Navigation Links
Expand 20 kbPLUS PCR System

for amplification of long targets (up to 35 kb) from eukaryotic genomes Cat. No. 1 811 002 100 units Description Unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kbPLUS PCR System also includes an optimized reaction buffer, MgCl2 solution, and a human control DNA (template DNA plus beta-globin primers). Application Produces extra long PCR products from genomic DNA. The Expand 20 kbPLUS PCR System is ideal for amplifying >20 kb fragments from human genomic DNA (or up to 47 kb fragments from lambda-DNA). The enzyme blend may be used for any application that requires amplification of very long DNA sequences, such as:
  • Mapping and sequencing whole genomes
  • Cloning and analysis of eukaryotic genes
  • Analyzing disease-causing genes
  • Amplification of full-length viral genomes
  • Analyzing deletions
Application profile Operating parameters
  • Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 68C
Key advantages
  • Ensures the highest fidelity PCR amplification, because the Expand 20 kbPLUS PCR System has an even lower error rate than Expand Long Template PCR System
  • Increases the reliability of long PCR, because the extra thermostable enzyme improves overall performance of the enzyme blend
  • Expands the possible applications of PCR, because the Expand 20 kbPLUS PCR System produces much longer amplification products than other polymerases or polymerase blends
  • Simplifies adaptation of your thermal cycler to long PCR, because the Expand 20 kbPLUS PCR System includes a human control DNA (template DNA and beta-globin human primers) for monitoring cycling conditions


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Expand High FidelityPLUS PCR System
2. Expand High Fidelity PCR System
3. Expand Long Template PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
7. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Enhanced PCR Cloning System
10. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
11. Performance Comparisons of Commercial RT-PCR Systems
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/27/2016)... June 27, 2016  Global demand for enzymes ... through 2020 to $7.2 billion.  This market includes ... cleaning products, biofuel production, animal feed, and other ... and biocatalysts). Food and beverages will remain the ... increasing consumption of products containing enzymes in developing ...
(Date:6/27/2016)... ... June 27, 2016 , ... ... medical technologies, services and solutions to the healthcare market. The company's primary focus ... distribution, manufacturing, sales and marketing strategies that are necessary to help companies efficiently ...
(Date:6/24/2016)... DIEGO , June 24, 2016 ... more sensitively detects cancers susceptible to PARP inhibitors ... circulating tumor cells (CTCs). The new test has ... HRD-targeted therapeutics in multiple cancer types. ... targeting DNA damage response pathways, including PARP, ATM, ...
(Date:6/23/2016)... 23, 2016   Boston Biomedical , an ... designed to target cancer stemness pathways, announced that ... Orphan Drug Designation from the U.S. Food and ... cancer, including gastroesophageal junction (GEJ) cancer. Napabucasin is ... inhibit cancer stemness pathways by targeting STAT3, and ...
Breaking Biology Technology:
(Date:6/2/2016)...   The Weather Company , an IBM Business (NYSE: ... capability in which consumers will be able to interact with ... via voice or text and receive relevant information about the ... Marketers have long sought an advertising solution that can create ... relevant and valuable; and can scale across millions of interactions ...
(Date:5/16/2016)... May 16, 2016   EyeLock LLC , a ... the opening of an IoT Center of Excellence in ... expand the development of embedded iris biometric applications. ... of convenience and security with unmatched biometric accuracy, making ... aside from DNA. EyeLock,s platform uses video technology to ...
(Date:4/28/2016)... First quarter 2016:   , ... the first quarter of 2015 The gross margin was ... 18.8) and the operating margin was 40% (-13) Earnings ... flow from operations was SEK 249.9 M (21.2) , ... SEK 7,000-8,500 M. The operating margin for 2016 is ...
Breaking Biology News(10 mins):