Navigation Links
Expand High Fidelity PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets

Cat. No. 1 732 641 100 units
Cat. No. 1 732 650 500 units
Cat. No. 1 759 078 2500 units Description Unique enzyme blend of Taq and Pwo DNA Polymerases. The Expand High Fidelity PCR System also includes:
  • Expand High Fidelity buffer, 10x conc., with 15 mM MgCl2
  • Expand High Fidelity buffer, 10x conc., without MgCl2
  • MgCl2 stock solution, 25 mM
Application

Expand High Fidelity PCR System is an unique enzyme blend (Taq / Pwo DNA polymerase) that is ideal for amplifying fragments up to 5 kb from human genomic DNA. It gives better results (higher yield and greater fidelity) than Taq DNA Polymerase alone in standard PCR reactions. (See also "How the Expand High Fidelity PCR System Outperforms Taq DNA Polymerase").

Note: If Expand Reverse Transcriptase (Cat. No. 1785826)a, AMV Reverse Transcriptase (Cat. No. 1495062), or the First Strand cDNA Synthesis for RT-PCR (Cat. No. 1483188) is used for cDNA synthesis in a two step RT-PCR, RNA templates with a length up to 5 kb can be amplified. Expand Reverse Transcriptase is a genetically engineered version of M-MuLV Reverse Transcriptase. A point mutation within the RNase H sequence reduces the RNase H activity below detectable levels. This product is not for sale in the United States.

Application profile Operating parameters
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 6872C
Experimental results Figure 1: Expand High Fidelity successfully amplified the fragment from as little as 1 ng of genomic DNA. Figure 2: Consistent amplification was achieved over the full range from 1.5 mM to 5 mM Mg2+ with Expand High Fidelity. Key advantages
  • Improves fidelity of PCR amplification, because the enzyme blend amplifies with threefold greater accuracy than Taq DNA Polymerase alone
  • Produces more DNA with fewer cycles, because the proofreading activity of the enzyme blend reduces the number of truncated amplification products formed and increases the yield of full-length product
  • Saves time, because the enzyme blend requires less buffer optimization than single polymerase amplification systems and is less dependent on magnesium concentration than single polymerase PCR systems
  • Enhances results with difficult experimental systems, because the enzyme blend outperforms Taq DNA Polymerase by more accurately amplifying critical sequences (GC-rich sequences or repeat-rich sequences)
  • Ideal for multiplex PCR, because the enzyme blend produces more homogeneous fragment patterns and greater yields than Taq DNA Polymerase alone


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Expand High FidelityPLUS PCR System
2. Expand 20 kbPLUS PCR System
3. Expand Long Template PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. Comparing Fidelity and Performance of Proofreading PCR Enzymes
6. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
8. High-Fidelity PCR with a Novel Polymerase Mixture
9. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
10. High Fidelity PCR Master
11. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... Houston Methodist Willowbrook Hospital has signed ... to serve as their official health care provider. ... will provide sponsorship support, athletic training services, and ... volunteers, athletes and families. "We are ... and to bring Houston Methodist quality services and ...
(Date:6/23/2016)... FRANCISCO , June 23, 2016   EpiBiome ... has secured $1 million in debt financing from Silicon ... ramp up automation and to advance its drug development ... its new facility. "SVB has been an ... beyond the services a traditional bank would provide," said ...
(Date:6/23/2016)... Apellis Pharmaceuticals, Inc. today announced positive ... its complement C3 inhibitor, APL-2. The trials were ... studies designed to assess the safety, tolerability, pharmacokinetics ... healthy adult volunteers. Forty subjects were ... dose (ranging from 45 to 1,440mg) or repeated ...
(Date:6/23/2016)... ... June 23, 2016 , ... ClinCapture, ... Pennsylvania Convention Center and will showcase its product’s latest features from June 26 ... presenting a scientific poster on Disrupting Clinical Trials in The Cloud during the ...
Breaking Biology Technology:
(Date:4/28/2016)... BANGALORE, India , April 28, 2016 ... of Infosys (NYSE: INFY ), and Samsung SDS, ... partnership that will provide end customers with a more ... payment services.      (Logo: http://photos.prnewswire.com/prnh/20130122/589162 ) ... financial services, but it also plays a fundamental part in ...
(Date:4/15/2016)... 2016  A new partnership announced today will ... decisions in a fraction of the time it ... high-value life insurance policies to consumers without requiring ... Force Diagnostics, rapid testing (A1C, Cotinine and HIV) ... pressure, weight, pulse, BMI, and activity data) available ...
(Date:3/31/2016)... , March 31, 2016  Genomics firm Nabsys ... founding CEO, Barrett Bready , M.D., who returned ... of the original technical leadership team, including Chief Technology ... of Product Development, Steve Nurnberg and Vice President of ... to the company. Dr. Bready served as ...
Breaking Biology News(10 mins):