Navigation Links
Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood

isolations were performed under identical conditions using the genomic DNA isolation procedure described in the kits manual (Figure 2). PCR analysis was performed using 1 ml of the isolated genomic DNA as template and primers specific for 1-antitrypsin (a1-AT). The results demonstrate that 1 ml of genomic DNA template isolated from a range of blood volumes is sufficient for amplification of the expected 980-bp PCR product.

Amplification of Various PCR Target Sizes

Fig.3

Genomic DNA isolated from blood using the DNA Stat kit is high molecular weight (approximately 40 kb in size) (Figure 3). To further test the integrity of the isolated genomic DNA, 1-ml aliquots of a 50-ml prep were used as templates for amplifying a range of PCR target sizes. We used primers specific to aldolase B (Aldo: 250 bp), T-cell receptor b-chain gene (TCRb: 500 bp), and a1-antitrypsin (a1-AT: two primer sets amplifying 980-bp and 5.2K-bp PCR targets) in PCR reactions with 1 ml al of genomic DNA. Genomic DNA isolated using the DNA Stat kit is suitable for amplifying PCR targets ranging in size from 250 bp to 5.2 kb (Figure 4).

Fig.4

We also assessed genomic DNA quality by amplifying PCR targets on each of the human chromosomes. Primers designed to amplify targets containing simple sequence repeats on chromosomes 1 through 22 plus X and Y were used for this analysis (Human MapPairs primers, Research Genetics: TCRb primers were used for chromosome 7). The resulting PCR products ranged in size from 202 bp to 500 bp. From 100 ml of whole blood, 1 ml of DNA was used as a template for PCR
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. High-Throughput Isolation of Total RNA
2. Simple Isolation of RNA from Tissue and Cultured Cells
3. Fast Isolation of Total RNA from Small Numbers of Cells
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. Isolation of Microorganisms
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Basic Plasmid DNA Isolation Protocol
10. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
11. RNA-free Plasmid DNA Isolation Protocol
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Fast and Easy Isolation PCR Ready Genomic DNA from Whole Blood

(Date:10/27/2014)... BOSTON, MA (PRWEB) October 27, 2014 ... and profit. SoundConnect , an industry leading ... for its partners and agents to deliver cloud-based ... and accelerate growth opportunities. , With SoundConnect’s ... for every twenty-five video and/or web conferencing licenses ...
(Date:10/27/2014)... 27, 2014 The report “Smart ... Traffic Management, Communication, Monitoring), and by Display (Variable ... 2019” segments the global market into various sub-segments ... also identifies the drivers and restraints for this ... , Browse 75 market tables and 37 figures ...
(Date:10/27/2014)... Pa. , Oct. 27, 2014 ... Foundation of America (CCFA) announced today the launch ... , an educational campaign designed to ... diseases (IBD) on the paired role of nutrition ... To view the multimedia assets associated with this ...
(Date:10/26/2014)... 26, 2014 The report "Data ... Network Security Equipment, WAN Optimization Appliances]: Global Advancements, ... the data center networking market into various segments ... The report also identifies the factors driving this ... it along with the future roadmaps. , Browse ...
Breaking Biology Technology:SoundConnect Unveils Q4 Partner Incentives 2Smart Highway Market by Technology, and Display Expected to Reach $27.992.0 Million by 2019- New Report by MarketsandMarkets 2Smart Highway Market by Technology, and Display Expected to Reach $27.992.0 Million by 2019- New Report by MarketsandMarkets 3Smart Highway Market by Technology, and Display Expected to Reach $27.992.0 Million by 2019- New Report by MarketsandMarkets 4Celebrity Cook Sunny Anderson Teams Up With Janssen And Crohn's & Colitis Foundation Of America To Launch Educational Campaign 2Celebrity Cook Sunny Anderson Teams Up With Janssen And Crohn's & Colitis Foundation Of America To Launch Educational Campaign 3Celebrity Cook Sunny Anderson Teams Up With Janssen And Crohn's & Colitis Foundation Of America To Launch Educational Campaign 4Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 2Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 3Data Center Networking Market by Ethernet Switches, SAN & Routers Worth $21.85 Billion by 2018 - New Report by MarketsandMarkets 4
... news item is out of step with public opinion in ... state of Wisconsin grants $1 million and loans another $1 ... UW-Madison researcher so renowned that the words "Nobel Prize" practically ... drug discovery. , ,• The National Institutes of Health, the ...
... records have led to some adoption of the technology but ... ,Then Hurricane Katrina slammed into the Gulf Coast, destroying countless ... ,Katrina, and her followup Rita, may not only have changed ... focus of debate on electronic healthcare records. It is becoming ...
... -- Research scientist Seth Dobrin, Ph.D., has been named ... Genotyping Service of the Marshfield Clinic Research Foundation. , ,He ... to problems of mental health and development of new genetic ... discoveries he made earlier in his career as well as ...
Cached Biology Technology:Stem cell bank announcement validates quality of Wisconsin research 2Stem cell bank announcement validates quality of Wisconsin research 3Katrina's destruction likely to speed up electronic healthcare records adoption 2Katrina's destruction likely to speed up electronic healthcare records adoption 3Katrina's destruction likely to speed up electronic healthcare records adoption 4
(Date:10/28/2014)... differences in moving activity in a novel environment ... angling, according to an experimental study completed at ... Game and Fisheries Research Institute. The study used ... and authentic angling trials to analyse if behaviours ... reared in traditional and enriched hatchery rearing environments. ...
(Date:10/28/2014)... and citrus fruits and juices are associated with a ... research from the University of East Anglia (UEA). ... foods containing flavonols and flavanones (both subclasses of dietary ... cancer, the fifth-leading cause of cancer death among women. ... of 171,940 women aged between 25 and 55 for ...
(Date:10/27/2014)... are an indigenous species of the West Antarctic Peninsula ... Earth. Since 1950, the average annual temperature in the ... and 6 degrees Celsius during winter. , As ... dry, polar system to a warmer, sub-polar system with ... a connection between local weather conditions and the weight ...
Breaking Biology News(10 mins):Fish 'personality' linked to vulnerability to angling 2Tea and citrus products could lower ovarian cancer risk, new UEA research finds 2University of Delaware study connects penguin chick weights to local weather conditions 2University of Delaware study connects penguin chick weights to local weather conditions 3
... they used fluorescent molecules to "tag" DNA and monitor a ... rearranging genetic material in some types of cells. ... light on how DNA loops form, but also might be ... shuffle genetic material, such as HIV. Until now, scientists ...
... more DNA regions linked to type 2 diabetes have been ... to over 60. The study provides a fuller picture ... with some clear patterns emerging. The international team, ... Institute of Harvard and MIT, and the University of Michigan, ...
... affordable and accessible, we need to pay more attention to ... and the fact that we don,t yet understand what ... Hastings Center Report . The authors are current or former ... Most analyses of the ethical issues raised by whole ...
Cached Biology News:Scientists use light to 'tag and track' genetic processes 210 new diabetes gene links offer picture of biology underlying disease 210 new diabetes gene links offer picture of biology underlying disease 3Prenatal whole genome sequencing: Just because we can, should we? 2Prenatal whole genome sequencing: Just because we can, should we? 3
Request Info...
... Biosearch Technologies has been at the ... accelerate the discovery and application of ... FRET probes and primers or 100 ... applications, Biosearch can meet your most ...
... Protein Concentration Basement Membrane Extract Cultrex ... in vivo applications where higher protein ... gel strength, and elevated levels of ... advantage of lot-to-lot consistency and controlled ...
Cultrex 3D Culture Matrix Laminin I....
Biology Products: