Navigation Links
FLIPR Calcium 3 Assay Kit Datasheet







Calcium mobilization assays for screening GPCR targets are preferred techniques in drug discovery because they generate high quality leads. Molecular Devices FLIPR system is the method of choice among pharmaceutical and biotechnology companies for screening calcium flux assays in high throughput. Molecular Devices FlexStationis also commonly used in assay development, target validation and lower throughput applications.

To simplify assay development and optimize performance on both FLIPR and FlexStation, Molecular Devices has developed a series of no-wash assay kits. The combination of proprietary chemistry and a no-wash protocol causes less cellular disruption than traditional methods to generate consistent results and strong signals. FLIPR Assay Kits provide the easiest way to get the best performance from your FLIPR or FlexStation system.

FLIPR Assay Kits from Molecular Devices employ a quenching dye to reduce background fluorescence and improve the signal-to-noise ratio. The patented quench technology (U.S. patent number 6,420,183, EPO patent number 0 906 572) is offered to drug discovery and life science researchers exclusively by Molecular Devices, through purchase of FLIPR Assay Kits.

Using the patented quench technology and advances in reagent chemistry, the FLIPR Calcium 3 Assay Kit provides an improvement in signal strength and data quality over previous methods. The assay protocol results in fewer spontaneous calcium fluxes and unresponsive cells.

When compared with the standard FLUO-3AM dye incubation-and-wash procedure, assay signal intensity using the FLIPR Calcium 3 Assay Kit is substantially higher and more distinct, resulting in superior detection. Compared to the standard FLUO-4 AM dye incubation-and-wash procedure, the kit produces equivalent or greater signal intensity (See Figure 1) and better well-to-well uniformity resulting in superior data quality and a higher Z-factor. This allows the researcher to accurately and easily detect intracellular calcium fluxes even from chemokine and small peptide targets, as well as transient, endogenous and low-expression receptors that have been difficult to assay with standard methods. Strong signals are attained in cases that may previously have yielded weak signal intensity or no peak at all. Thus, the FLIPR Calcium 3 Assay Kit negates the need for having multiple calcium flux assay chemistries for different receptor targets.

The streamlined, homogeneous format makes the FLIPR Calcium 3 Assay Kit less labor intensive to run. With the Mix-and-Read procedure, the cells can be incubated with the reagents and are stable for up to several hours. Rapid analysis of the cells can be followed with detection on a FLIPR system or FlexStation microplate reader.


The FLIPR Calcium 3 Assay Kit increases throughput by eliminating cumbersome wash steps. Less preparation time is required for wash buffers and wash calibrations. This successfully eliminates causes for variability in the data, resulting in fewer false positives and negatives. The kit is designed to work at room temperature, which is conducive to automation using stackers or robots. Larger unit volume packaging in the Express Kit format minimizes reagent bottle an d liquid handling.

Cell detachment, lowered cell competency and incomplete dye washing are common problems that are easily avoided by using the FLIPR Calcium 3 Assay Kit. In addition, the elimination of potential dispensing and washing errors, or associated equipment failures, further insures the integrity of screening operations.

FLIPR Calcium 3 Assay Explorer Kit: Part Number: R8091
(10) 1 ml reagent vials
(1) buffer bottle
Provides sufficient reagent for 10 plates (96- or 384-well)

FLIPR Calcium 3 Assay Bulk Kit: Part Number: R8090
(10) 10 ml reagent vials
Provides sufficient reagent for 100 plates (96- or 384-well)

FLIPR Calcium 3 Assay Express Kit: Part Number: R8108
(2) 50 ml reagent vials
Provides sufficient reagent for 100 plates (96- or 384-well)

USA 800-635-5577
UK +44-118-944-8000
Germany +49-89-9620-2340
Japan +06-6399-8211

Check our web site for a current listing of our worldwide distributors.

