Navigation Links
Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells


Mammalian
Hamster fibroblast (CHO)
Human B
Human, B lymphomas: BJAB,P3HR-1, B95-8, UC729-6
Human, B-cell, JY, Epstein-Barr virus transformed
Human, C-4I, cervical carcinoma
Human epithelial cells
Human erythroleukemia (K-562)
Human, epithelial, cervix (HeLa)
Human fibroblast
Human, GCT, fibrous histocytoma
Human glioblastoma
Human, HEL cells, eythroleukemia
Human, Hep3b2, hepatocytes
Human, HepG2, hepatoma
Human, HL 60, eythroleukemia
Human hybridoma
Human, JEG-3, choriocarcinoma
Human, K562, chronic myeloid leukemia
Human, kidney, 293
Human, lymphocytes, primary
Human lymphoma EB4, Ly67, HUT-78
Human, MCF-7, breast
Human, MRC-5, lung
Human medullary thyroid (MTC)
Human melanoma (SKMEL 25, SKMEL 28)
Human, pancreatic
Human primary monocytes
Human, Raji, Burkitt lymphoma
Human, red blood cells
Human, skin carcinoma
Human, squamous cell carcinoma, oral and cervical lines
Human, T lymphoma (Jurkat)
Human, T lymphoblastoid, CEMx174
Human, TCCSUP (epithelial-like) bladder carcinoma
Human, U373, glioblastoma
Human, U937, hystiocytic lymphoma
Human, V79, skin cel ls, fibroblasts

Hybrid, mouse/human, A9 fibroblast
Hybrid, rat/mouse, MEL cells

Monkey, kidney (COS-1, COS-7, CV-1, Vero)


Mouse, 3T3, embryo
Mouse, 32d, myeloma
Mouse, B
Mouse, BALB/c 3T3, clone A31, fibroblast, embryo
Mouse, BbSutA, hematopoietic
Mouse, C127, fibroblast, mammary tumor
Mouse, C2, muscle myoblast
Mouse, C2C12, muscle
Mouse, D10.G4.1, T-cell, helper


Mammalian (continued)
Mouse, embryonic stem cells (ES-D3, E14)
Mouse, erythroleukemia cells
Mouse, FDC-PI, Il-3-dependent cell line
Mouse fibroblast
Mouse, hepatoma (Hepa 16)
Mouse, J558-L, myeloma.
Mouse, L-cells
Mouse, L cell derivative, A-9
Mouse, L929, connective tissue
Mouse, LM(TK-), connective tissue
Mouse, mammary epithelial cells
Mouse, myeloma, 32d, p3x63AG8 (X-63)
Mouse macrophage (RAW 264)
Mouse mammary tumor (C127)
Mouse, NIH/3T3; embryo
Mouse, NSO, myeloma cells
Mouse, p3x63AG8; myeloma
Mouse, SP2/0, [Sp-2], myeloma
Mouse T
Mouse, WEHI-3B, myelomonocytic leukemia
Mouse, X-63, myeloma [p3 X63 - Ag8.653]
Ovine (sheep), CSL503, fetal lung
Ovine (sheep), R.E., rumen
Rat-1
Rat brain
Rat, CA7 7, medullary thyroid carcinoma cell line
Rat, D202CC, hepatoma
Rat, fibroblasts
Rat, H4-11-E-C3, hepatoma
Rat, L-6, myoblast
Rat lymphoma (BW5147)
Rat macrophage
Rat myeloma (4B 2/0)
Rat, N62 T cells
Rat, PC12, adrenal pheochromocytoma
Rat, submandibular acini (secretory cells)


Carrot
Chenopodium rubrum
Hedyotis corymbosa
Lactuca sativa (aka chirimen chisha)
Maize
Nicotiana plumbaginofolia; protoplasts from leaf
Oryza sativa, cv. Yamahouci or cv. Nihonbare
Thalictrum rugosum
US/EG Bulletin 2249
Examples of Electro-transfected Eukaryotic
and Prokaryotic Cells


Fungal Cells
Aspergillus (4 species)
Candida (2 species)
Colletotrichum gloeosporioides (a fungal phytopathogen)
Cryptococcus neoformans, ma5 mutants
Dictyostelium discoideum
Fusarium solani
Leptosphaeria maculans
Neurospora crassa
Pichia pastoris
Penicillium urticae
Saccharomyces cerevisiae (6 strains)
Schizosaccharomyces pombe
Trichoderma harzianum


