( ssary buffers and components for total ...)Fast Isolation of Total RNA from Small Numbers of Cells

ssary buffers and components for total RNA purification including lyophilized DNase I for convenient room temperature storage.

Isolating RNA from Different Sources

Average yields of RNA from various cells

Cell line

5 x 10⁵ cells

1 x 10⁵ cells

Yield (g)

A₂₆₀/A₂₈₀

Yield (g)

A₂₆₀/A₂₈₀

HeLa

10.1

2.0

2.1

2.0

HL-60

2.8

2.1

0.5

2.1

THP-1

4.1

2.0

0.7

2.1

NIH/3T3

7.3

2.0

1.5

2.1

CHO

5.7

2.0

1.2

2.1

Table 1 and Table 2 show total RNA yields from varying cell numbers of different cell types. A typical mammalian cell contains approximately 10^-5 g of total RNA.¹ The yields for all cell lines are close to
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