Navigation Links
Fermentation Cell Culture: University Style

although using the fermentor typically allows for faster and more dense culture growth, the procedures scale directly and the resulting product is identical to what is produced from smaller-scale cultures. We are currently attempting to optimize culture conditions with oxygen supplementation to increase the biomass yield at the end of the culture growth. With the equipment we use, there have been no challenges to overcome in scaling to this level, other than learning how to operate the equipment.

BioPharm: How customary is it for a college or university laboratory to use a fermentor to grow culture?

Stevens: In researching the purchase of the equipment for our facility, we found that many academic laboratories, departments, and colleges that used large-scale fermentation at one time have discontinued their use in the last several years. In the past decade or more, laboratories interested in milligram quantities of protein have dispensed with large-scale growth of microorganisms as bacterial over-expression systems have progressed to the point where hundreds of milligrams of product can often be purified from one to five liters of culture. In the current age of structural genomics where the low-hanging fruit of easily expressed single polypeptides will have their structures determined rapidly, these smallscale cultures are ideal.

For investigators like myself who study polypeptides of up to 280 kD contained in macromolecular complexes composed of dozens of polypeptides and several RNAs, those structures will only be determined through the brute-force techniques we use to purify them from their natural hosts. Several factors preven
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Fermentation Cell Culture University Style

(Date:8/27/2014)... BOSTON , Aug. 27, 2014 Rhythm, ... diseases and genetic deficiencies that result in metabolic disorders, ... on Form S-1 with the U.S. Securities and Exchange ... of shares of its common stock. The number of ... the offering have not yet been determined. ...
(Date:8/27/2014)... 2014 Reportlinker.com announces that a ... its catalogue: Global Biodegradable Packaging ... http://www.reportlinker.com/p02316661/Global-Biodegradable-Packaging-Materials-Market-2014-2018.html About Biodegradable Packaging ... readily decomposed by the action of microorganisms. ... in terms of raw materials, production technology, ...
(Date:8/27/2014)... of nature,s mysteries is how plants survive impact ... the sun,s rays, while using this energy for ... in the plant,s blades quickly dissipate the energy ... quakes. Researchers at DTU Physics have now managed ... algae and bacteria contain light-absorbing proteins which play ...
(Date:8/27/2014)... , Aug. 27, 2014 Reportlinker.com ... available in its catalogue: Global Chelating ... About Chelating Agent A chelating ... covalent bonds with metal ions, thereby forming a ... some metal-ions have on chemical processes, formulations, and ...
Breaking Biology Technology:Rhythm Files Registration Statement for Proposed Initial Public Offering 2Global Biodegradable Packaging Materials Market 2014-2018 2Global Biodegradable Packaging Materials Market 2014-2018 3Global Biodegradable Packaging Materials Market 2014-2018 4Global Biodegradable Packaging Materials Market 2014-2018 5Global Biodegradable Packaging Materials Market 2014-2018 6Global Biodegradable Packaging Materials Market 2014-2018 7DTU researchers film protein quake for the first time 2Global Chelating Agents Market 2014-2018 2Global Chelating Agents Market 2014-2018 3Global Chelating Agents Market 2014-2018 4
... Alfalight , a firm that makes high-power ... has established ties with Chinese distributor Sanetronic Company ... Hong Kong. The company was founded in 1987 as ... has eight offices in China, including Beijing, Shanghai, Shenzhen, ...
... research, nanotechnology, farm waste management and produce storage have emerged ... Transfer Award. , ,Judges for the contest have winnowed the ... The $5,000 grand prize will be presented to the winner ... in Milwaukee. , ,The finalists are: , , GHD ...
... The Joint Finance Committee rejected Governor Jim Doyle's proposal for ... The proposal, a plan to charge consumers for their music, ... several proposals turned down by the budget review panel. , ... on electronic entertainment called by some detractors the "iPod ...
Cached Biology Technology:Five remaining firms vie for Tech Transfer award 2Five remaining firms vie for Tech Transfer award 3Finance Committee rejects extra tax on Internet buys 2Finance Committee rejects extra tax on Internet buys 3
(Date:8/27/2014)... effective doses of the HIV drug maraviroc, a new ... completed before the drug was licensed in 2007, included ... key to removing maraviroc from the body. The current ... protein including nearly half of African-Americans end ... those who lack the protein even when given the ...
(Date:8/27/2014)... neural circuits in the brain of mice, scientists ... The research, led by Howard Hughes Medical Institute ... Technology (MIT), reveals that the connections between the ... about an experience and the part of the ... experience are malleable. , Altering those connections ...
(Date:8/27/2014)... to listen carefully to someone, the first thing we ... is stop moving altogether. This strategy helps us hear ... movements. , This interplay between movement and hearing also ... evidence has long suggested that the brain,s motor cortex, ... gives rise to our conscious perception of sound. , ...
Breaking Biology News(10 mins):Dosage of HIV drug may be ineffective for half of African-Americans 2Researchers change the emotional association of memories 2Researchers change the emotional association of memories 3Researchers change the emotional association of memories 4Stop and listen: Study shows how movement affects hearing 2Stop and listen: Study shows how movement affects hearing 3
... ORLANDO, Fla. Soy food consumption did not ... survivors of breast cancer, according to the results of ... 2011, held April 2-6. Researchers investigated the association ... survivors, using data from a multi-institution collaborative study, the ...
... America (ESA) has selected 37 insect-science symposia for its ... http://entsoc.org/entomology2011 ). The symposia will address important ... Disorder, insect control, invasive species, medical and veterinary entomology, ... BEES AND COLONY COLLAPSE DISORDER Bee ...
... premature aging of immune cells, which may make people more ... new UCSF-led study. The findings indicate that accelerated ... is dependent upon how long someone is depressed, particularly if ... March 2011 by the journal PLoS One and ...
Cached Biology News:Soy isoflavones not a risk for breast cancer survivors 2Entomology 2011 symposia selected 2Entomology 2011 symposia selected 3Study finds link between chronic depression and accelerated immune cell aging 2Study finds link between chronic depression and accelerated immune cell aging 3
... Rotofor purification system with PowerPac 3000 power ... separation and purification by liquid-phase isoelectric focusing ... (18 ml) and standard (60 ml) focusing ... chambers, PowerPac 3000 power supply, 10 ml ...
... Imager VersaDoc MP 4000 System is ... imaging system capable of supporting a ... fluorescence, chemiluminescence, densitometry, and chemiflourescence. ... allow imaging of multiplexed fluorescent samples, ...
...
...
Biology Products: