( for amplifica...)Expand 20 kbPLUS PCR System

for amplification of long targets (up to 35 kb) from eukaryotic genomes Cat. No. 1 811 002 100 units Description Unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kb^PLUS PCR System also includes an optimized reaction buffer, MgCl₂ solution, and a human control DNA (template DNA plus beta-globin primers). Application Produces extra long PCR products from genomic DNA. The Expand 20 kb^PLUS PCR System is ideal for amplifying >20 kb fragments from human genomic DNA (or up to 47 kb fragments from lambda-DNA). The enzyme blend may be used for any application that requires amplification of very long DNA sequences, such as:

• Mapping and sequencing whole genomes
• Cloning and analysis of eukaryotic genes
• Analyzing disease-causing genes
• Amplification of full-length viral genomes
• Analyzing deletions

Application profile Operating parameters

• Starting sample: up to 500 ng genomic DNA or 10 ng lambda-DNA
• pH optimum: 9.2
• Divalent ion requirement: Mg²⁺
• Temperature optimum (for elongation): 68C

Key advantages

• Ensures the highest fidelity PCR amplification, because the Expand 20 kbPLUS PCR System has an even lower error rate than Expand Long Template PCR System
• Increases the reliability of long PCR, because the extra thermostable enzyme improves overall performance of the enzyme blend
• Expands the possible applications of PCR, because the Expand 20 kb^PLUS PCR System produces much longer amplification products than other polymerases or polymerase blends
• Simplifies adaptation of your thermal cycler to long PCR, because the Expand 20 kb^PLUS PCR System includes a human control DNA (template DNA and beta-globin human primers) for monitoring cycling conditions

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