( for one-step R...)Expand High Fidelity PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets

Cat. No. 1 732 641 100 units
Cat. No. 1 732 650 500 units
Cat. No. 1 759 078 2500 units Description Unique enzyme blend of Taq and Pwo DNA Polymerases. The Expand High Fidelity PCR System also includes:

• Expand High Fidelity buffer, 10x conc., with 15 mM MgCl₂
• Expand High Fidelity buffer, 10x conc., without MgCl₂
• MgCl₂ stock solution, 25 mM

Application

Expand High Fidelity PCR System is an unique enzyme blend (Taq / Pwo DNA polymerase) that is ideal for amplifying fragments up to 5 kb from human genomic DNA. It gives better results (higher yield and greater fidelity) than Taq DNA Polymerase alone in standard PCR reactions. (See also "How the Expand High Fidelity PCR System Outperforms Taq DNA Polymerase").

Note: If Expand Reverse Transcriptase (Cat. No. 1785826)a, AMV Reverse Transcriptase (Cat. No. 1495062), or the First Strand cDNA Synthesis for RT-PCR (Cat. No. 1483188) is used for cDNA synthesis in a two step RT-PCR, RNA templates with a length up to 5 kb can be amplified. Expand Reverse Transcriptase is a genetically engineered version of M-MuLV Reverse Transcriptase. A point mutation
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