Navigation Links
FREE FAT CELL ASSAY

n per sample is incubated with 10 μL glucose solution and 10 μL insulin for two hours at 37 C using gentle shaking. The incubation is stopped by adding 150 μL of MicroScint-E scintillator, and counted.

Materials

Collagenase is obtained from Wellington, radio labelled D-3-3H-glucose is from Amersham Pharmacia Biotech, and human serum albumin is from Behring Diagnostics GmbH. MicroScint-E is obtained from Packard Instrument Company. All other chemicals are analytical grade or higher.

Buffers:

Weltmans Reagent: 66.2 g CaCl22 H2O, Water ad 1000 mL.

Krebs Stock: 32.26 g NaCl, 1.77 g KCl, 0.811 g KH2PO4, 1.469 g MgSO47H2O, 28 mL Weltmans Reagent, water ad 1000 mL.

10% Human Serum Albumin (HSA): 52.5 g HSA is dissolved in 250 mL water. Add 26.25 g activated charcoal and let it stand for at least 4 hours (room temperature). Shake occasionally. Filter mixture to remove charcoal and dialyse to remove ions. Dilute to 500 mL.

Hepes pH 7.9: 11.92 g Hepes is dissolved in 50 mL water. Adjust pH to 7.9 using 1N NaOH. Dilute to 100 mL. Hepes Buffer: 40 mL Krebs, 50 mL 10% HSA, 10 mL 0.5M Hepes pH 7.9 Water ad 200 mL.

Incubation Buffer: 100 mL Krebs, 50 mL 10% HSA, 25 mL Hepes pH 7.9, water ad 500 mL.

Degradation Buffer: 4 mg collagenase, 2 mg glucose, 4 mL 10 % HSA, 2 mL Krebs, 0.5 mL Hepes pH 7.9, 3.5 mL water.

Glucose Solution: 12.5 μL D(3H3) glucose 1 mg glucose 1 mL Hepes buffer.

Results

Extraction of only the lipids is crucial for the interpretation of the insulin action, and the results from MicroScint-E extraction pro
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. DELFIA Protein Kinase assays.
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
4. Organize Your Data from the Big Blue Mutation Assay
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
7. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Using Validated siRNAs in Functional Genomic Assays
10. Nuclease Protection Assays: The Basics
11. RNArticles: RNA Amplification Used to Develop a New In Vitro HCMV Assay
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FREE FAT CELL ASSAY

