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Expand High FidelityPLUS PCR System

Explore New Horizons in Amplification Yield Combine High Yield, Accuracy, and the Prevention of Carry-Over Contamination Roche Applied Science, which pioneered the blending of thermostable DNA polymerases with the Expand High Fidelity and Expand Long Template PCR Systems, now introduces the Expand High FidelityPLUS PCR System the first product of a family of next-generation PCR Blends. This new blend combines Taq DNA Polymerase with a novel proofreading protein isolated and characterized by Roche Applied Science. This protein mediates proofreading activity, but has no polymerase activity itself. The synergy between the mechanism of the proofreading protein and the processivity of Taq DNA Polymerase is the key to Expand High FidelityPLUS Enzyme Blends unique ability to
  • Deliver higher yields
  • Improve accuracy for high-fidelity applications, and
  • Incorporate dUTP to prevent carry-over contamination
    for amplification reactions up to 5 kb.
Improve the yield and accuracy of your current amplification reaction
  • Obtain higher yields than with any other polymerase or mixture (Figure 1).
  • Benefit from six-fold higher fidelity than with Taq DNA Polymerase alone.
  • Achieve greater sensitivity and specificity than with blends of Taq and proofreading polymerases (Figures 1, 2).
Figure 1: Comparison of yield and accuracy of various thermostable polymerases and blends.
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