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FLIPR Calcium 3 Assay Kit Datasheet







Calcium mobilization assays for screening GPCR targets are preferred techniques in drug discovery because they generate high quality leads. Molecular Devices FLIPR system is the method of choice among pharmaceutical and biotechnology companies for screening calcium flux assays in high throughput. Molecular Devices FlexStationis also commonly used in assay development, target validation and lower throughput applications.

To simplify assay development and optimize performance on both FLIPR and FlexStation, Molecular Devices has developed a series of no-wash assay kits. The combination of proprietary chemistry and a no-wash protocol causes less cellular disruption than traditional methods to generate consistent results and strong signals. FLIPR Assay Kits provide the easiest way to get the best performance from your FLIPR or FlexStation system.

FLIPR Assay Kits from Molecular Devices employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology (U.S. patent number 6,420,183, EPO patent number 0 906 572) is offered to drug discovery and life science researchers exclusively by Molecular Devices, through purchase of FLIPR Assay Kits.

Using the patented quench technology and advances in reagent chemistry, the FLIPR Calcium 3 Assay Kit provides an improvement in signal strength and data quality over previous methods. The assay protocol results in fewer spontaneous calcium fluxes and unresponsive cells.

When compared with the standard FLUO-3AM dye incubation-and-wash procedure, assay signal intensity using the FLIPR Calcium 3 Assay Kit is substantially higher and more distinct, resulting in superior detection. Compared to the standard FLUO-4 AM dye incubation-and-wash procedure, the kit produces equivalent or greater signal intensity (See Figure 1) and better well-to-well uniformity resulting in superior data quality and a higher Z-factor. This allows the researcher to accurately and easily detect intracellular calcium fluxes even from chemokine and small peptide targets, as well as transient, endogenous and low-expression receptors that have been difficult to assay with standard methods. Strong signals are attained in cases that may previously have yielded weak signal intensity or no peak at all. Thus, the FLIPR Calcium 3 Assay Kit negates the need for having multiple calcium flux assay chemistries for different receptor targets.

The streamlined, homogeneous format makes the FLIPR Calcium 3 Assay Kit less labor intensive to run. With the Mix-and-Read procedure, the cells can be incubated with the reagents and are stable for up to several hours. Rapid analysis of the cells can be followed with detection on a FLIPR system or FlexStation microplate reader.


The FLIPR Calcium 3 Assay Kit increases throughput by eliminating cumbersome wash steps. Less preparation time is required for wash buffers and wash calibrations. This successfully eliminates causes for variability in the data, resulting in fewer false positives and negatives. The kit is designed to work at room temperature, which is conducive to automation using stackers or robots. Larger unit volume packaging in the Express Kit format minimizes reagent bottle an d liquid handling.

Cell detachment, lowered cell competency and incomplete dye washing are common problems that are easily avoided by using the FLIPR Calcium 3 Assay Kit. In addition, the elimination of potential dispensing and washing errors, or associated equipment failures, further insures the integrity of screening operations.

FLIPR Calcium 3 Assay Explorer Kit: Part Number: R8091
(10) 1 ml reagent vials
(1) buffer bottle
Provides sufficient reagent for 10 plates (96- or 384-well)

FLIPR Calcium 3 Assay Bulk Kit: Part Number: R8090
(10) 10 ml reagent vials
Provides sufficient reagent for 100 plates (96- or 384-well)

FLIPR Calcium 3 Assay Express Kit: Part Number: R8108
(2) 50 ml reagent vials
Provides sufficient reagent for 100 plates (96- or 384-well)

USA 800-635-5577
UK +44-118-944-8000
Germany +49-89-9620-2340
Japan +06-6399-8211

Check our web site for a current listing of our worldwide distributors.

FLIPR is a registered trademark and FLEXstation is a trademark of Molecular Devices Corporation.

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