( NA Polymerase with anti-Taq antibody; ...)FastStart Taq DNA Polymerase

NA Polymerase with anti-Taq antibody; F: Supplier B, antibody-modified hot start polymerase.
Figure 2: Sensitivity comparison when amplifying a single-copy gene. Varying amounts of human genomic DNA were used for amplification of a single 375 bp fragment from the tissue plasminogen activator (tPA) gene. Manufacturers' recommended initial product-activation times were used when applicable. Cycling conditions: 40 cycles (95C / 30 seconds - 55C / 30 seconds - 72C / 60 seconds) final extension at 72C for 7 minutes. A: FastStart Polymerase; B: Taq DNA Polymerase; C: Supplier A, modified hot start polymerase, buffer I; D: Supplier A, modified hot start polymerase buffer II; E: Taq DNA Polymerase with anti-Taq antibody; F: Supplier B, antibody-modified hot start polymerase. Key advantages

• Minimize optimization difficulties in all types of PCR with FastStart Polymerases specially designed and optimized buffers.
• Achieve results with difficult templates using the optimized GC-RICH Resolution Solution
• Amplify templates up to 3 kb and even difficult templates (e.g., high GC content, secondary structure) better than other hot start systems
• Produce results across a wide range of magnesium concentrations
• Eliminate wax barriers, beads, hot start antibodies, manual hot start, and the need to set up PCR reactions on ic

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