( ...)Expand Long Template PCR System

Operating parameters

• Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
• pH optimum: 9.2
• Divalent ion requirement: Mg²⁺
• Temperature optimum (for elongation): 68C

Experimental result Amplification of a 29.7 kb and a 9.0 kb factor VIII region from genomic DNA with the Expand Long Template PCR System. Key advantages

• Increases fidelity of PCR amplification, because the Expand Long Template PCR System enzyme blend combines the proofreading activity of Pwo DNA Polymerase with the high processivity of Taq DNA Polymerase
• Increases yield of PCR amplification, because the accuracy of the enzyme blend reduces the number of truncated amplification products formed
• Improves the performance of long PCR, because the buffers included in the Expand Long Template PCR System are optimized for generation of certain length products

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