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Expand High Fidelity PCR System

within the RNase H sequence reduces the RNase H activity below detectable levels. This product is not for sale in the United States.

Application profile Operating parameters
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 6872C
Experimental results Figure 1: Expand High Fidelity successfully amplified the fragment from as little as 1 ng of genomic DNA. Figure 2: Consistent amplification was achieved over the full range from 1.5 mM to 5 mM Mg2+ with Expand High Fidelity. Key advantages
  • Improves fidelity of PCR amplification, because the enzyme blend amplifies with threefold greater accuracy than Taq DNA Polymerase alone
  • Produces more DNA with fewer cycles, because the proofreading activity of the enzyme blend reduces the number of truncated amplification products formed and increases the yield of full-length product
  • Saves time, because the enzyme blend requires less buffer optimization than single polymerase amplification systems and is less dependent on magnesium concentration than single polymerase PCR systems
  • Enhances results with difficult experimental systems, because the enzyme blend outperforms Taq DNA Polymerase by more accurately amplifying critical sequences (GC-rich sequences or repeat-rich sequences)
  • Ideal for multiplex PCR, because
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