Navigation Links
Expand 20 kbPLUS PCR System

for amplification of long targets (up to 35 kb) from eukaryotic genomes Cat. No. 1 811 002 100 units Description Unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kbPLUS PCR System also includes an optimized reaction buffer, MgCl2 solution, and a human control DNA (template DNA plus beta-globin primers). Application Produces extra long PCR products from genomic DNA. The Expand 20 kbPLUS PCR System is ideal for amplifying >20 kb fragments from human genomic DNA (or up to 47 kb fragments from lambda-DNA). The enzyme blend may be used for any application that requires amplification of very long DNA sequences, such as:
  • Mapping and sequencing whole genomes
  • Cloning and analysis of eukaryotic genes
  • Analyzing disease-causing genes
  • Amplification of full-length viral genomes
  • Analyzing deletions
Application profile Operating parameters
  • Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 68C
Key advantages
  • Ensures the highest fidelity PCR amplification, because the Expand 20 kbPLUS PCR System has an even lower error rate than Expand Long Template PCR System
  • Increases the reliability of long PCR, because the extra thermostable enzyme improves overall performance of the enzyme blend
  • Expands the possible applications of PCR, because the Expand 20 kbPLUS PCR System produces much longer amplification products than other polymerases or polymerase blends
  • Simplifies adaptation of your thermal cycler to long PCR, because the Expand 20 kbPLUS PCR System includes a human control DNA (template DNA and beta-globin human primers) for monitoring cycling conditions


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Expand High FidelityPLUS PCR System
2. Expand High Fidelity PCR System
3. Expand Long Template PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
7. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Enhanced PCR Cloning System
10. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
11. Performance Comparisons of Commercial RT-PCR Systems
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/29/2017)... March 29, 2017  Vermillion, Inc. (NASDAQ: VRML), ... reported on its results for the fourth quarter ... "2016 marked a pivotal year for us ... inclusion, international distribution agreements, major reimbursement progress with ... payer agreements. In addition we cleared our 2 ...
(Date:3/29/2017)... 29, 2017 WuXi Biologics, a leading ... to biologics and a WuXi AppTec Affiliate, today ... Best Bioprocessing Excellence Award from IMAPAC, a leading ... aims to recognize outstanding leaders and trend-setters of ... bioprocessing and biomanufacturing experts in the industry, along ...
(Date:3/29/2017)... LINCOLN, Mass. , March 29, 2017 ... industry-leader in applying mechanistic modeling to drug ... of their collaboration with Zymeworks Inc. for ... drug conjugate therapeutics intended for the treatment ... has supported Zymeworks previously for GLP toxicology ...
(Date:3/29/2017)... ... March 29, 2017 , ... Cancer diagnostics and pathology workflow ... at the Association of Community Cancer Centers (ACCC) 43rd Annual Meeting, CANCERSCAPE ... unites key stakeholders from leading national organizations to share insights on how value-based ...
Breaking Biology Technology:
(Date:3/2/2017)... Summary This report provides all the information ... partnering interests and activities since 2010. Download the ... Deals and Alliance since 2010 report provides an in-depth ... world,s leading life sciences companies. On demand ... of the most up to date deal and company ...
(Date:2/28/2017)... 2017 News solutions for biometrics, bag drop ... ... 14 to 16 March, Materna will present its complete end-to-end ... travel is a real benefit for passengers. To accelerate the ... passenger touch point solutions to take passengers through the complete ...
(Date:2/22/2017)... , Feb. 22, 2017 With the ... ABI Research identifies four technologies that innovative and ... secure significant share in the changing competitive landscape: ... passive authentication.   "Companies can no ... to security," says Dimitrios Pavlakis , Industry ...
Breaking Biology News(10 mins):