FastStart Taq DNA Polymerase has been developed by Roche to make Polymerase Chain Reaction (PCR) more specific and sensitive in a convenient and rapid way. With FastStart Taq DNA Polymerase, "Hot Start" PCR can be applied for genomic DNA and cDNA templates, eliminating the extra handling steps or additional time required associated with up to now known "Hot Start" methods.
FastStart Taq DNA Polymerase is a thermostable, modified form of recombinant Taq DNA Polymerase. It is inactive at temperatures below 75C, but can be activated by a 4 min 95C incubation step. The combination of FastStart Taq DNA Polymerase and the optimized PCR buffer minimizes non-specific amplification products and primer dimers allowing highest sensitivity.
The provided GC-RICH solution, a PCR additive that facilitates amplification of difficult templates by modifying the melting behavior, will improve PCR performance on templates rich in secondary structures or GC content.