FLIPR is a registered trademark and FLEXstation is a trademark of Molecular Devices Corporation.

back to top



Page: All 1 2 3

Related biology technology :

1. FLIPR Calcium Assay Kit Datasheet
2. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
3. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Cis-Reporting System Monitors Intracellular Calcium Mobilization
6. Cis-Reporting System Monitors Intracellular Calcium Mobilization
7. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
8. Calcium Flux Assays using the LabChip 3000
9. HEK293 Calcium Flux Assay
10. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
11. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
Post Your Comments:

(Date:10/8/2015)... , Oct. 8, 2015  Genetic testing for mutations ... the identification of more couples at risk of having ... presented today at the 2015 American Society of Human ... in Baltimore, Maryland . The study ... (NYSE: DGX ) are presenting at the conference ...
(Date:10/8/2015)... DURHAM, N.C. and WAYNE, Pa. ... leading management and technology consulting firm, has announced the ... Pennsylvania , majoring in a ... awarded over a two-year period. In addition, the selected ... industry through mentoring with Clarkston Consulting and participation in ...
(Date:10/8/2015)... BALTIMORE, Maryland , October 8, 2015 /PRNewswire/ ... .   --> Goldman ... specializing in the small cap and microcap sectors, ... sponsored research report on PharmaCyte Biotech, Inc. (OTCQB ... company focused on developing and preparing treatments for ...
(Date:10/8/2015)... , Oct. 8, 2015 The 2015 Nobel Prize ... University of Texas at Dallas. Aziz Sancar , who ... Dallas in 1977, is one of three scientists ... continuously monitor and repair damaged DNA on a molecular level.  ... and cell biology from UT Dallas in ...
Breaking Biology Technology:
... Madison, Wis. Paragon Development Systems (PDS) ... expand its presence in Madison into a new building. , ... Madison designs, builds and manages IT infrastructure and services ... vice president of IT infrastructure services, is responsible for restructuring ...
... and undergraduate degree were minted at the University of ... North American operations, the company said on Wednesday. He ... headquartered in Massachusetts, provides "enterprise service bus" technology to ... Wisconsin Department of Administraiton. , ,Though its definition is ...
... Madison, Wis. Massachusetts-based Genzyme Corp. said ... International , a University of Wisconsin-Madison biotech spinoff, for $600 ... company with annual revenues of more than $2 billion and ... the third quarter. , ,Both companies recently reported healthy quarterly ...
Cached Biology Technology:
(Date:9/30/2015)... 30, 2015  The global glucose monitoring device and diabetes ... says a new report on the industry from Kalorama Information. Sales ... the market, followed by continuous glucose monitoring and sensor segment, ... market for these products in its latest report, The ... , ...
(Date:9/28/2015)... NEW YORK , Sept. 28, 2015 ... platform, announced today that its expedited traveler ...  CLEAR,s innovative platform transforms travel, bringing a ... for its members. "CLEAR offers ... which enhances customer service," said Jim ...
(Date:9/28/2015)... September 28, 2015 According to ... & Software), Product (Scanner & Others), Application (Access Control ... & Others) & Geography Global - Forecast to 2020", published ... reach USD 3627.90 Million by 2020, at a CAGR ... Browse 65 market data T ables and ...
Breaking Biology News(10 mins):
... tomato breeders and ketchup fans something to cheer about, ... colleagues at the Hebrew University in Israel have identified ... increase yield. The yield-boosting power of this gene, which ... of tomato, and crucially, across a range of environmental ...
... - A new minimally invasive surgery to correct a ... -- was demonstrated Friday at an international conference attended by ... Germany, Spain and Russia. Technically known as pectus carinatum, ... the patient,s chest the appearance of the breast of a ...
... how a new research program may shed light on how ... subject of a public event that will discuss the findings ... Influence on Human Evolution. Several members of the committee ... be carried out in the next 10 to 20 years ...
Cached Biology News:
Mouse monoclonal [7E10] to C Peptide ( Abpromise for all tested applications). entrezGeneID: 3630 SwissProtID: P01308...
... peptide transporter 2 (APT2) ... transporter PSF2) (Peptide supply ... transporter involved in antigen ... Antigen: ...
... Nabeta2. The epitope specific for ... any other known proteins.,SPECIES REACTIVITIES: ... work on human because the ... (17/19 residues). Reactivity with other ...
Biology Products: