Navigation Links
Fast Multi-Residue Pesticide Analysis in Soil and Vegetable Samples

The strict detection limits that are required for the quantitation of pesticide residues in food and environmental samples requires the use of very sensitive, selective, and rugged LC/MS/MS technologies. In this application note the benefits of using LC/MS/MS for this type of analysis is described. In particular the role of the collision cell in the MS/MS experiment is addressed. A method for the simultaneous multiresidue screening of 55 pesticides in both soil and leek extracts in less than 15 minutes is described.

Materials and methods

The 55 pesticides are listed in Table 1. Standard solutions (0.1 250 g.L-1 ) are prepared in a mixture of 50/50 (v/v) Water/Acetonitrile. Leek samples preparation consists of adding 250 ml acetone to 25 gram of leek. 20 ml of this solution is cleaned up by using liquid/liquid extraction (2 x 50 ml dichloromethane). After evaporation to dryness the extract is reconstituted in 2 ml 50/50 (v/v) water/ACN and spiked with a solution containing 10 g.L-1 of each of the pesticides listed in Table 1. The soil samples are treated identically with the exception that the acetone extraction is performed in an acidic environment. HPLC is performed on a Perkin-Elmer Series 200 system equipped with an Altima column (C18, 5 m, (250 x 2.1 mm). Elution is performed in gradient mode (Solvent A: water + 10 mM ammoniumformiate + 0.1% formic acid; Solvent B: ACN + 0.1% formic acid). Liquid flow rate is 200 l.min.-1 Injection volume is 10 l. Mass spectrometry is performed on an API 2000TM LC/MS/MS System, using TurboIonSp
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Simultaneous Determination of Residues of Approximately 100 Pesticides and Metabolites in Fruit and Vegetables by LC/MS/MS
2. Determination of Polar Organophosphorus Pesticides in Aqueous Samples by Direct Injection Using HPLC/MS/MS
3. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. Map and Link Human Genetic Disorders with SSLP Analysis
6. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
7. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
8. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
9. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
10. Increased Sensitivity in Microarray Analysis
11. Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Fast Multi Residue Pesticide Analysis Soil and Vegetable Samples

