( ...)Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood

Novel method streamlines blood DNA purification for PCR applications

Sylvia Norman Jeff Braman

Stratagene

Using the new DNA Stat blood kit, it is easy to isolate PCR-quality human genomic DNA from whole blood with a uniquely designed spin-cup. Genomic DNA is quickly purified from 25 l to 200 l of whole blood with fewer steps and less handling compared to other methods; the resulting DNA is approximately 40 kb. The PCR targets tested and amplified ranged from 250 bp to 5.2 kb. To demonstrate that all human chromosomes were present in DNA isolated from human blood, we amplified simple sequence repeat markers. This novel isolation procedure was also shown to be successful in a clinical reference laboratory setting.

Genomic DNA isolated from whole blood has a broad range of PCR analysis applications that include typing HLA class I and II alleles,^1,2,3 screening neonates,⁴ detecting tumor cells,⁵ and studying allelic discrimination.^6,7,8 Commercially available genomic DNA isolation kitswe designed a simple method that does not require multiple transfer steps, the use of proteases with their attendant incubations, phenol-chloroform extractions, alcohol precipitation, or additional incubations above room temperature.

DNA Stat Blood Kit Protocol

Fig.1

The key to
'"/>

Source: