( or higher than this number indicatin...)Fast Isolation of Total RNA from Small Numbers of Cells

or higher than this number, indicating high yields. The A₂₆₀/A₂₈₀ ratios measured for all samples isolated from 5 x 10⁵ or 1 x 10⁵ cells were 2.0 to 2.1, indicating high-quality RNA.

Average Yields of RNA* from Smaller Numbers of Cells

Cell line

10⁴ cells

10³ cells

10² cells

10¹ cells

Yields (ng)

HeLa

243

28

ND**

0.4

HL-60

86

8.2

0.8

0.2

THP-1

88

10.3

0.8

0.3

NIH/3T3

176

17.0

1.1

1.0

CHO

154

12.5

1.4

0.5

* RNA was quantitated by RiboGreen assay. Isolations were performed in duplicate.
** Not done

Total RNA from smaller samples (Tab
'"/>

Source:

Page: All 1 2 3 4 5 6
Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
5. Isolation of Microorganisms
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Basic Plasmid DNA Isolation Protocol
10. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
11. RNA-free Plasmid DNA Isolation Protocol