(Date:9/19/2014)... 19, 2014 , The first ... for Paediatric Endocrinology (ESPE) Meeting  Investment supports ... research in the field of growth  , ... Germany , today announced the first recipients ... The awards were announced during a Satellite Symposium organized ...
(Date:9/18/2014)... September 18, 2014 HealthTronics, ... and interventional radiology products and services, announced that ... cryotherapy and cryoablation) versus external beam radiotherapy (EBRT) ... the American Urological Association (MAAUA) Annual Meeting in ... Relative Effectiveness of Cryosurgery and External Beam Radiation ...
(Date:9/18/2014)... 2014 A novel robotic system that ... is currently being tested as part of a biomedical ... Boston with the aim of determining if the robot, ... cancer biopsies faster, more accurate, less costly, and less ... the potential to deliver prostate cancer therapies with greater ...
(Date:9/18/2014)... Texas (PRWEB) September 18, 2014 ... Chancellor John Sharp, Texas A&M Health Science Center ... U.S. Department of Health and Human Services (HHS), ... dedicated a national pandemic influenza vaccine manufacturing facility ... as an anchor for the Texas A&M Biocorridor ...
Breaking Biology Technology:EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 2EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 3HealthTronics Announces Data Comparing Cryotherapy Versus Radiation for Treating Prostate Cancer Poster Presented at the American Urological Association Regional Meeting 2Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 2Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 4Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 5Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 6Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 2Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 3Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 4
... -- Cingular Wireless will invest more than $300 ... Wisconsin -- including turning on-air 110 new cell sites, ... will spend $6.2 billion to enhance and expand its ... year, Cingular put into service 22 cell ...
... Attorney Joe Hildebrandt has been a part of ... some close at home and others in the financial ... the years, Hildebrandt told a legislative committee last week, ... state" that neither wants nor welcomes investors. , ,Lately, ...
... Wis. - Milton Silva-Craig has joined the board ... reported on Monday. , ,Neurognostics, which spun out of ... resonance imaging (fMRI) products and services , ,Silva-Craig is ... which he joined in March, 2001. Emageon ...
Cached Biology Technology:State's investment in early-stage businesses is changing perceptions 2State's investment in early-stage businesses is changing perceptions 3
(Date:9/18/2014)... A novel robotic system that can operate inside ... tested as part of a biomedical research partnership program ... aim of determining if the robot, in conjunction with ... more accurate, less costly, and less discomforting for the ... deliver prostate cancer therapies with greater precision. , Developed ...
(Date:9/18/2014)... starts a "frequent flyers" program, fruit flies surely would ... has hosted increasing numbers of fruit fly research studies. ... in April, and another is scheduled launch to the ... launch in December. , Fruit flies are biomedical research ... space. Model organisms can reveal the basis for health ...
(Date:9/18/2014)... foliage season that prompts millions of Americans to undertake jaunts ... possibly last a little longer within a century, according to ... in some areas of the United States as summer temperatures ... the journal Global Ecology and Biogeography . For instance, ... the state tree of New Hampshire could change color ...
Breaking Biology News(10 mins):Trial begins for MRI-compatible robot designed to improve accuracy of prostate biopsies 2Trial begins for MRI-compatible robot designed to improve accuracy of prostate biopsies 3Trial begins for MRI-compatible robot designed to improve accuracy of prostate biopsies 4Trial begins for MRI-compatible robot designed to improve accuracy of prostate biopsies 5Buzzing with activity: Fruit flies orbit Earth for science 2Buzzing with activity: Fruit flies orbit Earth for science 3Buzzing with activity: Fruit flies orbit Earth for science 4Fall foliage season may be later, but longer on warmer Earth 2Fall foliage season may be later, but longer on warmer Earth 3Fall foliage season may be later, but longer on warmer Earth 4
... at the University of South Florida (USF) College of Public ... rodent model that the earliest form of malaria parasites can ... recover, following treatment with the antimalarial drug artesunate. The ... PLoS ONE , suggests that this early-stage dormancy phenomenon ...
... 25, 2011 DST Health Solutions ... Community Health Plan (CCHP) has implemented its PowerMHC® ... Suite to help meet new market requirements and enhance ... implement a nurse assist line service for CCHP, which ...
... is getting even harder to find in the Sierra Nevada, ... the U.S. Forest Service and Oregon State University. A new ... have dropped 60 percent since the 1980sa decrease that may ... which they live, researchers say. Their findings appeared in the ...
Cached Biology News:Dormant malaria parsites in red blood cells may contribute to treatment failure 2DST Health Solutions' Integrated Care Management Suite Improves Care Quality, Population Management of 31,000+ Member Health Plan 2DST Health Solutions' Integrated Care Management Suite Improves Care Quality, Population Management of 31,000+ Member Health Plan 3Fewer marten detections in California forest linked to decline in habitat 2
... your protein is in solution or in a ... mass spectrometry. Proteins will be digested to produce ... of the peptides are determined by MALDI-TOF, and ... peaks (peptides) from MALDI-TOF will be determined by ...
OmniSlide Heated Wash Module. 2 independent chambers with precise temperature control from ambient of 5C to 70C. Each chamber holds 1 slide rack (20 slides). Includes 2 x Wash Sleeves, 1 x Slide R...
For cleavage of 6xHis tags from proteins containing a Factor Xa Protease recognition site Efficient 6xHis tag cleavage (greater than 90%), ...
Request Info...
Biology Products: