Navigation Links
FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

ells were loaded with the calcium indicator dye FLUO-4AM reagent for 60 minutes at 37C. Subsequently, cells were washed once with HBSS at room temperature, 180 l HBSS was added to each well and plates were transferred to FLIPR for acquisition of calcium images. Excitation at 488 nm was achieved with an Argon ion laser and a 515 nm emission filter was used. In each experiment baseline fluorescence after dye loading was adjusted to 10,000 fluorescence counts by adjusting the strength of the laser. Fluorescence images and relative intensities were captured at 1 second intervals and cells were stimulated by addition of agonist (20 l) after 10 baseline measurements.

Calcium 3 FLIPR studies
Growth medium was removed by aspiration, 180 l Calcium 3 reagent, reconstituted in HBSS, was added and cells were loaded for 60 minutes at 37C. Subsequently, plates were transferred to FLIPR for acquisition of calcium images.


RESULTS
Calcium responses to a maximally effective concentration of 5-HT (10 M) in HEK cells transiently expressing the 5-HT2C (VSV isoform) receptor measured using either Calcium 3 or FLUO-4 are presented in Figure 1. In all data examples shown, cells were seeded at modest over-confluence at the time of transfection. That the results obtained for the 5-HT2C transfection are sub-optimal is evidenced by data from other experiments where maximum fluorescence counts in the range of 8,000-12,000 can be achieved with FLUO-4 using HEK cells transfected with the same construct under optimum cell seeding conditions. (See Figure 1, inset.) Figure 1 illustrates the advantage of using the Calcium 3 no-wash reagent under sub-optimal experimental conditions yielding a higher maximum signal in response to agonist, lower standard errors and improved well-to-well variability and elimination of the dilution artifact observed with FLUO-4 following agonist addition. In additio
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. FLIPR Calcium Assay Kit Datasheet
2. FLIPR Calcium 3 Assay Kit Datasheet
3. Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR
4. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
5. Cis-Reporting System Monitors Intracellular Calcium Mobilization
6. Cis-Reporting System Monitors Intracellular Calcium Mobilization
7. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
8. Calcium Flux Assays using the LabChip 3000
9. HEK293 Calcium Flux Assay
10. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
11. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
Post Your Comments:
(Date:7/31/2014)... 31, 2014 Senomyx , Inc. ... using proprietary taste science technologies to discover, develop, ... beverage, and ingredient supply industries, today reported financial ... ended the second quarter with $33.6 million in ... "During the past quarter Senomyx achieved another important ...
(Date:7/31/2014)... CA (PRWEB) July 31, 2014 ... today the integration of Omicsoft’s Array Suite with ... platforms will be able to send data in tranSMART ... works with all versions of tranSMART including the new ... art statistics, visualization and storage for the analysis of ...
(Date:7/31/2014)...   BioLife Solutions , Inc. (NASDAQ: ... proprietary clinical grade hypothermic storage and ... products for cells and tissues, today announced today ... be released on Thursday, August 7, 2014, and that ... webcast at 4:30 p.m. ET that afternoon. Management will ...
(Date:7/31/2014)... Scottsdale, AZ (PRWEB) July 31, 2014 ... the availability of its online sales training course – ... salespeople improve their sales performance. , Drawn from proven ... Fortune 500 companies, Mastering the Complex Sale online sales ... as to anyone who has face-to-face contact with customers, ...
Breaking Biology Technology:SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 2SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 3SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 4SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 5SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 6SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 7SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 8SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 9SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 10SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 11SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 12SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 13SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 14SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 15SENOMYX ANNOUNCES CORPORATE UPDATE AND SECOND QUARTER 2014 FINANCIAL RESULTS 16Rancho BioSciences, in Partnership with Omicsoft, have Integrated Array Suite with tranSMART 2Rancho BioSciences, in Partnership with Omicsoft, have Integrated Array Suite with tranSMART 3BioLife Solutions to Report Financial Results for Second Quarter 2014 and Provide Business Update on August 7, 2014 2Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 2Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 3
... MBF Healthcare,Acquisition Corp. (Amex: MBH ), a ... it and Critical Home Solutions,Holdings, Inc., a privately-owned, ... infusion services, are scheduled to present,on Wednesday, February ... in New York, NY. On February 7, 2008, ...
... CITY, Calif., Feb. 12 /PRNewswire-FirstCall/ . Maxygen, Inc.,(Nasdaq: ... the development of,improved protein drugs, today reported financial ... 31, 2007. At December 31, 2007, cash, cash,equivalents ... million at December 31, 2006., "After a ...
... 12 WaferGen Biosystems,Inc. (OTC Bulletin Board: WGBS), ... biology and stem cell research systems,today announced that ... deliver a corporate presentation at the Roth Capital ... will be held,February 18-21, 2008, at the Ritz-Carlton ...
Cached Biology Technology:MBF Healthcare Acquisition Corp. and Critical Homecare Solutions Holdings, Inc. to Present at the 2008 UBS Healthcare Services Conference 2MBF Healthcare Acquisition Corp. and Critical Homecare Solutions Holdings, Inc. to Present at the 2008 UBS Healthcare Services Conference 3Maxygen Reports Fourth Quarter and Year End 2007 Financial Results 2Maxygen Reports Fourth Quarter and Year End 2007 Financial Results 3Maxygen Reports Fourth Quarter and Year End 2007 Financial Results 4Maxygen Reports Fourth Quarter and Year End 2007 Financial Results 5Maxygen Reports Fourth Quarter and Year End 2007 Financial Results 6WaferGen to Present at the Roth Capital Partners 20th Annual OC Growth Stock Conference 2
(Date:7/31/2014)... Alexandre Edmond Becquerel first discovered the photovoltaic effect in ... the power of sunlight for its own purposes. In ... issue of the FASEB Journal , ... the power of sunlight by focusing on a naturally ... throughout evolution. This conservationor persistence over time and across ...
(Date:7/31/2014)... In the same week that the U.S. surgeon general ... cancer, researchers at Montana State University published a paper ... code in every cell responds when exposed to ... of DNA damage by the UV rays found in ... and some degenerative eye diseases. , "Our paper ...
(Date:7/31/2014)... Dr. Oren Tessler, Assistant Professor of Clinical Surgery at ... is part of a team of plastic and reconstructive ... method to screen and select patients for a specific ... patients who underwent this surgery to decompress the nerves ... bonus cosmetic eyelid surgery. The study, which confirms the ...
Breaking Biology News(10 mins):Scientists shine bright new light on how living things capture energy from the sun 2New paper describes how DNA avoids damage from UV light 2New paper describes how DNA avoids damage from UV light 3Surgeons report significant migraine relief from cosmetic eyelid surgery technique 2
... California, Berkeley, virologist and an assistant professor in the Department ... Keck Foundation grant, an award given to innovative young scientists ... totals up to $1 million over five years, will go ... as a tool to study the inner workings of human ...
... to a cause of death but now a study of ... cause of major depression and suicide. The international research ... Institute at The University of Western Ontario and Dr. Hymie ... the first to show that proteins that modify DNA directly ...
... Researchers at the University of California, San Francisco, have identified ... killing parrots and exotic birds for more than 30 years. ... PhD, and Don Ganem, MD, also has developed a diagnostic ... PDD, which will enable veterinarians worldwide to control the spread ...
Cached Biology News:UC Berkeley virologist named Keck Distinguished Young Scholar 2Study identifies changes to DNA in major depression and suicide 2UCSF researchers identify virus behind mysterious parrot disease 2