within the RNase H sequence reduces the RNase H activity below detectable levels. This product is not for sale in the United States.

Application profile Operating parameters

• pH optimum: 9.2
• Divalent ion requirement: Mg²⁺
• Temperature optimum (for elongation): 6872C

Experimental results Figure 1: Expand High Fidelity successfully amplified the fragment from as little as 1 ng of genomic DNA. Figure 2: Consistent amplification was achieved over the full range from 1.5 mM to 5 mM Mg2+ with Expand High Fidelity. Key advantages

• Improves fidelity of PCR amplification, because the enzyme blend amplifies with threefold greater accuracy than Taq DNA Polymerase alone
• Produces more DNA with fewer cycles, because the proofreading activity of the enzyme blend reduces the number of truncated amplification products formed and increases the yield of full-length product
• Saves time, because the enzyme blend requires less buffer optimization than single polymerase amplification systems and is less dependent on magnesium concentration than single polymerase PCR systems
• Enhances results with difficult experimental systems, because the enzyme blend outperforms Taq DNA Polymerase by more accurately amplifying critical sequences (GC-rich sequences or repeat-rich sequences)
• Ideal for multiplex PCR, because
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