Navigation Links
FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

ells were loaded with the calcium indicator dye FLUO-4AM reagent for 60 minutes at 37C. Subsequently, cells were washed once with HBSS at room temperature, 180 l HBSS was added to each well and plates were transferred to FLIPR for acquisition of calcium images. Excitation at 488 nm was achieved with an Argon ion laser and a 515 nm emission filter was used. In each experiment baseline fluorescence after dye loading was adjusted to 10,000 fluorescence counts by adjusting the strength of the laser. Fluorescence images and relative intensities were captured at 1 second intervals and cells were stimulated by addition of agonist (20 l) after 10 baseline measurements.

Calcium 3 FLIPR studies
Growth medium was removed by aspiration, 180 l Calcium 3 reagent, reconstituted in HBSS, was added and cells were loaded for 60 minutes at 37C. Subsequently, plates were transferred to FLIPR for acquisition of calcium images.


RESULTS
Calcium responses to a maximally effective concentration of 5-HT (10 M) in HEK cells transiently expressing the 5-HT2C (VSV isoform) receptor measured using either Calcium 3 or FLUO-4 are presented in Figure 1. In all data examples shown, cells were seeded at modest over-confluence at the time of transfection. That the results obtained for the 5-HT2C transfection are sub-optimal is evidenced by data from other experiments where maximum fluorescence counts in the range of 8,000-12,000 can be achieved with FLUO-4 using HEK cells transfected with the same construct under optimum cell seeding conditions. (See Figure 1, inset.) Figure 1 illustrates the advantage of using the Calcium 3 no-wash reagent under sub-optimal experimental conditions yielding a higher maximum signal in response to agonist, lower standard errors and improved well-to-well variability and elimination of the dilution artifact observed with FLUO-4 following agonist addition. In additio
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. FLIPR Calcium Assay Kit Datasheet
2. FLIPR Calcium 3 Assay Kit Datasheet
3. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Cis-Reporting System Monitors Intracellular Calcium Mobilization
6. Cis-Reporting System Monitors Intracellular Calcium Mobilization
7. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
8. Calcium Flux Assays using the LabChip 3000
9. HEK293 Calcium Flux Assay
10. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
11. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
Post Your Comments:
(Date:5/17/2013)... PA (PRWEB) May 17, 2013 Oven ... ramp/soak capabilities. The 5R6-900 temperature controller has many user-friendly ... be plugged into the wall as a self-contained ... supply. This distinctive detail makes the device unique, as ... The controller can also be used universally, which allows ...
(Date:5/17/2013)... 2013 •    First of its kind research ... ,     New facility will help solve crop stresses ... Syngenta unveiled its new crop research facility during ... Innovation Center. The first of its kind, $72 million ... agricultural climate and precisely measure plant inputs – the ...
(Date:5/17/2013)... May 17, 2013  Insero Health, Inc., a company ... epilepsy and related neurological disorders, is today reporting top-line ... compound INS001 in patients with drug-resistant epilepsy.  The data ... Trials (AED) Xll meeting by Dr. Steven ... of Insero,s Scientific Advisory Board.  In this study, INS001 ...
(Date:5/17/2013)... (PRWEB) May 17, 2013 IAC Industries wants ... a start up laboratory needing to set up and furnish ... to a larger facility within a year’s time. How does ... that the laboratory is temporary? What is efficient and cost-effective? ... modular workstations from IAC Industries. The planners at DisperSol determined ...
Breaking Biology Technology:New Temperature Control Products Announced by Oven Industries Inc. 2Syngenta Opens Unique $72 Million Advanced Crop Lab 2Syngenta Opens Unique $72 Million Advanced Crop Lab 3Insero Health Reports Positive Data on Phase I Trial of Novel Therapy for Drug-resistant Epilepsy 2New Downloadable Success Story: “How To Outfit a Dynamic Lab in Flux” 2
... , New app edition includes more than 1,000-color ... the leading provider of scientific, technical and medical information products ... App, for iPad, is now available on the App Store. ... Second Edition . , ,The Gray,s Anatomy for Students App ...
... Jennerex, Inc., a private clinical-stage biotherapeutics company focused ... oncolytic products for cancer, today announced that positive ... to treat patients with metastatic melanoma were published ... "This clinical trial in metastatic ...
... the Biotechnology and Biological Sciences Research Council (BBSRC) have ... an important disease of livestock - after successfully ... today (1 August 2011) in the journal Proceedings ... scientists with the tools to develop vaccines with useful ...
Cached Biology Technology:Gray's Anatomy for Students, World's Bestselling Anatomy Textbooks, Now Available on iPad 2Gray's Anatomy for Students, World's Bestselling Anatomy Textbooks, Now Available on iPad 3Jennerex Reports Positive Mechanistic Proof-of-Concept Clinical Trial Results Using JX-594 to Treat Metastatic Melanoma 2Jennerex Reports Positive Mechanistic Proof-of-Concept Clinical Trial Results Using JX-594 to Treat Metastatic Melanoma 3Jennerex Reports Positive Mechanistic Proof-of-Concept Clinical Trial Results Using JX-594 to Treat Metastatic Melanoma 4Scientists take a step towards developing better vaccines for bluetongue 2Scientists take a step towards developing better vaccines for bluetongue 3
(Date:5/16/2013)... Integrated Biometrics patented LES (Light Emitting ... film transistor camera introduces Sherlock, a fingerprint scanner ... the smallest and lightest form factor available on ... ID FAP 45, Certified scanner is highly efficient ... weight when compared to optical scanners. ...
(Date:5/16/2013)... at sites outside the bone marrow in the ... in Munich now show that a specific type of ... cells. , Balanced hematopoiesis is essential for the function ... place mainly in the liver and the spleen. Later ... this tissue normally serves as the sole source of ...
(Date:5/16/2013)... in Cameroon and the manager of San Diego Zoo ... prestigious Whitley award. The Whitley award is an ... because it celebrates the extraordinary achievements of some of ... Abwe was selected for the award due to his ... and its wildlife inhabitants., HRH The Princess Royal, the ...
Breaking Biology News(10 mins):Herpes infections: Natural killer cells activate hematopoiesis 2Cameroon researcher wins prestigious 'Green Oscar' 2Cameroon researcher wins prestigious 'Green Oscar' 3
... exciting potential benefit of the rapidly accumulating databases of ... between genomic data and clinical medicine. The research, published ... American Journal of Human Genetics, supports the ... medical record (EMR) systems provide a valuable platform for ...
... (April 1, 2010) Van Andel Research ... for the initiation and development of prostate cancer interact ... treatments for the disease. One of the proteins, ... prostate cancer. The other, steroid receptor coactivator-3 (SRC3), ...
... April, ESA,s CryoSat-2 ice satellite has now joined the rest ... Baikonur Cosmodrome in Kazakhstan. The ,space head module, was ... this morning. Although it is only 7 km between the ... over an hour to complete the journey. , The crocodile ...
Cached Biology News:Electronic medical records may accelerate genome-driven diagnoses and treatments 2VARI study could improve treatments for prostate cancer 2CryoSat-2 installed in launch silo 2
NATIVE PORCINE COLIPASE...
Polyclonal Antibody to CRTR-1...
Recognizes NSCL1. The molecular weight of the protein is 14,616 Daltons....
Mouse monoclonal [3G131] to Desmoglein 1 ( Abpromise for all tested applications). entrezGeneID: 1828 SwissProtID: Q02413...
Biology Products: