When compared with the standard FLUO-3AM dye incubation-and-wash procedure, assay signal intensity using the FLIPR Calcium 3 Assay Kit is substantially higher and more distinct, resulting in superior detection. Compared to the standard FLUO-4 AM dye incubation-and-wash procedure, the kit produces equivalent or greater signal intensity (See Figure 1) and better well-to-well uniformity resulting in superior data quality and a higher Z-factor. This allows the researcher to accurately and easily detect intracellular calcium fluxes even from chemokine and small peptide targets, as well as transient, endogenous and low-expression receptors that have been difficult to assay with standard methods. Strong signals are attained in cases that may previously have yielded weak signal intensity or no peak at all. Thus, the FLIPR Calcium 3 Assay Kit negates the need for having multiple calcium flux assay chemistries for different receptor targets.
RAPID ASSAY DEVELOPMENT
The streamlined, homogeneous format makes the FLIPR Calcium 3 Assay Kit less labor intensive to run. With the Mix-and-Read procedure, the cells can be incubated with the reagents and are stable for up to several hours. Rapid analysis of the cells can be followed with detection on a FLIPR system or FlexStation microplate reader.
The FLIPR Calcium 3 Assay Kit increases throughput by eliminating cumbersome wash steps. Less preparation time is required for wash buffers and wash calibrations. This successfully eliminates causes for variability in the data, resulting in fewer false positives and negatives. The kit is designed to work at room temperature, which is conducive to automation using stackers or robots. Larger unit volume packaging in the Express Kit format minimizes reagent bottle an