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Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood

the DNA Stat blood kit is the unique design of the spin cups, which preferentially bind white blood cells (WBC) from a whole blood sampleol (Figure 1), a hypotonic solution is added to a microspin cup seated in a 2-ml collection tube. A whole blood sample (25 l to 200 l) is then added to this solution, and the red blood cells (RBC) are lysed. Centrifugation removes RBC and serum protein contaminants while the WBC are retained. The WBC are lysed by adding a chaotropic salt solution, and the released genomic DNA is bound to the DNA Stat spin cup. Residual contaminants are removed with two wash steps, and the genomic DNA is then eluted in a small volume of buffer. The resulting DNA is high molecular weight and ready for use as a template in PCR reactions. Each prep yields enough DNA for up to 50 PCR reactions with 1 ml to 5 ml of DNA, providing sufficient template for most reactions.

When comparing contemporary protocols with Stratagenes kit (Table 1), the DNA Stat method demonstrates several advantages: no pelleting of WBC before lysis, no protease digestion, and room temperature incubations. A single microspin cup is all that is necessary to carry out the isolation, one simple protocol is used for a range of blood volumes, and the entire isolation takes only 40 minutes.

Convenient DNA Isolation from Whole Blood

Fig.2

The DNA Stat kit can be used to isolate DNA from a range of whole blood volumes without making any changes to the protocol. Other commercially available blood DNA isolation kits are optimized for a specific blood volume, and any change in starting volume requires protocol modification. All
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