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Expand 20 kbPLUS PCR System

for amplification of long targets (up to 35 kb) from eukaryotic genomes Cat. No. 1 811 002 100 units Description Unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kbPLUS PCR System also includes an optimized reaction buffer, MgCl2 solution, and a human control DNA (template DNA plus beta-globin primers). Application Produces extra long PCR products from genomic DNA. The Expand 20 kbPLUS PCR System is ideal for amplifying >20 kb fragments from human genomic DNA (or up to 47 kb fragments from lambda-DNA). The enzyme blend may be used for any application that requires amplification of very long DNA sequences, such as:
  • Mapping and sequencing whole genomes
  • Cloning and analysis of eukaryotic genes
  • Analyzing disease-causing genes
  • Amplification of full-length viral genomes
  • Analyzing deletions
Application profile Operating parameters
  • Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 68C
Key advantages
  • Ensures the highest fidelity PCR amplification, because the Expand 20 kbPLUS PCR System has an even lower error rate than Expand Long Template PCR System
  • Increases the reliability of long PCR, because the extra thermostable enzyme improves overall performance of the enzyme blend
  • Expands the possible applications of PCR, because the Expand 20 kbPLUS PCR System produces much longer amplification products than other polymerases or polymerase blends
  • Simplifies adaptation of your thermal cycler to long PCR, because the Expand 20 kbPLUS PCR System includes a human control DNA (template DNA and beta-globin human primers) for monitoring cycling conditions



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