the DNA Stat blood kit is the unique design of the spin cups,
which preferentially bind white blood cells (WBC) from a whole blood sampleol
), a hypotonic solution is added to a microspin cup seated in a 2-ml
collection tube. A whole blood sample (25 l to 200 l)
is then added to this solution, and the red blood cells (RBC) are lysed.
Centrifugation removes RBC and serum protein contaminants while
the WBC are retained. The WBC are lysed by adding a chaotropic salt
solution, and the released genomic DNA is bound to the DNA Stat spin cup.
Residual contaminants are removed with two wash steps, and the genomic
DNA is then eluted in a small volume of buffer. The resulting DNA
is high molecular weight and ready for use as a template in PCR reactions.
Each prep yields enough DNA for up to 50 PCR reactions with 1 ml to 5
ml of DNA, providing sufficient template for most reactions.
When comparing contemporary protocols with Stratagenes kit (Table
1), the DNA Stat method demonstrates several advantages: no pelleting
of WBC before lysis, no protease digestion, and room temperature incubations.
A single microspin cup is all that is necessary to carry out the isolation,
one simple protocol is used for a range of blood volumes, and the entire
isolation takes only 40 minutes.
Convenient DNA Isolation from Whole Blood
The DNA Stat kit can be used to isolate DNA from a range of whole blood
volumes without making any changes to the protocol. Other commercially
available blood DNA isolation kits are optimized for a specific blood
volume, and any change in starting volume requires protocol modification.
All Page: All 1 2 3 4 5 Related biology technology :1
. High-Throughput Isolation of Total RNA2
. Simple Isolation of RNA from Tissue and Cultured Cells3
. Fast Isolation of Total RNA from Small Numbers of Cells4
. Comparison of Growth Techniques and Media for the Purpose of Plasmid
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Perfectprep Plasmid Mini
. Isolation of Microorganisms6
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. Mouse Tail Genomic DNA Isolation Protocol(1)8
. Genomic DNA Isolation Protocol(1,2,3)9
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. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock
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