Navigation Links
Expand Long Template PCR System

for amplification of 520 kb targets from eukaryotic genomes Cat. No. 1 681 834 100 units

Cat. No. 1 681 842 2 x 250 units
Cat. No. 1 759 060 10 x 250 units Description Unique enzyme blend of Taq and Pwo DNA Polymerase. The system also includes PCR buffers for optimal amplification of different DNA fragment lengths:
  • PCR buffer 1 (10x conc.), with 17.5 mM MgCl2 (for genomic targets up to 12 kb)
  • PCR buffer 2 (10x conc.), with 22.5 mM MgCl2 (for 1215 kb genomic targets)
  • PCR buffer 3 (10x conc.), with 22.5 mM MgCl2, DMSO, and detergent (for 1520 kb genomic targets and for targets amplified from difficult templates)
Application Produces a high yield of long PCR products from episomal and genomic DNA.
The Expand Long Template PCR System is ideal for amplifying 520 kb fragments from human genomic DNA (or up to 40 kb fragments from DNA). The enzyme blend may be used for:
  • Mapping and sequencing whole genomes
  • Mapping intron/exon structures by amplifying cDNAs
  • Analyzing homologous recombination events
  • Characterizing cloned sequences carried in lambda-phage
  • Amplifying full-length cDNAs or long fragments of cDNA (RACE)
Expand Reverse Transcriptase is a genetically engineered version of M-MuLV Reverse Transcriptase. Point mutation within the RNase H sequence reduces the RNase H activity below detectable levels. This product is not for sale in the United States. Application profile Operating parameters
  • Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 68C
Experimental result Amplification of a 29.7 kb and a 9.0 kb factor VIII region from genomic DNA with the Expand Long Template PCR System. Key advantages
  • Increases fidelity of PCR amplification, because the Expand Long Template PCR System enzyme blend combines the proofreading activity of Pwo DNA Polymerase with the high processivity of Taq DNA Polymerase
  • Increases yield of PCR amplification, because the accuracy of the enzyme blend reduces the number of truncated amplification products formed
  • Improves the performance of long PCR, because the buffers included in the Expand Long Template PCR System are optimized for generation of certain length products


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Expand High FidelityPLUS PCR System
2. Expand High Fidelity PCR System
3. Expand 20 kbPLUS PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. TripleMaster and Perfect gDNA Blood Mini Kit Team Up to Amplify Long gDNA Templates
6. Using the TripleMaster PCR System for Robust Amplification of GC-Rich DNA Templates
7. High Pure PCR Template Preparation Kit
8. Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations
9. InstaGene Matrix: Prepare DNA Templates for PCR With No Phenol/Chloroform Extractions and No Deproteinization Steps, Rev C
10. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
11. Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/18/2017)... Malden, Mass. (PRWEB) , ... May 18, 2017 ... ... completed the procedure on April 28, 2017 at the Prince Of Wales Private ... degenerative cervical disc at level C6-C7. The patient failed conservative treatments prior to ...
(Date:5/18/2017)... ... May 17, 2017 , ... Cognition Corporation ( ... just released version 9.0 of the Cognition Cockpit platform. , “Our whole team ... David Cronin, CEO of Cognition. “We’re thrilled to finally be able to release ...
(Date:5/18/2017)... ... May 18, 2017 , ... When James Sherley, was notified earlier this year that ... Brands for the Year 2017 by The Silicon Review , he was not surprised ... progress increasing Asymmetrex’s value, but this recognition by Silicon Valley was particularly meaningful. Our ...
(Date:5/16/2017)... 16, 2017 Telehealth has long been ... something that has been kept completely separate from ... according to   Logicalis Healthcare Solutions , the ... solutions and managed services provider ( www.us.logicalis.com ), ... – interrelationship between telehealth, imaging, and EHR optimization ...
Breaking Biology Technology:
(Date:4/13/2017)... UBM,s Advanced Design and Manufacturing event in ... and evolving technology through its 3D Printing and Smart ... the expo portion of the event and feature a ... on trending topics within 3D printing and smart manufacturing. ... will take place June 13-15, 2017 at the Jacob K. ...
(Date:4/11/2017)... GARDENS, Fla. , April 11, 2017 /PRNewswire/ ... management and secure authentication solutions, today announced that ... by Intelligence Advanced Research Projects Activity (IARPA) to ... IARPA,s Thor program. "Innovation has been ... and IARPA,s Thor program will allow us to ...
(Date:4/11/2017)... N.Y. , April 11, 2017 ... fingerprints, but researchers at the New York University ... College of Engineering have found that partial similarities ... security systems used in mobile phones and other ... thought. The vulnerability lies in the ...
Breaking Biology News(10 mins):