator (Electro Steam Generator). We use a CEPA Z-60 continuous-flow centrifuge for biomass harvesting. This centrifuge allows us to harvest 400 liters of culture volume in under an hour. We also have installed a 12-liter, smallscale fermentor (NBS) that can be connected through an in situ sterilizable connector (custom part, NBS) to feed the large fermentor.

To process the material obtained from the fermentor, we have the option of high-pressu
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