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FastStart Taq DNA Polymerase

for "Hot Start" PCR amplification of up to 3 kb RNA targets Cat. No. 2 158 264 50 units for 25 PCR reactions
Cat. No. 2 032 902 100 units for 50 PCR reactions
Cat. No. 2 032 929 2 250 units for 250 PCR reactions
Cat. No. 2 032 937 4 250 units for 500 PCR reactions
Cat. No. 2 032 945 10 250 units for 1250 PCR reactions
Cat. No. 2 032 953 20 250 units for 2500 PCR reactions Description

FastStart Taq DNA Polymerase has been developed by Roche to make Polymerase Chain Reaction (PCR) more specific and sensitive in a convenient and rapid way. With FastStart Taq DNA Polymerase, "Hot Start" PCR can be applied for genomic DNA and cDNA templates, eliminating the extra handling steps or additional time required associated with up to now known "Hot Start" methods.

FastStart Taq DNA Polymerase is a thermostable, modified form of recombinant Taq DNA Polymerase. It is inactive at temperatures below 75C, but can be activated by a 4 min 95C incubation step. The combination of FastStart Taq DNA Polymerase and the optimized PCR buffer minimizes non-specific amplification products and primer dimers allowing highest sensitivity.

The provided GC-RICH solution, a PCR additive that facilitates amplification of difficult templates by modifying the melting behavior, will improve PCR performance on templates rich in secondary structures or GC content.

For further information please download the FastStart product flyer (950 Kbytes PDF file). Please note that this file is in Acrobat PDF format and must be viewed, searched, and printed with version 3.0 or higher of the free Adobe Acrobat Reader.

Application FastStart Taq DNA Polymerase is an ideal tool for "Hot Start" PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step. Since it is inactive at low temperatures, FastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. FastStart Taq DNA Polymerase delivers superior results if used for:
  • Amplification of genomic DNA and cDNA targets up to 3 kb long with high specificity, sensitivity, and yield
  • Multiplex PCR
  • Difficult templates e.g. secondary structures or GC-rich sequences
  • Carry-over prevention
  • Automated PCR e.g. handling at room temperatures
Experimental results
Figure 1: Specificity and sensitivity comparison in PCR using commercially available hot start systems.
Varying amounts of human genomic DNA were used for the amplification of a single 130 bp fragment from the tissue plasminogen activator (tPA) gene. Manufacturers' recommended initial product activation times were used when applicable. Cycling conditions: 35 cycles (95C / 30 seconds - 60C / 30 seconds -72C / 60 seconds) final extension at 72C for 7 minutes. A: FastStart Polymerase; B: Taq DNA Polymerase; C: Supplier A, modified hot start polymerase, buffer I; D: Supplier A, modified hot start polymerase buffer II; E: Taq D NA Polymerase with anti-Taq antibody; F: Supplier B, antibody-modified hot start polymerase.
Figure 2: Sensitivity comparison when amplifying a single-copy gene.
Varying amounts of human genomic DNA were used for amplification of a single 375 bp fragment from the tissue plasminogen activator (tPA) gene. Manufacturers' recommended initial product-activation times were used when applicable. Cycling conditions: 40 cycles (95C / 30 seconds - 55C / 30 seconds - 72C / 60 seconds) final extension at 72C for 7 minutes. A: FastStart Polymerase; B: Taq DNA Polymerase; C: Supplier A, modified hot start polymerase, buffer I; D: Supplier A, modified hot start polymerase buffer II; E: Taq DNA Polymerase with anti-Taq antibody; F: Supplier B, antibody-modified hot start polymerase. Key advantages
  • Minimize optimization difficulties in all types of PCR with FastStart Polymerases specially designed and optimized buffers.
  • Achieve results with difficult templates using the optimized GC-RICH Resolution Solution
  • Amplify templates up to 3 kb and even difficult templates (e.g., high GC content, secondary structure) better than other hot start systems
  • Produce results across a wide range of magnesium concentrations
  • Eliminate wax barriers, beads, hot start antibodies, manual hot start, and the need to set up PCR reactions on ic


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