Navigation Links
Extended data analysis using DeCyder EDA

Key words: DeCyder EDA • extended data analysis • workspace • base set • differential expression analysis • principal components analysis • pattern analysis • discriminant analysis • Ettan DIGE


DeCyder™ Extended Data Analysis (EDA) Software was used to perform advanced statistics on samples from a study on human ovarian cancer. The known samples could be clearly separated into normal, benign, and malignant, agreeing with classifications made by pathologists. The classifier created from the known samples was used to classify the unknown samples. All unknown spot maps were classified correctly, except for one from a poorly cast gel. The incorrectly classified sample was a duplicate of a sample that had been correctly classified.


Introduction
The Ettan™ 2-D Difference Gel Electrophoresis (DIGE) system has greatly reduced system variation, to a level that allows the detection of minor expression changes of as little as 10%.

Univariate analysis within DeCyder 2-D Differential Analysis Software (DeCyder 2-D)—Student’s t-test, one-way Analysis Of Variance (ANOVA), and two-way ANOVA—allows the identification of proteins that have altered expression with a given statistical confidence, as well as the interaction of two conditions.

Nevertheless, there might be remaining questions:
• How many groups or classes exist in a given data set?
• Are there proteins or spots that behave similarly to a given protein or spot (i.e. co-regulation)?
• Are there proteins that might be used for the development of noninvasive tests (i.e. diagnostic markers)?
• Are there proteins or protein patterns that might be characteristic of a biological state (e.g. tumor versus normal tissue
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
3. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
4. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
5. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
8. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
9. Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Extended data analysis using DeCyder EDA

(Date:7/22/2014)... , July 22, 2014  United Therapeutics ... that it will release its second quarter 2014 ... 29, 2014. United Therapeutics will host ... at 9:00 a.m. Eastern Time.  The teleconference is ... 1-970-315-0533.  A rebroadcast of the teleconference will be ...
(Date:7/22/2014)... July 22, 2014 Dr. Williams says, ... who have recognized that the greatest health care system ... too often needlessly deadly while trying to serve the ... quasi-experts seeking to transform an enormous system that none ... Williams, in his continuing series of Open Letters ( ...
(Date:7/21/2014)... yield so far is small, but chemists at the ... substitution process to make a precursor to transparent thin ... energy devices. , A paper describing the approach is ... of the journal Inorganic Chemistry , which draws ... nuclear chemistry fields. The paper was chosen by the ...
(Date:7/20/2014)... NY (PRWEB) July 20, 2014 ... custom quartz glass optics, and spectrophotometer calibration ... these years, NSG has released many products that ... successful product has been their solid-state calibration standards. ... liquid standards since their release in 2008. ...
Breaking Biology Technology:Dr. Ira Williams Asks What’s Wrong With Jonathan Bush’s Book, Where Does It Hurt? 2Oregon chemists eye improved thin films with metal substitution 2How NSG Precision Cells Spectrophotometer Calibration Standards Makes NIR Work Easier 2How NSG Precision Cells Spectrophotometer Calibration Standards Makes NIR Work Easier 3
... has been awarded the International Union of Pure and ... Physics. , The award acknowledges Dr Barnard as a ... structures, shapes and crystal structures of materials at the ... Institute of Technology (RMIT) University in 2001 and ...
... Amnis Corporation, a manufacturer of advanced cell imaging ... imaging flow cytometer. The new instrument - the ... the speed, sensitivity, and phenotyping abilities of flow cytometry ... The new ImageStreamX greatly expands the capabilities of ...
... Biotech Ltd. (Amex: SVA ), a leading provider ... a conference call on Friday, May 15, 2009 at 9:00 ... for the period ended March 31, 2009 and provide an ... dial 1-877-407-4018 (USA) or 1-201-689-8471 (international). A replay of ...
Cached Biology Technology:Big prize for 'small science' physicist 2Sinovac to Host Conference Call to Report First Quarter 2009 Financial Results 2
(Date:7/21/2014)... from the strength of immune response, rather than with ... attacking the virus, might be a better way to ... of Washington, Seattle. She and her collaborators have now ... the H7N9 influenza, and their work has already led ... virulent strain. The research is published ahead of print ...
(Date:7/21/2014)... By tracking hybrids between songbird species, investigators have found ... be preventing interbreeding. The research, which is published in ... like GPS, record the position of a bird and ... Compared with their parents, hybrids exhibited increased variability in ... suitable areas, while others used the same routes as ...
(Date:7/21/2014)... apple orchards, effective management practices rely on ... competitive vegetation under the trees, and supplying ... are further complicated in organic management systems ... need to meet the stringent soil fertility ... National Organic Program. University of Arkansas scientists ...
Breaking Biology News(10 mins):Potential new flu drugs target immune response, not virus 2Organic apple orchards benefit from green compost applications 2
... a potent neurotoxin and a worrisome environmental contaminant, but ... what else is in the water. Researchers at ... of dissolved organic material increases the biological risk of ... mercury source. Mercury is present throughout the ...
... & Leuven, Belgium As the most common form ... enormous implications for our ageing society. It is generally ... (A -peptide) protofibrils. Until now, the conditions under which ... the disease remained unknown. VIB researchers have now discovered ...
... will change as carbon dioxide increases, affecting food webs along ... Centre press briefing today. Microbes in the ocean produce ... form above the sea, which reflect the suns rays away ... when they get hot so that clouds will cool them ...
Cached Biology News:Waterborne carbon increases threat of environmental mercury 2Lipids in the brain an important factor for Alzheimer's disease? 2
Complement factor B (human)...
... The AccuSpot automates LC micro-fractionation, ... With the AccuSpot system, LC eluent ... onto target plates, in trace amounts. ... target plates for MALDI-TOF-MS measurements. When ...
... LC Sciences provides a unique new tool, ... synthesis serving diverse needs in genomics, proteomics, ... OligoMix is a MIXTURE of a large ... delivered in a single microtube. OligoMix is ...
... Capillary Electrophoresis Running Buffer was designed to ... demands of todays sequencing labs. As a ... to the gel-based sequencing community Sigma has ... to meet your buffer needs. Scientists at ...
Biology Products: