Navigation Links
Femtogram Sensitivity of the Antipsychotic Drugs Raclopride and Ritanserin Using a Varian 1200L LC/MS/MS

J. Beck and C. Schmidt
Varian, Inc.

Introduction

Ritanserin (Tisterton) is a serotonin-2 antagonist drug shown to be effective in the treatment of anxiety and depression1 while Raclopride acts as a dopamine D2 receptor antagonist.2 These two compounds were used to demonstrate low-level sensitivity of the Varian 1200L LC/MS/MS in selected reaction monitoring mode (SRM). Studies have shown LC/MS to be a useful tool in the quantitation of low levels of Raclopride.3

Instrumentation

Varian 1200L LC/MS/MS equipped with ESI source

Materials and Reagents

Ritanserin ordered from Sigma-Aldrich Corp.,Catalog Number R-103

S-(-)-Raclopride ordered from Sigma-Aldrich Corp.,Catalog Number R-121

All other chemicals are reagent grade or HPLC grade

Sample Preparation

Milligram quantities were prepared and dissolved in a 50:50 mixture of acetonitrile/water with 0.1% formic acid. Serial dilutions were made from each stock solution prior to flow injection analysis (FIA). Samples were injected into a mobile phase of 50:50 acetonitrile/0.1% formic acid which was pumped directly into the mass spectrometer at 0.2 mL/min. A 5 μL loop was used for the injections and the injections were repeated five times for each concentration level.

