Navigation Links
FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

ells were loaded with the calcium indicator dye FLUO-4AM reagent for 60 minutes at 37C. Subsequently, cells were washed once with HBSS at room temperature, 180 l HBSS was added to each well and plates were transferred to FLIPR for acquisition of calcium images. Excitation at 488 nm was achieved with an Argon ion laser and a 515 nm emission filter was used. In each experiment baseline fluorescence after dye loading was adjusted to 10,000 fluorescence counts by adjusting the strength of the laser. Fluorescence images and relative intensities were captured at 1 second intervals and cells were stimulated by addition of agonist (20 l) after 10 baseline measurements.

Calcium 3 FLIPR studies
Growth medium was removed by aspiration, 180 l Calcium 3 reagent, reconstituted in HBSS, was added and cells were loaded for 60 minutes at 37C. Subsequently, plates were transferred to FLIPR for acquisition of calcium images.


RESULTS
Calcium responses to a maximally effective concentration of 5-HT (10 M) in HEK cells transiently expressing the 5-HT2C (VSV isoform) receptor measured using either Calcium 3 or FLUO-4 are presented in Figure 1. In all data examples shown, cells were seeded at modest over-confluence at the time of transfection. That the results obtained for the 5-HT2C transfection are sub-optimal is evidenced by data from other experiments where maximum fluorescence counts in the range of 8,000-12,000 can be achieved with FLUO-4 using HEK cells transfected with the same construct under optimum cell seeding conditions. (See Figure 1, inset.) Figure 1 illustrates the advantage of using the Calcium 3 no-wash reagent under sub-optimal experimental conditions yielding a higher maximum signal in response to agonist, lower standard errors and improved well-to-well variability and elimination of the dilution artifact observed with FLUO-4 following agonist addition. In additio
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. FLIPR Calcium Assay Kit Datasheet
2. FLIPR Calcium 3 Assay Kit Datasheet
3. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Cis-Reporting System Monitors Intracellular Calcium Mobilization
6. Cis-Reporting System Monitors Intracellular Calcium Mobilization
7. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
8. Calcium Flux Assays using the LabChip 3000
9. HEK293 Calcium Flux Assay
10. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
11. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
Post Your Comments:
(Date:8/26/2015)... After litigating and negotiating patent infringement ... P2i United States patent RE43,651 (the ... the United States without any admission or ... party. As a result of the parties, settlement, the US ... dismissed the case without prejudice. Under ...
(Date:8/26/2015)... ... August 26, 2015 , ... The 2015 Epigenomics & Metabolomics Meeting ... company Asymmetrex to share the first report on progress with its crowdsourcing ... counting adult tissue stem cells. , With a name like “H2A.Z asymmetry,” the new ...
(Date:8/25/2015)... ... August 25, 2015 , ... Vets ... in Knapp, Wis. The company has added a AS16 Sharples centrifuge to harvest ... collection during bacterial or fungal fermentation process development. , The new centrifuge ...
(Date:8/25/2015)... Diego, CA (PRWEB) , ... ... ... a premier developer of build-to-order, independent blade-based and rackmount computing infrastructure, today ... performance workstation rackmount offering, the RM1630DX. The latest system boasts two distinct, ...
Breaking Biology Technology:P2i Settles United States Litigation 2Asymmetrex Reports Positive Indicators for Crowdsourcing Campaign to Evaluate New Biomarker for Counting Adult Tissue Stem Cells for the First Time 2Asymmetrex Reports Positive Indicators for Crowdsourcing Campaign to Evaluate New Biomarker for Counting Adult Tissue Stem Cells for the First Time 3Asymmetrex Reports Positive Indicators for Crowdsourcing Campaign to Evaluate New Biomarker for Counting Adult Tissue Stem Cells for the First Time 4New centrifuge and fermentor expand product development capacity at Vets Plus 2Cirrascale® Announces Next Generation High Density, Two-in-One GPU Rackmount Offering Perfect for High Performance Remote Workstations 2Cirrascale® Announces Next Generation High Density, Two-in-One GPU Rackmount Offering Perfect for High Performance Remote Workstations 3
... 2011 ,   ... Matthew Roe as Chief Business Officer. Matthew joins,Crescendo ... Development & Licensing. During a 23-year ... management, acquisition, divestment, and sales,and marketing, and saw ...
... below 100 nanometres have numerous applications ranging from ... participates in a intragated FP7 European Project called ... nanoimprinting techniques (NIL), as a core aspect for ... followed are cost reduction, simpler fabrication process and ...
... classes of fundamental particles. Photons, the quanta of light, ... up atomic nuclei belong to the fermions. Bosons and ... level. This difference is expressed in their quantum statistics. ... was postulated, which was dubbed the anyon. In their ...
Cached Biology Technology:Crescendo Biologics Appoints Matthew Roe as Chief Business Officer 2Acrobatics for anyons: New test for elusive fundamental particle proposed 2
(Date:8/4/2015)... 2015 Research ... the addition of the "Digital Forensics ... Sub-Segment (Computer Forensics, Network Forensics, Mobile Device ... Vertical and Region - Global Forecast to ... By Component (Hardware, Software, and Service), ...
(Date:7/31/2015)... SHENZHEN, China , 31 de julio de 2015 ... sobre Genómica (ICG-10,  www.icg-10.org ) del 22 al 25 ... . La conferencia celebra su décimo ... se ha convertido en una de las reuniones anuales ... ,ómicas, y es una de las conferencias científicas más ...
(Date:7/31/2015)... , 31. Juli 2015 Die 10. internationale ... von BGI veranstaltet und findet vom 22. bis ... , statt. Die Konferenz ... ihrem Start 2006 ist die ICG weltweit zu ... geworden. Sie ist eines der dynamischsten, enthusiastischsten und ...
Breaking Biology News(10 mins):Digital Forensics Market - The Market Represents a Compound Annual Growth Rate (CAGR) of 11.3% From 2015 to 2020 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 2Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 3
... 2010 American Academy of Ophthalmology (AAO) - Middle ... a report on beta carotene,s ability to ... diseases. The AAO-MEACO meetingthe world,s largest, most comprehensive ... 19 at McCormick Place, Chicago. 9-cis Beta-Carotene ...
... 2010, Singapore A total of 15 world renowned cognitive ... respective fields at the 6th Decade of the Mind Conference ... 2010. Jointly organised by A*STAR,s Institute of High Performance ... NUS,s Temasek Laboratories, it is the first such conference in ...
... University neurobiologists have created mice that can "smell" light, providing ... the neural basis of olfaction. The work, described this ... for the future study of smell and of complex perception ... traditional methods. "It makes intuitive sense to use odors ...
Cached Biology News:Singapore hosts first 'Decade of the Mind' conference in Asia 2Singapore hosts first 'Decade of the Mind' conference in Asia 3Mice that 'smell' light could help us better understand olfaction 2
Unit Definition: 1 U corresponds to the amount of enzyme which produces 1 mol protons per minute at pH 5.5 and 30C (peptin from citrus peel, Fluka-No. 76280, as substrate)...
Recorder, 6", 7 Day, 1 Pen, 0 to 100°C, 220V, 50/60 Hz, for 4520, Customer Installed...
... Serotec STAR3000 HISTAR detection systems provide ... be used with species specific primary ... tissue specimens. Visualisation is accomplished using ... utilises novel polymer labelling technology. Secondary ...
Taq DNA Polymerase (T. aquaticus), 250 units. For PCR and other procedures requiring DNA polymerase activity at elevated temperatures. Category: Nucleotides & Enzymes & Biochemicals, Modifying Enzyme...
Biology Products: