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Expand Long Template PCR System

for amplification of 520 kb targets from eukaryotic genomes Cat. No. 1 681 834 100 units

Cat. No. 1 681 842 2 x 250 units
Cat. No. 1 759 060 10 x 250 units Description Unique enzyme blend of Taq and Pwo DNA Polymerase. The system also includes PCR buffers for optimal amplification of different DNA fragment lengths:
  • PCR buffer 1 (10x conc.), with 17.5 mM MgCl2 (for genomic targets up to 12 kb)
  • PCR buffer 2 (10x conc.), with 22.5 mM MgCl2 (for 1215 kb genomic targets)
  • PCR buffer 3 (10x conc.), with 22.5 mM MgCl2, DMSO, and detergent (for 1520 kb genomic targets and for targets amplified from difficult templates)
Application Produces a high yield of long PCR products from episomal and genomic DNA.
The Expand Long Template PCR System is ideal for amplifying 520 kb fragments from human genomic DNA (or up to 40 kb fragments from DNA). The enzyme blend may be used for:
  • Mapping and sequencing whole genomes
  • Mapping intron/exon structures by amplifying cDNAs
  • Analyzing homologous recombination events
  • Characterizing cloned sequences carried in lambda-phage
  • Amplifying full-length cDNAs or long fragments of cDNA (RACE)
Expand Reverse Transcriptase is a genetically engineered version of M-MuLV Reverse Transcriptase. Point mutation within the RNase H sequence reduces the RNase H activity below detectable levels. This product is not for sale in the United States. Application profile Operating parameters
  • Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 68C
Experimental result Amplification of a 29.7 kb and a 9.0 kb factor VIII region from genomic DNA with the Expand Long Template PCR System. Key advantages
  • Increases fidelity of PCR amplification, because the Expand Long Template PCR System enzyme blend combines the proofreading activity of Pwo DNA Polymerase with the high processivity of Taq DNA Polymerase
  • Increases yield of PCR amplification, because the accuracy of the enzyme blend reduces the number of truncated amplification products formed
  • Improves the performance of long PCR, because the buffers included in the Expand Long Template PCR System are optimized for generation of certain length products



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