Navigation Links
Expand High Fidelity PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets

Cat. No. 1 732 641 100 units
Cat. No. 1 732 650 500 units
Cat. No. 1 759 078 2500 units Description Unique enzyme blend of Taq and Pwo DNA Polymerases. The Expand High Fidelity PCR System also includes:
  • Expand High Fidelity buffer, 10x conc., with 15 mM MgCl2
  • Expand High Fidelity buffer, 10x conc., without MgCl2
  • MgCl2 stock solution, 25 mM
Application

Expand High Fidelity PCR System is an unique enzyme blend (Taq / Pwo DNA polymerase) that is ideal for amplifying fragments up to 5 kb from human genomic DNA. It gives better results (higher yield and greater fidelity) than Taq DNA Polymerase alone in standard PCR reactions. (See also "How the Expand High Fidelity PCR System Outperforms Taq DNA Polymerase").

Note: If Expand Reverse Transcriptase (Cat. No. 1785826)a, AMV Reverse Transcriptase (Cat. No. 1495062), or the First Strand cDNA Synthesis for RT-PCR (Cat. No. 1483188) is used for cDNA synthesis in a two step RT-PCR, RNA templates with a length up to 5 kb can be amplified. Expand Reverse Transcriptase is a genetically engineered version of M-MuLV Reverse Transcriptase. A point mutation within the RNase H sequence reduces the RNase H activity below detectable levels. This product is not for sale in the United States.

Application profile Operating parameters
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 6872C
Experimental results Figure 1: Expand High Fidelity successfully amplified the fragment from as little as 1 ng of genomic DNA. Figure 2: Consistent amplification was achieved over the full range from 1.5 mM to 5 mM Mg2+ with Expand High Fidelity. Key advantages
  • Improves fidelity of PCR amplification, because the enzyme blend amplifies with threefold greater accuracy than Taq DNA Polymerase alone
  • Produces more DNA with fewer cycles, because the proofreading activity of the enzyme blend reduces the number of truncated amplification products formed and increases the yield of full-length product
  • Saves time, because the enzyme blend requires less buffer optimization than single polymerase amplification systems and is less dependent on magnesium concentration than single polymerase PCR systems
  • Enhances results with difficult experimental systems, because the enzyme blend outperforms Taq DNA Polymerase by more accurately amplifying critical sequences (GC-rich sequences or repeat-rich sequences)
  • Ideal for multiplex PCR, because the enzyme blend produces more homogeneous fragment patterns and greater yields than Taq DNA Polymerase alone


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Expand High FidelityPLUS PCR System
2. Expand 20 kbPLUS PCR System
3. Expand Long Template PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. Comparing Fidelity and Performance of Proofreading PCR Enzymes
6. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
8. High-Fidelity PCR with a Novel Polymerase Mixture
9. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
10. High Fidelity PCR Master
11. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... 27, 2016  Sequenom, Inc. (NASDAQ: SQNM ... lives through the development of innovative products and services, ... the United States denied its petition to ... of Sequenom,s U.S. Patent No. 6,258,540 (",540 Patent") are ... by the Supreme Court,s Mayo Collaborative Services v. Prometheus ...
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf K. Hoffmann, ... faculty of the University of North Carolina Kenan-Flagler Business School effective ... at UNC Kenan-Flagler, with a focus on the school’s international efforts, leading classes ...
(Date:6/24/2016)... 2016 Epic Sciences unveiled a liquid ... to PARP inhibitors by targeting homologous recombination deficiency ... new test has already been incorporated into numerous ... types. Over 230 clinical trials are ... including PARP, ATM, ATR, DNA-PK and WEE-1. Drugs ...
(Date:6/23/2016)... ... 2016 , ... UAS LifeSciences, one of the leading manufacturers ... Probiotics, into Target stores nationwide. The company, which has been manufacturing high quality ... list of well-respected retailers. This list includes such fine stores as Whole Foods, ...
Breaking Biology Technology:
(Date:4/26/2016)... and LONDON , April 26, ... of EdgeVerve Systems, a product subsidiary of Infosys ... announced a partnership to integrate the Onegini mobile ...      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ) ... customers enhanced security to access and transact across ...
(Date:4/15/2016)... , April 15, 2016 ... the,  "Global Gait Biometrics Market 2016-2020,"  report to ... http://photos.prnewswire.com/prnh/20160330/349511LOGO ) , ,The global gait biometrics ... of 13.98% during the period 2016-2020. ... angles, which can be used to compute factors ...
(Date:3/31/2016)... , March 31, 2016  Genomics firm Nabsys ... founding CEO, Barrett Bready , M.D., who returned ... of the original technical leadership team, including Chief Technology ... of Product Development, Steve Nurnberg and Vice President of ... to the company. Dr. Bready served as ...
Breaking Biology News(10 mins):