Explore New Horizons in Amplification Yield
Combine High Yield, Accuracy, and the Prevention
of Carry-Over Contamination
Roche Applied Science, which pioneered the blending
of thermostable DNA polymerases with the Expand High Fidelity and Expand
Long Template PCR Systems, now introduces the Expand High FidelityPLUS PCR
System the first product of a family of next-generation PCR Blends.
This new blend combines Taq DNA Polymerase with
a novel proofreading protein isolated and characterized by Roche Applied
Science. This protein mediates proofreading activity, but has no polymerase
The synergy between the mechanism of the proofreading
protein and the processivity of Taq DNA Polymerase is the key to Expand
High FidelityPLUS Enzyme Blends unique ability to
Improve the yield and accuracy of your current
- Deliver higher yields
- Improve accuracy for high-fidelity applications, and
- Incorporate dUTP to prevent carry-over contamination
for amplification reactions up to 5 kb.
Figure 1: Comparison of yield and accuracy
of various thermostable polymerases and blends.
Combine the benefits of an enzyme
blend with the prevention of carry-over contamination for more reliable
- Obtain higher yields than with any other polymerase or
mixture (Figure 1).
- Benefit from six-fold higher fidelity than with Taq DNA Polymerase
- Achieve greater sensitivity and specificity than with blends of Taq
and proofreading polymerases (Figures 1, 2).
Carry-over contamination, in which the product
of a previous PCR erroneously serves as a template in a subsequent reaction,
is a problem in every laboratory. Carry-over contamination can be easily
prevented by using Taq polymerase to incorporate dUTP, then pretreating
all subsequent reactions with Uracil-DNA Glycosylase prior to cycling.
However, since blends of Taq polymerase and
proofreading polymerases are incapable of incorporating dUTP, researchers
have traditionally been forced to choose between Taq polymerases ability
to incorporate dUTP and the polymerase blends greater yields and fidelity.
Taq polymerase only permitted the amplification of fragments up to ~3 kb,
making it impossible to prevent carry-over contamination for longer products.
The Expand High FidelityPLUS Enzyme Blend has
changed all that. Use it to incorporate dUTP and amplify fragments up to
5 kb with greater yield and better fidelity (Figure 3).
Expand High FidelityPLUS PCR System*
Supplied with buffer that contains MgCl2, buffer without
MgCl2, and MgCl2 stock solution
3 300 242
125 units for 50 reactions
3 300 226
500 units (2 x 250 units) for 200 reactions
3 300 234
2,500 units (10 x 250 units) for 1,000 reactions
Dont forget our PCR-Grade Nucleotides!
For maximum PCR sensitivity, insist on the highest purity
(>99% dNTP, <0.9% dNDP) nucleotides available.
PCR Nucleotide Mix**
1 581 295
1 814 362
Set of Deoxynucleotides,
1 969 064
4 x 25 mol
PCR Nucleotide MixPLUS**
1 888 412
2 x 100 l, 200 reactions
Source:Page: All 1 2 3 Related biology technology :1
. Expand High Fidelity PCR System2
. Expand 20 kbPLUS PCR System3
. Expand Long Template PCR System4
. Combine High Yield, Great Accuracy and the
Prevention of Carry-over Contamination by Using
the Novel Expand High FidelityPLUS PCR System5
. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions6
. Assess the In Vivo Activation of Signal Transduction Pathways with
PathDetect Reporting Systems7
. Generate Adenovirus Vectors in E. coli by Homologous Recombination
with the AdEasy Adenoviral Vector System8
. New Yeast Cloning System for Producing Proteins with Native Amino Acid
. Enhanced PCR Cloning System10
. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification11
. Performance Comparisons of Commercial RT-PCR Systems