Navigation Links
FLIPR Calcium 3 Assay Kit Datasheet

assay protocol results in fewer spontaneous calcium fluxes and unresponsive cells.

When compared with the standard FLUO-3AM dye incubation-and-wash procedure, assay signal intensity using the FLIPR Calcium 3 Assay Kit is substantially higher and more distinct, resulting in superior detection. Compared to the standard FLUO-4 AM dye incubation-and-wash procedure, the kit produces equivalent or greater signal intensity (See Figure 1) and better well-to-well uniformity resulting in superior data quality and a higher Z-factor. This allows the researcher to accurately and easily detect intracellular calcium fluxes even from chemokine and small peptide targets, as well as transient, endogenous and low-expression receptors that have been difficult to assay with standard methods. Strong signals are attained in cases that may previously have yielded weak signal intensity or no peak at all. Thus, the FLIPR Calcium 3 Assay Kit negates the need for having multiple calcium flux assay chemistries for different receptor targets.


RAPID ASSAY DEVELOPMENT
The streamlined, homogeneous format makes the FLIPR Calcium 3 Assay Kit less labor intensive to run. With the Mix-and-Read procedure, the cells can be incubated with the reagents and are stable for up to several hours. Rapid analysis of the cells can be followed with detection on a FLIPR system or FlexStation microplate reader.


INCREASED THROUGHPUT

The FLIPR Calcium 3 Assay Kit increases throughput by eliminating cumbersome wash steps. Less preparation time is required for wash buffers and wash calibrations. This successfully eliminates causes for variability in the data, resulting in fewer false positives and negatives. The kit is designed to work at room temperature, which is conducive to automation using stackers or robots. Larger unit volume packaging in the Express Kit format minimizes reagent bottle an
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. FLIPR Calcium Assay Kit Datasheet
2. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
3. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Cis-Reporting System Monitors Intracellular Calcium Mobilization
6. Cis-Reporting System Monitors Intracellular Calcium Mobilization
7. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
8. Calcium Flux Assays using the LabChip 3000
9. HEK293 Calcium Flux Assay
10. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
11. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FLIPR Calcium Assay Kit Datasheet

(Date:7/30/2014)... 2014 Adding to their already extensive ... special – Buy 2 of any Weighing Boats ... benefit lab technicians looking for durable and reliable weighing ... for multiple applications including weighing liquids, solid, or granulated ... including diamond-shaped, hexagonal, square, aluminum weighing, and standard boats. ...
(Date:7/29/2014)... Dallas, Texas (PRWEB) July 30, 2014 ... in Private Equity and Venture Capital Funding” report ... factors shaping the private equity and venture capital ... that these are expected to have significant impact ... the US and globally. Furthermore, the report identifies ...
(Date:7/29/2014)... Human Longevity, Inc. (HLI), a genomics and ... the healthy, high performance human life span, today announced ... expert in machine learning and machine translation as the ... Google where he was Distinguished Research Scientist and the ... HLI Co-Founder and CEO, J. Craig Venter , ...
(Date:7/29/2014)... Md. , July 29, 2014  The ... a new and updated program for the 9 ... – 12, 2014 in Amelia Island , ... sites and investigators remains unchanged, however, the topics ... exchange and networking opportunity among all stakeholders with ...
Breaking Biology Technology:Weighing Boat Promotion Now Available at Pipette.com, Major Pipette Distributor 2Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 2Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 3Strategic Trends of Biotech Investment Industry (Private Equity and Venture Capital Funding) in New Report Available at MarketOptimizer.org 4Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 2TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 3
... Australian banks , Brookfield, Wis. Check-processing firm ... banks Commonwealth Bank of Australia, National Australia Bank and ... system. If negotiations are completed successfully, the three banks would ... managed by Fiserv. , Herrera to join Conjugons board of ...
... , CRN magazine ranked Laurie Benson, CEO of Madison-based ... executives in 2004. The magazine looked for influential leaders of ... executives from IBM, Cisco, Intel, Sun and other well-known companies. ... , Milwaukee PKWare s chief scientist, Jim ...
... Engineering Expo. This year, 20 teams will present. Photo courtesy ... the University of Wisconsin's biomedical engineering department will ... into the spotlight on Friday at the Biomedical Engineering Design ... Building , 1550 Engineering Drive, 20 teams of students will ...
Cached Biology Technology:Students will show off biomedical devices to professors and investors 2
(Date:7/29/2014)... world of science, researchers sometimes need to get ... did just that to study the impacts of ... coral reef ecosystems at two remote locations in ... animal tracking and computer simulation, McCauley, an assistant ... Marine Biology, and his colleagues sought to understand ...
(Date:7/29/2014)... to the edge of science, Clment Vidal takes on ... and the End: Does our universe have a beginning ... alone in the universe? What is the role of ... science and committed to philosophical rigor, the book presents ... is not an accident, but may well be the ...
(Date:7/29/2014)... 29, 2014  When it comes to technology, healthcare is usually ... the last. However, when it comes to wearables, healthcare is trying ... for the Internet of Things and its wearable cousins is less ... the healthcare industry, whether it,s in the hospital for a major ... With that in mind, InformationWeek editor Rodney Brown ...
Breaking Biology News(10 mins):Underwater elephants 2Underwater elephants 3Book: 'The Beginning and the End' 2
... Using high-resolution functional MRI (fMRI) imaging in patients with ... Columbia University Medical Center (CUMC) researchers have clarified three ... starts there, and how it spreads. In addition to ... detection of the disease, when drugs may be most ...
... towards new malaria treatments by identifying a way to stop ... Nature Chemistry , they show that blocking the activity of ... prevents mice from showing symptoms and extends their lifespan. The ... potently, and hope to start clinical trials of potential treatments ...
... plants has assembled the largest dated evolutionary tree, using ... evolved specific strategies, such as the seasonal shedding of ... researchers, including University of Minnesota professor Peter Reich, will ... Nature . Early flowering plants are thought ...
Cached Biology News:Study shows Where Alzheimer's starts and how it spreads 2Study shows Where Alzheimer's starts and how it spreads 3Malaria drug target raises hopes for new treatments 2Researchers create largest evolutionary 'timetree' of land plants to investigate traits that permit survival in cold climates 2Researchers create largest evolutionary 'timetree' of land plants to investigate traits that permit survival in cold climates 3
... The Biomek FX provides ... analysis applications. With 96-, ... pipetting heads in either single ... Biomek FX offers maximum speed ...
... Use in any standard fixed-angle microcentrifuge ... g.,Spin- centrifuge tube filters consist ... a centrifuge tube. They filter by ... HPLC sample preparation, removal of cells ...
... The GenomeLab GeXP Genetic Analysis System ... scalable, multiplexed PCR*. The systems patented ... standard multiplexed PCR analysis to just a ... GeXP delivers more genes per reaction and ...
... The Chemicon Detergent-OUT Kit is a simple ... protein solutions. Removes detergents without significant ... the protein solution on the Detergent-OUT Spin ... The Med column is suitable for ...
Biology Products: