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FREE FAT CELL ASSAY

ved to be comparable to those from using the toluene scintillator extraction system. Using the same batch of cells for comparing the two extraction systems gave ED50 of 103 10 pM for toluene scintillator and 97 7 pM for MicroScint-E. The full dose response curves are shown in Figure 1.

Conclusion

The MicroScint-E scintillator from Packard Instrument Company provides the characteristics necessary to extract and count free fat cell assay samples in 96-well microplates in the TopCount without physically removing the aqueous phase. This heterogeneous counting method simplifies the assay, and enables rapid preparation and multiple-detector counting of such assays. MicroScint-E also eliminates the hazardous properties of toluene scintillator system, which was formerly the extractant of choice.

References

1) Brange J, Ribel U, Hansen JF, Havelund S, Melberg SG, Norris F, Norris K, Snel L, Srensen AR, Voigt HO (1988). Nature, 333, 679-682.

2) Vlund Aa, Brange J, Drejer K, Jensen I, Markussen J, Ribel U, Srensen AR, Schlichtkrull J. Diabetic Medicine 1991; 8: 839-847.

3) Martin Rodbell. (1964). The Journal of Biological Chemistry. 239(2).375-380.

4) A. J. Moody, M. A. Stan, M. Stan. (1974), Horm.Metab.Res.6.12-16.


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