( Acrobat PDF format and must...)FastStart Taq DNA Polymerase

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Application FastStart Taq DNA Polymerase is an ideal tool for "Hot Start" PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step. Since it is inactive at low temperatures, FastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. FastStart Taq DNA Polymerase delivers superior results if used for:

• Amplification of genomic DNA and cDNA targets up to 3 kb long with high specificity, sensitivity, and yield
• Multiplex PCR
• Difficult templates e.g. secondary structures or GC-rich sequences
• Carry-over prevention
• Automated PCR e.g. handling at room temperatures

Experimental results
Figure 1: Specificity and sensitivity comparison in PCR using commercially available hot start systems. Varying amounts of human genomic DNA were used for the amplification of a single 130 bp fragment from the tissue plasminogen activator (tPA) gene. Manufacturers' recommended initial product activation times were used when applicable. Cycling conditions: 35 cycles (95C / 30 seconds - 60C / 30 seconds -72C / 60 seconds) final extension at 72C for 7 minutes. A: FastStart Polymerase; B: Taq DNA Polymerase; C: Supplier A, modified hot start polymerase, buffer I; D: Supplier A, modified hot start polymerase buffer II; E: Taq D
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