Results and Discussion

Raclopride Five replicates at five different concentration levels were run. FIA injections of Raclopride at its lowest detectable limit, 100 pg/mL (500 fg on-column), is shown in Figure 1. At the lowest level, the S/N ratio is approximately 10:1 for the raw data. Also, a plot at a higher concentration of 10 ng/mL is included for comparison (Figure 2). The calibration cur
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Improved Sensitivity for Staining RNA with SYBR Gold Stain
2. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
3. Increase the Power and Sensitivity for Your cDNA Synthesis with the New Transcriptor Reverse Transcriptase
4. Increased Sensitivity in Microarray Analysis
5. Maintaining High Sensitivity when Working with Small-Volume Samples
6. High Sensitivity, Wide Dynamic Range, Horseradish Peroxidase (HRP) ELISA Using the LMax Microplate Luminometer (MaxLine Application Note #41)
7. Sensitivity and Quantitation using a Finnigan LCQ Deca XP Plus Ion Trap Mass Spectrometer
8. Increased Analyte Sensitivity through the Utility of Enhanced Mass-Resolution on the FinniganTSQ Quantum Discovery
9. Trace Analysis Optimizing Sensitivity Finnigan Surveyor PDA Detector
10. A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity
11. Simultaneous Screening of 23 Drugs of Abuse in Oral Fluid Using an LC/MS/MS Method
Post Your Comments:
(Date:10/22/2014)... Massachusetts (PRWEB) October 22, 2014 ... announced the release of a new infographic, Understanding ... to create a beautifully designed, easy to follow informative ... transmission, areas of outbreak, symptoms and prevention. , "As ... discovered that the 2014 Ebola outbreak represents not only ...
(Date:10/22/2014)... 22, 2014 According to new ... Service Type (Consulting, Integration, Deployment & Support), by ... Model (Public, Private, Others (Hybrid & Community)) - ... and segments the Cloud Professional Services Market into ... of revenues. It also identifies the drivers and ...
(Date:10/22/2014)... HONG KONG , Oct. 22, 2014  aTyr ... today that rare disease expert John C. McKew ... Dr. McKew brings more than two decades of expertise ... National Institutes of Health, Wyeth Research and Genetics Institute, ... lead aTyr,s efforts to expand and translate its novel ...
(Date:10/20/2014)... Oct. 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or ... brings Q3 orders to $5.8 million and provides a good start ... North America and one in the ... new projects at record levels," said Peter Bruijns , President ... the end of Q3 than they have been for any complete ...
Breaking Biology Technology:Involution Studios' Ebola Infographic Provides Key Disease Information and Statistics at a Glance 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... 24, 2011 Accera, Inc., a biotech ... has donated $50,000 to the Alzheimer,s Foundation of America ... (NMSD) during National Alzheimer,s Disease Awareness Month.  These funds ... educate and support individuals with Alzheimer,s disease (AD) and ...
... Protein Corporation (NYSE symbol: OME), a nutritional ingredient company ... and specialty fish meal products, today announced that it ... in connection with its omega-3 fish oil and omega-3 ... is an analytical services and biotechnology consulting company that ...
... Silence Therapeutics plc (AIM: SLN) ("Silence"), a ... that it has entered into an agreement with ... microRNA-based therapeutics for cancer, to assess the delivery ... systems for Mirna,s novel microRNAs. Under ...
Cached Biology Technology:Accera, Inc. Donates $50,000 to the Alzheimer's Foundation of America for National Memory Screening Day 2Accera, Inc. Donates $50,000 to the Alzheimer's Foundation of America for National Memory Screening Day 3Omega Protein Announces Retention of Lipid Technologies, LLC 2Silence Therapeutics Signs Collaboration With Mirna Therapeutics to Evaluate Delivery of Novel microRNA Therapeutics 2Silence Therapeutics Signs Collaboration With Mirna Therapeutics to Evaluate Delivery of Novel microRNA Therapeutics 3Silence Therapeutics Signs Collaboration With Mirna Therapeutics to Evaluate Delivery of Novel microRNA Therapeutics 4Silence Therapeutics Signs Collaboration With Mirna Therapeutics to Evaluate Delivery of Novel microRNA Therapeutics 5Silence Therapeutics Signs Collaboration With Mirna Therapeutics to Evaluate Delivery of Novel microRNA Therapeutics 6
(Date:10/18/2014)... and stress-related psychiatric disorders are associated with ... molecular mechanisms underlying this relation are unknown. ... development of targeted preventive strategies and new ... This work is presented at the European ... an international group of researchers from Germany ...
(Date:10/17/2014)... for the first time how bacteria can grow directly ... the opportunity to get tremendous insights into bacteria behavior ... discovered the bacterial growth in chronic lung infections among ... by the immune cells. The researchers discovered the immune ... bacteria, forcing the bacteria to switch to a much ...
(Date:10/16/2014)... the bowel lining develops and, in the process, suggested ... The researchers produced evidence that stem cells are responsible ... feature of the bowel lining, and believe these stem ... finding as scientists are still divided on the stem ... Wee Tan and Professor Tony Burgess from the Walter ...
Breaking Biology News(10 mins):Researchers find why depression and aging linked to increased disease risk 2Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2Cryptic clues drive new theory of bowel cancer development 2
... American ecosystems over the past 150 years have caused coyotes ... southwestern deserts of North America to habitats throughout the United ... in the Journal of Mammalogy , researchers from the ... used DNA from coyote scat (feces) to trace the route ...
... The fictitious storybook character Dr. Doolittle was ... an assistant professor of psychology at Rochester Institute ... focuses on object discrimination in goldfish and echolocation ... the mysteries of animal perception and cognition. ...
... to trick ourselves into believing we are full even as our ... the November issue of the Cell Press journal Cell Metabolism ... to go with our guts just two gut hormones, that ... could be the long-sought answer to healthy eating, according to the ...
Cached Biology News:New genetic evidence confirms coyote migration route to Virginia and hybridization with wolves 2Go fish! RIT psychology professor trains goldfish for object perception research 2Simple gut hormone combo makes our brains think we're full 2
... the premium quality siRNAs you need for ... be readily ordered online by providing either ... providing the sense and antisense siRNA sequences. ... Silencer Pre-designed siRNAs, which are already designed ...
Request Info...
Bovine Serum Albumin Heat Shock Diagnostic Grade Powder...
, Premium quality synthesis and purification , You provide the sequence, we'll do the rest , Rigorous quality control , Your choice of purification method, amount and format , Available annealed...
Biology Products: