Navigation Links
Extended data analysis using DeCyder EDA

consisting of all malignant samples, the other containing all the normal and benign samples (not well separated). The malignant cluster showed three subclusters in partial concordance with the classification from the pathologists (Fig 4).

Question 2: Are there proteins or spots that behave similarly to a given protein or spot?
To answer this question we performed K-means clustering with gap statistics. We found nine clusters, each containing 2–40 different proteins, with very good accuracy (Fig 5).

These proteins are candidates for further analysis. Identifying those that are co-regulated might help to uncover the biological basis for this co-regulation.

Question 3: Are there proteins that might be used for the development of noninvasive tests?
To answer this question we performed a discriminant analysis using the Partial Least Square Search routine as the search method and the Regularized Discriminant Analysis (alpha+gamma 0.7) routine as the evaluation method, with 10-fold cross validation. We found that a subset of 13 proteins allowed discrimination between the known classes with 100% accuracy (Fig 6).

If any of these 13 proteins are highly abundant, they will be good candidates for diagnostic tests.

Question 4: Are there proteins or protein patterns that are characteristic of a biological state?
To answer this question a classifier was calculated using the same method that was applied for feature selection. The confusion matrix from the cross-validation showed no errors for the known samples (Fig 7). The created classifier was then applied to the “Unknown” data set.

The result was one more benign than expected and one less malignant than expected. Looking at the classification results, we found that the only “
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
3. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
4. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
5. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
8. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
9. Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Extended data analysis using DeCyder EDA

(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 p.m. – ... Warrington, Pa. , Details: The Hepatitis B Foundation, ... cure for hepatitis B and improving the quality of life ... on Friday, April 11 at Warrington Country Club in Warrington, ...
(Date:1/14/2014)... pet owners drew up their lists of New Year,s resolutions for ... of my furry companion." Nowadays there are pet salons, dog and ... take the little canine or feline darlings along wherever mommy or ... pricey toys at the pet store. But anyone who has ever ...
(Date:1/14/2014)... Boston, MA (PRWEB) January 14, 2014 iLab ... Michelle Detwiler as the new Director of Product Strategy. In ... as well as iLab sub-teams to guide in the development ... that iLab provides the maximum possible benefit to the scientific ...
(Date:1/14/2014)... ®, provider of RapidResponse ®, a cloud-based supply chain ... S&OP ) service, is proud to be a gold sponsor ... at the Hilton San Diego Bayfront, January 30 – 31, ... , Director of Supply Chain at Amgen, as she presents ...
Breaking Biology Technology:Hepatitis B Foundation to Host Annual Crystal Ball Gala 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... Connecticut and SHENZHEN, China, May 15, 2012 ... that a new Roche GS FLX+ System has been successfully ... to complement the existing array of short read sequencing technologies ... GS FLX+ System, developed by 454 Life Sciences, a Roche ...
... small, external bioreactor holding human cells pumped out an ... in a rat with acute inflammation caused by bacterial ... report from researchers at the University of Pittsburgh School ... The findings were published today in the inaugural issue ...
... worse than a shonky pool table with an unseen groove ... study has found that the same goes at the nano-scale, ... "table" made of the semiconductor gallium arsenide. These tiny ... computing technologies. In a research paper titled "The Impact of ...
Cached Biology Technology:BGI Installs Roche GS FLX+ System to Enhance Long Read Sequencing Capabilities for Genome and Transcriptome Research 2BGI Installs Roche GS FLX+ System to Enhance Long Read Sequencing Capabilities for Genome and Transcriptome Research 3Bio-hybrid device acts as 'thermostat' to control systemic inflammation in sepsis 2You can't play nano-billiards on a bumpy table 2
(Date:4/17/2014)... word for chili peppers. Information about archaeological remains of ... of the appearance of words for chili peppers in ... domesticated and highlight the value of multi-proxy data analysis. ... of nine papers presented in a special feature issue ... Sciences on plant and animal domestication edited by ...
(Date:4/17/2014)... Foundation has been named awardee of a three-year ... Defense Spinal Cord Injury Research Program. Gail Forrest, ... double-blinded, controlled, multi-site clinical trial, which will test ... spinal cord injury. Dr. Forrest is assistant director ... Foundation. Two additional sites will participate - the ...
(Date:4/17/2014)... Professor Marc Andr Gauthier and Professor Luca Razzari ... each been awarded large grants from the John ... for Innovation (CFI) for the acquisition of state-of-the-art ... be added matching grants from the Ministre de ... et de la Technologie (MESRST). These new laboratories ...
Breaking Biology News(10 mins):Chickens to chili peppers 2Chickens to chili peppers 3Kessler Foundation awarded Department of Defense grant for spinal cord injury research 2New state-of-the-art biotech and nanotech equipment for INRS 2
... study at Oregon State University indicates that some past approaches ... the number of live trees that burn up and the ... result. The research was done on the Metolius River ... or 100,000 acres of the area burned in ...
... upspeak: the habit of making a sentence sound like a question? ... as in upspeak may be a sign of superior empathy? ... finds that people use the same brain regions to produce and ... by imitating through so-called mirror neurons. This study shows for the ...
... special edition of the Elsevier journal Ocean Engineering ... an overview of the lessons learned in the aftermath of ... natural disaster in U.S. history. Katrina,s size was larger than ... nearly 93,000 square miles. Katrina,s winds and storm surge overwhelmed ...
Cached Biology News:Effects of forest fire on carbon emissions, climate impacts often overestimated 2Effects of forest fire on carbon emissions, climate impacts often overestimated 3Music in speech equals empathy in heart? 2A forensic analysis of Hurricane Katrina's impact: methods and findings 2
3M Imidazole Solution 125 ml...
PFM8 Antibody...
Goat polyclonal to DDDDK tag (FITC) ( Abpromise for all tested applications)....
Goat polyclonal to Nebraska Calf Diarrhea Virus (FITC) ( Abpromise for all tested applications). Antigen: Bovine (Nebraska Calf Diarrhea Virus)....
Biology Products: