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FLIPR Calcium Assay Kit Datasheet

ct transfer of the plates to the FLIPR system for data collection. (See Figure 3.) The elimination of intermediate steps reduces valuable assay time and costs. Also, due to the improved kit chemistry, clear plates may be used instead of black-walled plates to reduce disposable costs significantly.


CONVENIENT PROTOCOL
Another advantage in a high throughput screening campaign is increased convenience. Once dye-loaded with the kit reagents, cells are typically stable for several hours, maximizing assay flexibility. In addition, the FLIPR Calcium Assay Kit has been demonstrated to work equally well with both adherent and non-adherent cells. In many cases, poly-D-lysine coated plates are not required, further lowering the overall assay cost. With the FLIPR Calcium Assay Kit, non-adherent cells are less likely to lift off the plate, and they display greater responsiveness due in part to the elimination of multiple wash/centrifugation steps. Users consistently see equivalent and, in many cases, superior responses to those obtained using the conventional protocol.


ORDERING INFORMATION
For assay development on FLIPR or FLEXstation.

Calcium Assay Kit for FLIPR: Part Number: R8033
(10) reagent vials
(1) buffer bottle
Provides sufficient reagent for 100 plates (96- or 384-well)


Volume discounts given for quantities of 5, 10, and 50 unit orders.

Calcium Assay Kit for FLEXstation: Part Number: R8041
(10) reagent vials
(1) buffer bottle
Provides sufficient reagent for 10 plates (96- or 384-well)


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