Navigation Links
Expand Long Template PCR System

Operating parameters
  • Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 68C
Experimental result Amplification of a 29.7 kb and a 9.0 kb factor VIII region from genomic DNA with the Expand Long Template PCR System. Key advantages
  • Increases fidelity of PCR amplification, because the Expand Long Template PCR System enzyme blend combines the proofreading activity of Pwo DNA Polymerase with the high processivity of Taq DNA Polymerase
  • Increases yield of PCR amplification, because the accuracy of the enzyme blend reduces the number of truncated amplification products formed
  • Improves the performance of long PCR, because the buffers included in the Expand Long Template PCR System are optimized for generation of certain length products


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Expand High FidelityPLUS PCR System
2. Expand High Fidelity PCR System
3. Expand 20 kbPLUS PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. High Pure PCR Template Preparation Kit
8. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
9. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
10. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
11. Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Expand Long Template PCR System

(Date:7/10/2014)... WASHINGTON D.C., April 15, 2014 -- According to ... danger to nuclear security comes from terrorists acquiring ... (HEU) to construct a crude nuclear explosive device. ... illicit nuclear trafficking have involved gram-level quantities, which ... methods. , According to a new study appearing ...
(Date:7/10/2014)... has been extremely challenging because there are many genetic ... cause disease. Now investigators reporting in the July 3rd ... Cell describe a strategy that may help reveal ... factors or environmental exposures to affect the development of ... that may predispose individuals to schizophrenia. , The ...
(Date:7/10/2014)... theatergoers have shelled out for crude 3-D glasses, ... viewing experience. These basic devices, used to trick ... may soon be rendered obsolete with the introduction ... University researchers. , TAU doctoral students Yuval Yifat, ... efficient holography based on nanoantennas that could be ...
(Date:1/15/2014)... "The interface is the device," Nobel laureate Herbert ... to be found at the junctures where layers ... of nanotechnology, the interfaces between layers of metal ... such high-tech favorites as spintronics, high-temperature superconductors, ferroelectrics ...
Breaking Biology Technology:Sensitive detection method may help impede illicit nuclear trafficking 2New strategy could uncover genes at the root of psychiatric illnesses 2Projecting a 3-dimensional future 2Projecting a 3-dimensional future 3A deeper look at interfaces 2
... like people are saying nothing in record numbers. , ,In ... using online services such as LiveJournal , Movable Type ... last as long as a New Year's resolution. , ,A ... Corporation suggests that 20 percent of all blogs (short for ...
... The challenges and opportunities that Wisconsin technology companies ... in-depth economic development presentations at the eForum conference ... , a local technology networking association. , ... and technology in Wisconsin's economic future by addressing ...
... shared with you my observations on the French technology market. ... two and a half days talking to technology companies in ... a fascinating experience, as always. , ,Much of the visit ... make about Israel. Let me start by saying that even ...
Cached Biology Technology:Who Gives a Blog? 2High-Tech Economic Development in Southeastern Wisconsin 2High-Tech Economic Development in Southeastern Wisconsin 3The Search for new Global Technologies 2The Search for new Global Technologies 3
(Date:7/11/2014)... that let nerve cells send out electrical signals, Johns ... tactic that may offer a new way to protect ... responsible way. , Their findingthat naturally occurring insect ... for a closely related onesuggests that insecticides can be ... like bees. A summary of the research, led by ...
(Date:7/10/2014)... 30, 2014  Aware, Inc. (NASDAQ: AWRE ... previously announced on June 26, 2014 that its Board ... $1.75 per share, or approximately $40 million in total.  ... 2014 and a payment date of July 24, 2014.  ... had set an ex-dividend date for this special cash ...
(Date:7/10/2014)... July 3, 2014 According to ... Type (Swipe And Area), Material (Optical Prism, Pizeoelectric, Capacitive, ... Security and Banking & Finance), and Geography - Global ... MarketsandMarkets, the global Fingerprint Sensors Market is projected to ... CAGR of 16.8% from 2014 to 2020. ...
Breaking Biology News(10 mins):Potent spider toxin 'electrocutes' German, not American, cockroaches 2Potent spider toxin 'electrocutes' German, not American, cockroaches 3NASDAQ Sets Ex-Dividend Date for Aware, Inc.'s Special Cash Dividend of $1.75 Per Share 2Fingerprint Sensors Market worth $14.35 Billion by 2020 2Fingerprint Sensors Market worth $14.35 Billion by 2020 3
... A (BPA), a chemical used in many polycarbonate plastics ... on development of the prostate gland and brain and ... to a final report released today by the National ... NTP,s current opinion on BPA,s potential to cause harm ...
... release is available in German . Life ... be sustained with the help of a large number of ... knows the phenomenon that a piece of cut apple or ... because the oxygen in the air produces chemical reactions in ...
... man,s height is a modest marker for risk of ... progression of the cancer, say British researchers who conducted ... 58 published studies. In the September issue of ... of the American Association for Cancer Research, 12 researchers ...
Cached Biology News:NTP finalizes report on Bisphenol A 2NTP finalizes report on Bisphenol A 3Oxidative stress: Mechanism of cell death clarified 2Height linked to risk of prostate cancer development and progression 2
Human alpha-1-antitrypsin. No cross-reactivity with alpha-1-acid glycoprotein, serum albumin or other serum proteins....
Phosphorylcholine hapten is conjugated to BSA protein by use of p-diazonium phenylphosphorylcholine....
Viral CMV UL146/vCXC1 Biotinylated Affinity Purified PAb...
Blue Standard is 3-cyano-7-hydroxycoumarin....
Biology Products: