Navigation Links
FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

n, under optimal cell seeding conditions Calcium 3 yielded greater sensitivity and less variability than FLUO-4, although both reported robust calcium transients. (See Figure 1, inset.)


Figures 2-4 present results from similar comparisons between Calcium 3 and FLUO-4 for the 5-HT1A, 5-HT6 and 5-HT7 receptors transiently expressed in HEK cells. Calcium signaling was facilitated with the 5-HT1A and 5HT6 receptors by co-expression with a chimeric G-protein, and the 5-HT7 receptor was coexpressed with a promiscuous G-protein. Under optimal experimental conditions responses in the range 6000-7000, 5000-6000 and 2000-4000 have been measured with FLUO-4 for 5-HT1A (Figure 2, inset), 5-HT6 and 5-HT7 (not shown) transient expression, respectively. In the 5-HT1A example (Figure 2), use of Calcium 3 resulted in an improved assay performance similar to that described above for the 5-HT2C receptor, i.e., specifically enhanced signal, less variability and reduced dilution artifact. Under optimal conditions, Calcium 3 and FLUO-4 exhibited equivalent performance both in terms of signal range and variability (Figure 2, inset). In the case of the 5-HT6 (Figure 3) and 5-HT7 (Figure 4) receptor evaluations, the difference in assay performance attributable to use of Calcium 3 following non-optimal cell seeding conditions was much more dramatic. Robust calcium transients were measured in the assays run with Calcium 3, while profiles obtained with FLUO-4 were subject to a large dilution artifact and small signal range in response to agonist stimulation.


SUMMARY
A number of fluorescent indicator dyes, including FURA-2, FLUO-3, FLUO-4 and the no-wash reagent FLIPR Calcium and Calcium Plus assay kits, are available for the measurement of
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. FLIPR Calcium Assay Kit Datasheet
2. FLIPR Calcium 3 Assay Kit Datasheet
3. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Cis-Reporting System Monitors Intracellular Calcium Mobilization
6. Cis-Reporting System Monitors Intracellular Calcium Mobilization
7. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
8. Calcium Flux Assays using the LabChip 3000
9. HEK293 Calcium Flux Assay
10. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
11. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
Post Your Comments:
(Date:8/19/2014)... Shimadzu Scientific Instruments has introduced two ... adding to the company’s extensive line-up of HPLC ... operating environment, and full automation, the i-Series provides ... conventional to ultra-high-speed analysis. , The ... so users can begin building the lab of ...
(Date:8/19/2014)... (PRWEB) August 19, 2014 ... to raise $200,000 to fund a corporate lab ... , This development is a critical component ... of care to the implementation of personalized medicine ... of practicing medicine. , Indiegogo contributions will support ...
(Date:8/19/2014)... August 19, 2014 XenoTech announces ... and cost-effective metabolite production from bioactive small molecules. ... platform, the technology enables convenient and timely assessment ... to XenoTech’s Vice President of Commercial Operations, Christian ... assessing clinical risk can be measured directly by ...
(Date:8/19/2014)... 2012, the European Union (EU) mandated use of the ICH-recommended ... – responding to the increasing need to understand and manage ... many regions, the main objectives of the PBRER are to:, ... of new or emerging information on the risks of a ... potential benefits of a drug; and , Allow ...
Breaking Biology Technology:Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 3Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 2Cellgen Diagnostics Launches Indiegogo Crowdfunding Campaign for DNA Medical Testing Device 3Beyond Metabolite Identification, Testable Quantities Now Offered by XenoTech 2Beyond Metabolite Identification, Testable Quantities Now Offered by XenoTech 3Top Five Best Practices for Developing Your Periodic Benefit Risk Evaluation Report (PBRER), New Webinar Hosted by Xtalks 2
... Washington, D.C. - With the opening of a ... Gov. Jim Doyle again urged Senators to overturn ... research. , ,During a morning visit to the West Salem home ... Doyle again used the backdrop of a Wisconsin family with a ...
... the banking and payments technology subsidiary of Marshall ... a new cash-management division and the appointment of its ... , ,Kasik will run the division, which will allow ... income statement items, payables, and receivables with full accounting ...
... - The United States Senate, by a vote of 63-37, ... ban on new federal funding for embryonic stem cell research, setting ... short of the 67 votes needed to override a veto, which ... bill, known as the Stem Cell Research Enhancement Act , ...
Cached Biology Technology:Doyle urges Senate to loosen stem cell restrictions 2Doyle urges Senate to loosen stem cell restrictions 3Doyle urges Senate to loosen stem cell restrictions 4Senate approves bill to expand stem cell funding 2
(Date:8/19/2014)... fish purchased at retail seafood counters in 10 ... can expose consumers to unexpectedly high levels of ... falsely present a fish of the same species, ... dangerous mislabeling offense, according to new research by ... labeling of seafood is essential to allow consumers ...
(Date:8/19/2014)... Scientists at the Houston Methodist Research Institute have figured ... using a pipette -- a common laboratory tool that,s ... preliminary test results in a recent issue of the ... "Studying single cells and their unique functions has become ... faculty member Lidong Qin, Ph.D., the project,s principal investigator. ...
(Date:8/19/2014)... Anyone who has ever had a glass of fizzy ... the air. But in a finding with wide industrial ... push some particles down into the liquid as well. ... aerosol droplets, so we were surprised, and fascinated, to ... the same process injected tiny oil droplets into the ...
Breaking Biology News(10 mins):Seafood substitutions can expose consumers to unexpectedly high mercury 2Seafood substitutions can expose consumers to unexpectedly high mercury 3Moving single cells around -- accurately and cheaply 2Bubbling down: Discovery suggests surprising uses for common bubbles 2Bubbling down: Discovery suggests surprising uses for common bubbles 3
... battle to reduce carbon emissions is at the heart ... of Missouri have discovered that nature has been lending ... in the Department of Forestry discovered that trees submerged ... years, a significantly longer period of time than trees ...
... grew wild in Mexico and were radically different from ... important crops in the world. While the ... Mexico, the time and location of the earliest domestication ... Now, in addition to more traditional macrobotanical and archeological ...
... A detailed analysis of data from nearly 50 ... adjacent Rhode Island Sound has revealed a long-term shift ... effects of global warming. According to Jeremy Collie, ... Graduate School of Oceanography, the fish community has shifted ...
Cached Biology News:Ancient oak trees help reduce global warming 2Early origins of maize in Mexico 2Early origins of maize in Mexico 3Climate change causing significant shift in composition of coastal fish communities 2Climate change causing significant shift in composition of coastal fish communities 3
...
BD BaculoGoldTM Transfection Kit 5 transfections...
... Purification Kit is designed for isolating DNA from ... protocol can be completed in less than one ... larger amounts of DNA. The purified DNA can ... Southern blotting. The MasterPure Kit was designed for ...
...
Biology Products: