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FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

changes in intracellular calcium following GPCR activation. FLIPR Calcium 3 Assay Kit (Calcium 3), the newest of the no-wash formulation, outperforms its predecessors and offers sensitivity at least equivalent to FLUO-4, and is broadly applicable across a wide array of GPCR targets.

In the examples shown here where data were acquired with sub-optimal transient transfection conditions, the benefit of the no-wash feature of Calcium 3 is highlighted over the use of a conventional dye loading and wash protocol. Data obtained with Calcium 3 were subject to less variability, higher signal dynamic range and elimination of a significant dilution artifact observed with the conventional dye loading and wash protocol. Calcium 3, a sensitive and reproducible indicator for intracellular calcium, is a significant addition to the reagent assortment available to investigators.


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