Bacteria, gram negative
Acetobacter xylinium, ATCC 23769
Actinobacillus (2 species)
Agrobacterium (3 species)
Azospirillum brasilense
Bacteroides (3 species)
Bradyrhizobium (2 speci es)
Brucella abortus
Butyrivibrio fibrisolvens
Campylobacter (2 species)
Caulobacter (3 species)
Citrobacter freundii
Cyanobacteria, primarily filamentous, Anabaena species
Enterobacter aerogenes
Escherichia coli (62 strains)
Erwinia (2 species)
Francisella (2 species)
Haemophilus influenzae
Klebsiella (3 species)
Legionella (3 species)
Pasteurella haemolytica
Proteus (2 species)
Pseudomonas (8 species)
Rhizobium (unspeciated)
Rhodopseudomonas viridis
Rhodospirillum molischianum
Rochalimaea quintana
Salmonella (4 species)
Serratia (2 species)
Vibrio (2 species)
Xanthomonas campestris
Yersinia (3 species)


Bacteria, gram positive
Amycolatopsis mediterranei
Amycolatopsis orientalis
Bacillus (7 species)
Brevibacterium (3 species)
Clavibacter (4 species)
Clostridium (2 species)
Corynebacterium (3 species)
Cytophaga johnsonae
Enterococcus (4 species)
Fremyella diplosiphon
Lactobacillus (21 species)
Leuconostoc (3 species)
Listeria (5 species)
Mycobacterium (3 species)
Pediococcus acidilactici
Propionibacterium jensenii
Rhodococcus fascians
Staphylococcus (7 species)
Streptococcus (11 species)


Cyanobacteria
Anabaena
Fremyella diplosip hon
Nostoc
Synechococcus


Other Bacteria
Acholeplasma laidlawii
Methylophillus (3 species)
Mycobacterium (4 species)
Serpula hyodysenteriae
Spiroplasma citri
Treponema hyodysenteriae


Other Cell Types
Anaerobic bacteria
Chicken, HD11, macrophage
Chicken, primary hepatocytes
Chicken, TS34 a6 L1, [LSCC HD2], erythroblast
Chloroplast, spinach
Encapsulated bacteria
Frog, retina
Giardia lamblia
Hydra cells, Cnidaria.
Leishmania, all species within the genus
Marine organisms
Photosynthetic bacteria
Primary chicken oviduct
Sea urchin egg
Tetrahymena
Thermotolerant bacteria
Trypanosoma brucei (blood stream and procyclic forms)


Other Applications
Cotransformation
Cosmids
Carbohydrates
Direct transfer (donor to recipient)
Fluorescent molecules
Influence of DNA size on efficiency
Influence of DNA conformation on efficiency
Large DNA fragments
Ligation mixtures
Library construction
M13 DNA
Plasmid incompatibility
RNA
Release of cell components
Use of more than one electroporation unit


Experimental conditions available as an Electroprotocol


back to top
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
3. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial RNA Isolation From Infected Eukaryotic Hosts
6. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
7. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
8. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
9. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
10. XL10-Gold Cells Supplied in New Single-Reaction Format
11. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/12/2017)... (PRWEB) , ... October 12, 2017 , ... ... partners with the pharmaceutical and biotechnology industries to improve patient outcomes and quality ... Several trends in analytical testing are being attributed to new regulatory requirements for ...
(Date:10/11/2017)... (PRWEB) , ... October 11, 2017 , ... ... any gene in its endogenous context, enabling overexpression experiments and avoiding the use ... with small RNA guides is transformative for performing systematic gain-of-function studies. , ...
(Date:10/11/2017)... ... 11, 2017 , ... Proscia Inc ., a data ... titled, “Pathology is going digital. Is your lab ready?” with Dr. Nicolas Cacciabeve, ... and how Proscia improves lab economics and realizes an increase in diagnostic confidence.* ...
(Date:10/11/2017)... ... 11, 2017 , ... Disappearing forests and increased emissions are the main causes ... each year. Especially those living in larger cities are affected by air pollution related ... the most pollution-affected countries globally - decided to take action. , “I knew I ...
Breaking Biology Technology:
(Date:3/29/2017)...  higi, the health IT company that operates the ... , today announced a Series B investment from ... The new investment and acquisition accelerates higi,s strategy to ... population health activities through the collection and workflow integration ... collects and secures data today on behalf of over ...
(Date:3/27/2017)... , March 27, 2017  Catholic Health Services ... Management Systems Society (HIMSS) Analytics for achieving Stage ... Model sm . In addition, CHS previously earned ... hospitals using an electronic medical record (EMR). ... high level of EMR usage in an outpatient ...
(Date:3/24/2017)... Research and Markets has announced the addition of the "Global ... to 2025" report to their offering. ... The Global Biometric Vehicle Access System Market ... the next decade to reach approximately $1,580 million by 2025. ... for all the given segments on global as well as regional ...
Breaking Biology News(10 mins):