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Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays

good dose-response over the range from 12 to 0.6 g/ml. The same was true for the phosphoproteins in each of the treated samples. These results emphasize the importance of establishing the dose-response relationship between the concentration of each protein target and the corresponding signal when a specific cell lysate sample is tested. These results demonstrate that multiplexing a total assay with five phosphoprotein assays can detect differences among samples with different protein concentrations.


Conclusions
The results of this study successfully answered the questions posed, demonstrating that:
A total target assay can be multiplexed with phosphoprotein assays, provided the target proteins are different
A total target assay can detect differences in protein concentrations among samples

xMAP is a trademark of Luminex Corporation. The Bio-Plex suspension array system includes fluorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation.

Information in this tech note was current as of the date of writing (2005) and not necessarily the date this version (Rev A, 2005) was published.


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