Navigation Links
Expand 20 kbPLUS PCR System

for amplification of long targets (up to 35 kb) from eukaryotic genomes Cat. No. 1 811 002 100 units Description Unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kbPLUS PCR System also includes an optimized reaction buffer, MgCl2 solution, and a human control DNA (template DNA plus beta-globin primers). Application Produces extra long PCR products from genomic DNA. The Expand 20 kbPLUS PCR System is ideal for amplifying >20 kb fragments from human genomic DNA (or up to 47 kb fragments from lambda-DNA). The enzyme blend may be used for any application that requires amplification of very long DNA sequences, such as:
  • Mapping and sequencing whole genomes
  • Cloning and analysis of eukaryotic genes
  • Analyzing disease-causing genes
  • Amplification of full-length viral genomes
  • Analyzing deletions
Application profile Operating parameters
  • Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
  • pH optimum: 9.2
  • Divalent ion requirement: Mg2+
  • Temperature optimum (for elongation): 68C
Key advantages
  • Ensures the highest fidelity PCR amplification, because the Expand 20 kbPLUS PCR System has an even lower error rate than Expand Long Template PCR System
  • Increases the reliability of long PCR, because the extra thermostable enzyme improves overall performance of the enzyme blend
  • Expands the possible applications of PCR, because the Expand 20 kbPLUS PCR System produces much longer amplification products than other polymerases or polymerase blends
  • Simplifies adaptation of your thermal cycler to long PCR, because the Expand 20 kbPLUS PCR System includes a human control DNA (template DNA and beta-globin human primers) for monitoring cycling conditions


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Expand High FidelityPLUS PCR System
2. Expand High Fidelity PCR System
3. Expand Long Template PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
6. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
7. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
8. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
9. Enhanced PCR Cloning System
10. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
11. Performance Comparisons of Commercial RT-PCR Systems
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/20/2017)... ... ... Diversity focused business accelerator, The Refinery , announced today they are hosting ... technology-driven, women-led startups in Boston, MA, New Haven/Hamden, CT, and Newark, NJ. Fueling ... that week – in Boston, it will be part of the City of Boston's ...
(Date:9/20/2017)... ... September 20, 2017 , ... ... examining the effects of exoskeleton-assisted walking on gait parameters and neuromuscular activity ... "Neuromechanical adaptations during a robotic powered exoskeleton assisted walking session" (doi:10.1080.10790268.2017.1314900) was ...
(Date:9/20/2017)... Aurora, Ohio (PRWEB) , ... September 20, 2017 ... ... automation, systems integration, and building management solutions, announced today the opening of an ... customers in Taiwan and the Greater China region, while developing new relationships in ...
(Date:9/19/2017)... ... September 19, 2017 , ... ... group of funded early-stage tech companies. “Grit” author Angela Duckworth and her team ... the ic@3401 community is Cooley, an international law firm with decades of experience ...
Breaking Biology Technology:
(Date:4/11/2017)... , Apr. 11, 2017 Research and ... Market 2017-2021" report to their offering. ... The global eye tracking market to grow at a ... report, Global Eye Tracking Market 2017-2021, has been prepared based on ... covers the market landscape and its growth prospects over the coming ...
(Date:4/5/2017)... YORK , April 5, 2017 Today ... is announcing that the server component of the HYPR ... known for providing the end-to-end security architecture that empowers ... HYPR has already secured over 15 million ... makers including manufacturers of connected home product suites and ...
(Date:3/30/2017)... HONG KONG , March 30, 2017 ... developed a system for three-dimensional (3D) fingerprint identification by adopting ground ... technology into a new realm of speed and accuracy for use ... applications at an affordable cost. ... ...
Breaking Biology News(10 mins):