Navigation Links
Extended data analysis using DeCyder EDA

ysis procedure would have to be changed.

Proteins that behaved in a similar way were nicely clustered by using K-means clustering. We could identify proteins that were differentially expressed between normal and malignant, normal and benign, and benign and malignant samples.

Discriminant analysis showed that a small subset of 13 proteins allowed us to discriminate with 100% accuracy between the known groups. The top-ranked proteins from this analysis are candidates for a noninvasive diagnostic test for cancer. The high-abundance proteins from this list are ideal candidates for such a test, because they offer a high probability of being detected as leakage proteins in plasma. Data sets could be created from the top-ranked proteins to build classifiers for the classification of the unknown samples.

If samples show a huge variation (either inherently biological or from sample preparation), proteins could be missing in one or more spot maps, which would affect the final classification. In such a case, one should use a less restrictive data set (i.e. more proteins) for discriminant analysis.

The most important question was whether and how well unknown samples could be classified using DeCyder EDA. We were able to demonstrate that all spot maps could be classified correctly, except for one. Nine unknown patients were classified (10 spot maps, one patient in duplicate). The incorrectly classified sample was one of the duplicates. This sample and its correctly classified duplicate were from poorly cast gels; they should be rerun.

A final comment must be made on the experimental design. For this type of study (classification of unknown samples), we highly recommend having a balanced design. In our study we had only four patients in the benign group and three in the normal group, but 11 patients in the malignant group (all patients run as duplicates). We should have similar numbers of samples in each of the different kno
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
3. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
4. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
5. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
8. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
9. Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Extended data analysis using DeCyder EDA

(Date:7/29/2014)... Lehigh Acres, FL (PRWEB) July 29, 2014 ... (FIB) ex situ lift out specimen preparation solutions, has ... and Trademark Office for specimen manipulation methods incorporating a ... Patent 8,740,209. This is the second company patent ... Lucille A. Giannuzzi. , These complementary patents ...
(Date:7/28/2014)... Texas (PRWEB) July 28, 2014 ... and China Lubricant Industry” is a professional ... Global Lubricant market. The report introduces Lubricant ... and industry chain structure and industry overview. ... including China’s domestic market as well as ...
(Date:7/28/2014)... 28, 2014 According ... Market by Products (Consumables, Instruments, Services), Methods (Phenotypic, Genotypic), Applications (Pharmaceutical, ... (Detection, Characterization) - Global Forecasts to 2019" ... is estimated at $896.5 Million in 2014 ... by 2019, growing at a CAGR of ...
(Date:7/28/2014)... 28, 2014 It’s a hard truth: Nearly ... to further their post-secondary education are failing to obtain a ... this year. And according to an analysis by The ... veteran students’ uninformed choice of college majors, which leads to ... the study: Some 51.7 percent of post-9/11 veterans using ...
Breaking Biology Technology:EXpressLO LLC Granted Second U.S. Patent for Innovative Specimen Preparation Methods 2Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 2Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 3Lubricant Market & Adhesion Promoter Industry (Globa, China) Analysis Now Available at DeepResearchReports.com 4Microbial Identification Market worth $1,194.1 Million by 2019 2Microbial Identification Market worth $1,194.1 Million by 2019 3Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 2Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 3Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 4
... a recent report that probably went unnoticed outside the ... released figures that show why the rest of ... hotbed for scientific research. The numbers demonstrate that investment ... labs; it's about creating jobs for the 21st century ...
... who has served as in-house counsel for the Wisconsin ... 30 to become chief intellectual property counsel for Virent ... prosecution and intellectual property licensing, will continue to work at ... of time after March 30. At WARF, Kettner drafted license ...
... The angel investor market is considered the spawning ground for ... continued growth in the national angel market, a diversified range ... 2006 Angel Market Analysis , the Center for Venture ... reports that angel investing grew by nearly 11 percent in ...
Cached Biology Technology:Tapping hidden assets: Wisconsin researchers who can create jobs 2Tapping hidden assets: Wisconsin researchers who can create jobs 3Tapping hidden assets: Wisconsin researchers who can create jobs 4National angel investment grew 11 percent in `06 2National angel investment grew 11 percent in `06 3
(Date:7/28/2014)... , a wild relative of the domestic tomato, has ... the labs headed by Professors Neelima Sinha and Julin ... The new genome information may help breeders produce tastier, ... in the journal Nature Genetics , was lead ... Germany. The UC Davis labs carried out work on ...
(Date:7/28/2014)... of the Earth that are high priorities for conservation ... particularly vulnerable, as it has the lowest fraction of ... continent in ,refugia, areas of biological diversity that ... constant during times of great environmental change. The refugia ... and Scotland. , The biggest refugia are in ...
(Date:7/28/2014)... & Behavior Research Foundation today announced the winners ... exceptional clinical and basic research by young scientists ... Grants. The grants enable early career scientists to ... for the prevention, early detection, treatment, and cure ... people. , Six young scientistswinners of the Klerman ...
Breaking Biology News(10 mins):Study finds Europe's habitat and wildlife is vulnerable to climate change 2The Brain & Behavior Research Foundation names winners of Klerman and Freedman Prizes 2The Brain & Behavior Research Foundation names winners of Klerman and Freedman Prizes 3The Brain & Behavior Research Foundation names winners of Klerman and Freedman Prizes 4
... and Women,s Hospital (BWH) announces the launch of the ... division within the Department of Medicine. The Channing Division ... biology-driven approach to define the causes of complex diseases. ... develop new ways to classify and treat those diseases. ...
... of Health have developed a new silk-based stabilizer that, ... up to temperatures of 140 degrees Fahrenheit. ... to keep some vaccines and antibiotics refrigerated, which could ... to third world populations. Vaccines and antibiotics often ...
... When coronary blood vessels are constricted, cardiologists try to ... grid-like implants called stents, which stabilize the veins and ... obliteration. A lesser known fact is that stents can ... This kind of respiratory stenosis, which may be caused ...
Cached Biology News:BWH launches a new research division focused on integrating systems biology and medicine 2Vaccine and antibiotics stabilized so refrigeration is not needed -- NIH study 2Vaccine and antibiotics stabilized so refrigeration is not needed -- NIH study 3Non-slip tracheal implants 2
Size & Applications: 100 ul (2 mM). Recommend to use at 1000X dilutions for cell culture (e.g. Add 1 ul to 1 ml of culture medium.) Storage Temp.: -20C Shelf Life: 6-12 month...
... is chromatographically purified from egg white. The extinction ... product is in 10 mM phosphate-buffered saline, ... albumin, 0.05% sodium azide and 50% glycerol. Avidin ... conjugate is purified by molecular sieving ...
in vitro Translation, Accessory Products...
Request Info...
Biology Products: