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Expand High FidelityPLUS PCR System

Explore New Horizons in Amplification Yield Combine High Yield, Accuracy, and the Prevention of Carry-Over Contamination Roche Applied Science, which pioneered the blending of thermostable DNA polymerases with the Expand High Fidelity and Expand Long Template PCR Systems, now introduces the Expand High FidelityPLUS PCR System the first product of a family of next-generation PCR Blends. This new blend combines Taq DNA Polymerase with a novel proofreading protein isolated and characterized by Roche Applied Science. This protein mediates proofreading activity, but has no polymerase activity itself. The synergy between the mechanism of the proofreading protein and the processivity of Taq DNA Polymerase is the key to Expand High FidelityPLUS Enzyme Blends unique ability to
  • Deliver higher yields
  • Improve accuracy for high-fidelity applications, and
  • Incorporate dUTP to prevent carry-over contamination
    for amplification reactions up to 5 kb.
Improve the yield and accuracy of your current amplification reaction
  • Obtain higher yields than with any other polymerase or mixture (Figure 1).
  • Benefit from six-fold higher fidelity than with Taq DNA Polymerase alone.
  • Achieve greater sensitivity and specificity than with blends of Taq and proofreading polymerases (Figures 1, 2).
Figure 1: Comparison of yield and accuracy of various thermostable polymerases and blends. Combine the benefits of an enzyme blend with the prevention of carry-over contamination for more reliable results Carry-over contamination, in which the product of a previous PCR erroneously serves as a template in a subsequent reaction, is a problem in every laboratory. Carry-over contamination can be easily prevented by using Taq polymerase to incorporate dUTP, then pretreating all subsequent reactions with Uracil-DNA Glycosylase prior to cycling. However, since blends of Taq polymerase and proofreading polymerases are incapable of incorporating dUTP, researchers have traditionally been forced to choose between Taq polymerases ability to incorporate dUTP and the polymerase blends greater yields and fidelity. Taq polymerase only permitted the amplification of fragments up to ~3 kb, making it impossible to prevent carry-over contamination for longer products. The Expand High FidelityPLUS Enzyme Blend has changed all that. Use it to incorporate dUTP and amplify fragments up to 5 kb with greater yield and better fidelity (Figure 3). Expand High FidelityPLUS PCR System*
Supplied with buffer that contains MgCl2, buffer without
MgCl2, and MgCl2 stock solution Cat. No. Pack Size 3 300 242 125 units for 50 reactions 3 300 226 500 units (2 x 250 units) for 200 reactions 3 300 234 2,500 units (10 x 250 units) for 1,000 reactions Dont forget our PCR-Grade Nucleotides!
For maximum PCR sensitivity, insist on the highest purity
(>99% dNTP, <0.9% dNDP) nucleotides available. Product Cat. No. Pack Size PCR Nucleotide Mix** 1 581 295
1 814 362 100 reactions
1,000 reactions Set of Deoxynucleotides,
PCR Grade** 1 969 064 4 x 25 mol PCR Nucleotide MixPLUS** 1 888 412 2 x 100 l, 200 reactions
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