Navigation Links
FERMENTATION BASICS

catabolized to provide energy for cell maintenance, all the enthalpy associated with the oxidation of the substrate is released as heat. If a biochemical product is formed, the heat evolved lies between the heat released during maintenance and that evolved during active growth. The amount of heat evolved is related to the stoichiometry for growth and product formation, whereas the rate of heat evolution is related to the rate of microbial activity.


2.6. Sterilization
cultures at all stages, from the preliminary culture to the fermentor. A fermentor can be sterilized either by destroying the microorganisms with some lethal agent such as heat, radiation, or a chemical, or by removing the viable microorganisms by a physical procedure such as filtration.

During fermentation the following points must be observed to ensure sterility:
sterility of the culture media
sterility of incoming and outgoing air
appropriate construction of the bioreactor for sterilization and for prevention of contamination during fermentation

STERILIZATION OF THE CULTURE MEDIA
Nutrient media as initially prepared contain a variety of different vegetative cells and spores, derived from the constituents of the culture medium, the water and the vessel. These must be eliminated by a suitable means before inoculation. A number of means are available for sterilization, but in practice heat is the most often used mechanism.

A number of factors influence the success of heat sterilization: the number and type of microorganisms present, the composition of the culture medium, the pH value, and the size of the suspended particles. Vegetative cells are rapidly eliminated at relatively low temperatures such as 60 degrees Celcius for 5-10 minutes, but for destruction
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16

Related biology technology :

1. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
2. E. coli Fermentation Using a BioFlo 110 Bench-Top Fermentor
3. Optimizing Your Benchscale Fermentation Process
4. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
5. Glossary for The BioPharm Guide to Fermentation and Cell Culture
6. Fundamentals of Fermentation: Techniques For Benchtop Fermentors
7. 140-Fold Increase of Protein Achieved in Pichia Fermentation
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: FERMENTATION BASICS

(Date:5/21/2013)... May 21, 2013 As the ... strong push within the industry to deliver better ... Organizations and pharma companies often means turning big ... Microtablets, developed in collaboration with Sensidose AB and ... can bring products to market sooner. The partnership ...
(Date:5/21/2013)... 2013  MacroGenics, Inc. today announced that an ... an Fc-modified chimeric monoclonal antibody (MAb), in patients ... will be presented at the 2013 Annual Meeting ... the Developmental Therapeutics - Immunotherapy Oral Abstract Session ... PM.  The presentation will describe the results of ...
(Date:5/21/2013)... York, NY (PRWEB) May 21, 2013 Bed ... And according to My Cleaning Products, many turn to chemical-based ... because of the health and environmental risks those solutions present, ... with Bed Bug Bully instead. , According to ... bed bugs as they used to be. It shared that ...
(Date:5/21/2013)... Inflamax scientists worked closely with internationally ... the study, which will provide critical insight into ... in the study of allergy treatments, the Environmental ... models. Although both the models are well recognized ... direct comparison between the allergic responses evoked by ...
Breaking Biology Technology:The Development of an Innovative Microtablet Dosage Form, a Success Story: Live Webinar Hosted by Xtalks 2MacroGenics Announces Margetuximab (MGAH22) Phase 1 Data Presentation at ASCO; Initiates Phase 2 Clinical Study to Evaluate Activity of Margetuximab in Patients with Metastatic Breast Cancer 2MacroGenics Announces Margetuximab (MGAH22) Phase 1 Data Presentation at ASCO; Initiates Phase 2 Clinical Study to Evaluate Activity of Margetuximab in Patients with Metastatic Breast Cancer 3New Guide to Kill Bed Bugs Published by My Cleaning Products, Company Enumerates Non-Toxic Means to Clear the Pests 2New Guide to Kill Bed Bugs Published by My Cleaning Products, Company Enumerates Non-Toxic Means to Clear the Pests 3Inflamax Research selected by the Immune Tolerance Network and the National Institutes of Health to conduct a landmark clinical study on the underlying mechanisms of allergic inflammation. 2
... GlaxoSmithKline CEO, described the pharmaceutical group,s results as,"robust" in a ... GSK also announced that it will cease ... goals. , Describing 2008 as a ... make very clear what it would take to really make ...
... BETHESDA, Md., Feb. 5 Micromet, Inc. (Nasdaq: MITI ), ... at the BIO CEO and Investor Conference at the Waldorf-Astoria in New ... presentation will be available on the company,s website at www.micromet-inc.com ., ... Date: Tuesday, February 10, 2009, ...
... type 2 diabetes enters clinical developmentCARLSBAD, Calif., Feb. 5 ... announced today it has initiated a Phase 1 study ... of sodium dependent glucose co-transporter type 2 (SGLT2). ... in the kidney. Decreasing SGLT2 levels promotes glucose ...
Cached Biology Technology:Micromet to Present at the BIO CEO and Investor Conference on February 10, 2009 2Micromet to Present at the BIO CEO and Investor Conference on February 10, 2009 3Micromet to Present at the BIO CEO and Investor Conference on February 10, 2009 4Isis Initiates Phase 1 Clinical Trial of SGLT2 Antisense Drug 2Isis Initiates Phase 1 Clinical Trial of SGLT2 Antisense Drug 3Isis Initiates Phase 1 Clinical Trial of SGLT2 Antisense Drug 4
(Date:5/21/2013)... modifications, which alter the way genes function without ... detected in the blood of pregnant women during ... foretell depression in the weeks after giving birth, ... debilitating. , The findings of the small study ... the journal Molecular Psychiatry . , "Postpartum ...
(Date:5/20/2013)... New research suggests that a compound abundant in the ... death. , By altering a very specific step ... into normal cells that die as scheduled. , One ... process that would cause them to die on a ... study in cells, led by Ohio State University researchers, ...
(Date:5/19/2013)... novel study reports that white men and women of ... (hallux valgus) and lesser toe deformities, including hammer or ... to estimate the heritability of foot disorders in humansappear ... published by Wiley on behalf of the American College ... as many as 60% of older adults have foot ...
Breaking Biology News(10 mins):Genetic predictors of postpartum depression uncovered by Hopkins researchers 2Genetic predictors of postpartum depression uncovered by Hopkins researchers 3The compound in the Mediterranean diet that makes cancer cells 'mortal' 2The compound in the Mediterranean diet that makes cancer cells 'mortal' 3Blame your parents for bunion woes 2
... American Geological Institute (AGI), in conjunction with its ... Needs for the Twenty First Century: The Role ... directions for the next President, his administration, federal ... identifies seven national issues and the role geosciences ...
... spiders are voracious predators and consume a wide range of ... have been proposed for why females eat males before or ... University in Ohio found, in a study published in the ... answer may be simpler than previously thought. Males are more ...
... A Florida State University researcher who uses high-powered computers ... mechanism that gives scientists a better understanding of how ... understanding eventually could lead to the development of new ... an assistant professor in FSU,s Department of Chemistry and ...
Cached Biology News:In spiders, size matters: Small males are more often meals 2Computational biochemist uncovers a molecular clue to evolution 2Computational biochemist uncovers a molecular clue to evolution 3
... ELISA Development kit contains the basic components ... to measure natural and recombinant canine Monocyte ... supernates and serum.1 Each kit contains sufficient ... 96-well plates, provided that the following conditions ...
Mouse monoclonal [LPL4A.1] to Plastin L ( Abpromise for all tested applications). SwissProtID: P13796...
Component of HyperMu™ Insertion Kit...
Recombinant Rhesus Macaque IFN-alpha...
Biology Products: