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FLIPR Calcium Assay Kit Datasheet

ENABLING HIGH-THROUGHPUT, LOW-COST ASSAYS



NO WASH STEPS

IMPROVED DATA

HIGHER THROUGHPUT

LOW COST


Every major pharmaceutical company uses the Molecular Devices FLIPR fluorometric imaging plate reader system in its drug discovery process. The FLIPR Calcium Assay Kit from Molecular Devices provides the easiest way to get the best performance from your FLIPR system.


OPTIMIZED NO-WASH ASSAY
The FLIPR Calcium Assay Kit was developed and optimized for the FLIPR system to provide a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium. With this kit, calcium assays on FLIPR become a Mix-and-Read procedure in which cells are incubated with the kit reagents for up to one hour and transferred directly to the FLIPR system for evaluation. There are no intermediate wash steps involved.


IMPROVED PHYSIOLOGICAL DATA
The major benefit of the optimized assay is apparent in the quality of the data. The signal obtained with the FLIPR Calcium Assay Kit tends to be higher than that observed in cells loaded with fluo-3 and, at a minimum, comparable to that seen with fluo-4 dye-loaded cells. Furthermore, tighter CVs across 96- and 384-well plates are observed, leading to better results. Figures 1 and 2 show typical data using the FLIPR Calcium Assay Kit.


HIGHER THROUGHPUT, LOWER COST
One advantage in any high throughput screening campaign is the reduced number of steps in the assay, which leads to even higher throughput when used with the FLIPR system. The kits simple procedure involves the direct addition of the kit reagents to the cell plate, incubation for 20 minutes to one hour, and then dire
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