(Date:7/31/2014)... July 31, 2014  Smith & Nephew (LSE:SN, ... technology business, today announced its entry into the ... Lesser Toe Repair System. Comprised of three separate ... metatarsophalangeal (MTP) ligament repair and reconstruction, a metatarsal ... proximal Inter-phalanges (PIP) fusion, also known as hammer ...
(Date:7/31/2014)... 31, 2014 The global market for ... by 2020, according to a new study by Grand ... theranostics, and the subsequent introduction of advanced cancer diagnostic ... growth over the next six years. Moreover, the growing ... as cancer, coupled with disease triggering lifestyle habits such ...
(Date:7/31/2014)... 31, 2014 SoundConnect , ... company, is proud to announce the addition of ... Adobe Connect’s elearning tools to quickly deploy and ... with training tools for desktop and mobile learning ... is the perfect online training solution, enabling organizations ...
(Date:7/30/2014)... Regulus Therapeutics Inc . (NASDAQ: ... and development of innovative medicines targeting microRNAs, today announced ... the quarter ended June 30, 2014 on Wednesday, August ... Regulus will host a conference call and ... Eastern Daylight Time to discuss its second quarter 2014 ...
Breaking Biology Technology:Smith & Nephew scores a HAT-TRICK with its entry into the high-growth hammer toe repair market 2Smith & Nephew scores a HAT-TRICK with its entry into the high-growth hammer toe repair market 3Smith & Nephew scores a HAT-TRICK with its entry into the high-growth hammer toe repair market 4Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 2Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 3Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 4Molecular Diagnostics Market - Global Industry Size, Share, Trends, Analysis, Players, Opportunity, Outlook and Forecast From 2014 to 2020 5SoundConnect Enhances Portfolio with Adobe Connect Learning Management System (LMS) 2Regulus Announces Timing for Second Quarter 2014 Financial Results Webcast and Conference Call 2Regulus Announces Timing for Second Quarter 2014 Financial Results Webcast and Conference Call 3
... Inc. (Nasdaq:,NUVA), a medical device company focused on developing ... today,details relating to its fourth quarter and full year ... 19, 2008, after,the close of the market., NuVasive ... 2008 at,5:30 p.m. ET / 2:30 p.m. PT to ...
... of Directors of US,Oncology Holdings, Inc. and its wholly ... "Company") announced that, effective,February 1, 2008, Dale Ross will ... and will continue as the Executive Chairman of the,Company. ... Company,s,President and CEO. The CEO transition is a planned ...
... investors to state, TOPEKA, Kan., Jan. 25 ... a "Come Home to Kansas" initiative that,focuses on ... Kansas. Partners in this project include the Kansas,Department ... Organization and the KTEC PIPELINE., This initiative ...
Cached Biology Technology:NuVasive Announces Webcast and Conference Call of Fourth Quarter and Full Year 2007 Results 2NuVasive Announces Webcast and Conference Call of Fourth Quarter and Full Year 2007 Results 3US Oncology Announces Realignment of Executive Team 2US Oncology Announces Realignment of Executive Team 3'Come Home to Kansas' Announced by KTEC 2
(Date:7/31/2014)... A first-of-its-kind study of bigeye tuna movements in the ... the Large Pelagics Research Center at the University of ... cover a wide geographical range with pronounced north-south movements ... favor a high-use area off Cape Hatteras southwest of ... a new approach to study one of the most ...
(Date:7/31/2014)... GAINESVILLE, Fla. --- By tracing nearly 3,000 genes ... moths, University of Florida scientists have created an ... to use large-scale, next-generation DNA sequencing. , ... more closely related to small moths than to ... how butterflies evolved. The study also found that ...
(Date:7/31/2014)... has long been known that biomass burning ... as a ritual , slash-and-burn agriculture and wildfires ... health. , But until the release of a ... Engineering Professor Mark Z. Jacobson, the degree of ... Jacobson,s research, detailed in a paper published July ...
Breaking Biology News(10 mins):Study of bigeye tuna in Northwest Atlantic uses new tracking methods 2Study of bigeye tuna in Northwest Atlantic uses new tracking methods 3UF study advances 'DNA revolution,' tells butterflies' evolutionary history 2UF study advances 'DNA revolution,' tells butterflies' evolutionary history 3Stanford professor finds that wildfires and other burns play bigger role in climate change 2Stanford professor finds that wildfires and other burns play bigger role in climate change 3Stanford professor finds that wildfires and other burns play bigger role in climate change 4
... headed by Prof. Johanna Ivaska (University of Turku and VTT ... of Turku and the National Institute for Health and Welfare) ... Published in a leading journal, the study concludes ... and of lung and prostate cancer cells. It is likely ...
... scientists have developed a new, integrated, ten-year science plan to ... role in it. Understanding the carbon cycle is central for ... builds on the first such plan, published in 1999, but ... as agents and managers of carbon cycling and climate change, ...
... COPENHAGEN (16 November 2011) In the lead up ... this month, an independent global commission of eminent scientists today ... how to achieve food security in the face of climate ... commissioners urged immediate, coordinated action toward transforming the food system ...
Cached Biology News:Scientists tackle the carbon conundrum 2Global commission charts pathway for achieving food security in face of climate change 2Global commission charts pathway for achieving food security in face of climate change 3Global commission charts pathway for achieving food security in face of climate change 4Global commission charts pathway for achieving food security in face of climate change 5Global commission charts pathway for achieving food security in face of climate change 6Global commission charts pathway for achieving food security in face of climate change 7
Human peripheral blood CD19+ B cells, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Monocytes, B Cells, NK Cells and Stem Cells...
Umbilical cord blood CD14+ Monocytes, For immunohistochemistry (IHC) Cell Chip with Human Umbilical Cord Blood Monocytes, B Cells and NK Cells...
Umbilical cord blood basophils, For immunohistochemistry (IHC)...
Human peripheral blood neutrophils, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Granulocytes...
Biology Products: