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Fermentation Cell Culture: University Style

although using the fermentor typically allows for faster and more dense culture growth, the procedures scale directly and the resulting product is identical to what is produced from smaller-scale cultures. We are currently attempting to optimize culture conditions with oxygen supplementation to increase the biomass yield at the end of the culture growth. With the equipment we use, there have been no challenges to overcome in scaling to this level, other than learning how to operate the equipment.

BioPharm: How customary is it for a college or university laboratory to use a fermentor to grow culture?

Stevens: In researching the purchase of the equipment for our facility, we found that many academic laboratories, departments, and colleges that used large-scale fermentation at one time have discontinued their use in the last several years. In the past decade or more, laboratories interested in milligram quantities of protein have dispensed with large-scale growth of microorganisms as bacterial over-expression systems have progressed to the point where hundreds of milligrams of product can often be purified from one to five liters of culture. In the current age of structural genomics where the low-hanging fruit of easily expressed single polypeptides will have their structures determined rapidly, these smallscale cultures are ideal.

For investigators like myself who study polypeptides of up to 280 kD contained in macromolecular complexes composed of dozens of polypeptides and several RNAs, those structures will only be determined through the brute-force techniques we use to purify them from their natural hosts. Several factors preven
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