Navigation Links
Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells

Hamster fibroblast (CHO)
Human B
Human, B lymphomas: BJAB,P3HR-1, B95-8, UC729-6
Human, B-cell, JY, Epstein-Barr virus transformed
Human, C-4I, cervical carcinoma
Human epithelial cells
Human erythroleukemia (K-562)
Human, epithelial, cervix (HeLa)
Human fibroblast
Human, GCT, fibrous histocytoma
Human glioblastoma
Human, HEL cells, eythroleukemia
Human, Hep3b2, hepatocytes
Human, HepG2, hepatoma
Human, HL 60, eythroleukemia
Human hybridoma
Human, JEG-3, choriocarcinoma
Human, K562, chronic myeloid leukemia
Human, kidney, 293
Human, lymphocytes, primary
Human lymphoma EB4, Ly67, HUT-78
Human, MCF-7, breast
Human, MRC-5, lung
Human medullary thyroid (MTC)
Human melanoma (SKMEL 25, SKMEL 28)
Human, pancreatic
Human primary monocytes
Human, Raji, Burkitt lymphoma
Human, red blood cells
Human, skin carcinoma
Human, squamous cell carcinoma, oral and cervical lines
Human, T lymphoma (Jurkat)
Human, T lymphoblastoid, CEMx174
Human, TCCSUP (epithelial-like) bladder carcinoma
Human, U373, glioblastoma
Human, U937, hystiocytic lymphoma
Human, V79, skin cel ls, fibroblasts

Hybrid, mouse/human, A9 fibroblast
Hybrid, rat/mouse, MEL cells

Monkey, kidney (COS-1, COS-7, CV-1, Vero)

Mouse, 3T3, embryo
Mouse, 32d, myeloma
Mouse, B
Mouse, BALB/c 3T3, clone A31, fibroblast, embryo
Mouse, BbSutA, hematopoietic
Mouse, C127, fibroblast, mammary tumor
Mouse, C2, muscle myoblast
Mouse, C2C12, muscle
Mouse, D10.G4.1, T-cell, helper

Mammalian (continued)
Mouse, embryonic stem cells (ES-D3, E14)
Mouse, erythroleukemia cells
Mouse, FDC-PI, Il-3-dependent cell line
Mouse fibroblast
Mouse, hepatoma (Hepa 16)
Mouse, J558-L, myeloma.
Mouse, L-cells
Mouse, L cell derivative, A-9
Mouse, L929, connective tissue
Mouse, LM(TK-), connective tissue
Mouse, mammary epithelial cells
Mouse, myeloma, 32d, p3x63AG8 (X-63)
Mouse macrophage (RAW 264)
Mouse mammary tumor (C127)
Mouse, NIH/3T3; embryo
Mouse, NSO, myeloma cells
Mouse, p3x63AG8; myeloma
Mouse, SP2/0, [Sp-2], myeloma
Mouse T
Mouse, WEHI-3B, myelomonocytic leukemia
Mouse, X-63, myeloma [p3 X63 - Ag8.653]
Ovine (sheep), CSL503, fetal lung
Ovine (sheep), R.E., rumen
Rat brain
Rat, CA7 7, medullary thyroid carcinoma cell line
Rat, D202CC, hepatoma
Rat, fibroblasts
Rat, H4-11-E-C3, hepatoma
Rat, L-6, myoblast
Rat lymphoma (BW5147)
Rat macrophage
Rat myeloma (4B 2/0)
Rat, N62 T cells
Rat, PC12, adrenal pheochromocytoma
Rat, submandibular acini (secretory cells)

Chenopodium rubrum
Hedyotis corymbosa
Lactuca sativa (aka chirimen chisha)
Nicotiana plumbaginofolia; protoplasts from leaf
Oryza sativa, cv. Yamahouci or cv. Nihonbare
Thalictrum rugosum
US/EG Bulletin 2249
Examples of Electro-transfected Eukaryotic
and Prokaryotic Cells

Fungal Cells
Aspergillus (4 species)
Candida (2 species)
Colletotrichum gloeosporioides (a fungal phytopathogen)
Cryptococcus neoformans, ma5 mutants
Dictyostelium discoideum
Fusarium solani
Leptosphaeria maculans
Neurospora crassa
Pichia pastoris
Penicillium urticae
Saccharomyces cerevisiae (6 strains)
Schizosaccharomyces pombe
Trichoderma harzianum

Bacteria, gram negative
Acetobacter xylinium, ATCC 23769
Actinobacillus (2 species)
Agrobacterium (3 species)
Azospirillum brasilense
Bacteroides (3 species)
Bradyrhizobium (2 speci es)
Brucella abortus
Butyrivibrio fibrisolvens
Campylobacter (2 species)
Caulobacter (3 species)
Citrobacter freundii
Cyanobacteria, primarily filamentous, Anabaena species
Enterobacter aerogenes
Escherichia coli (62 strains)
Erwinia (2 species)
Francisella (2 species)
Haemophilus influenzae
Klebsiella (3 species)
Legionella (3 species)
Pasteurella haemolytica
Proteus (2 species)
Pseudomonas (8 species)
Rhizobium (unspeciated)
Rhodopseudomonas viridis
Rhodospirillum molischianum
Rochalimaea quintana
Salmonella (4 species)
Serratia (2 species)
Vibrio (2 species)
Xanthomonas campestris
Yersinia (3 species)

Bacteria, gram positive
Amycolatopsis mediterranei
Amycolatopsis orientalis
Bacillus (7 species)
Brevibacterium (3 species)
Clavibacter (4 species)
Clostridium (2 species)
Corynebacterium (3 species)
Cytophaga johnsonae
Enterococcus (4 species)
Fremyella diplosiphon
Lactobacillus (21 species)
Leuconostoc (3 species)
Listeria (5 species)
Mycobacterium (3 species)
Pediococcus acidilactici
Propionibacterium jensenii
Rhodococcus fascians
Staphylococcus (7 species)
Streptococcus (11 species)

Fremyella diplosip hon

Other Bacteria
Acholeplasma laidlawii
Methylophillus (3 species)
Mycobacterium (4 species)
Serpula hyodysenteriae
Spiroplasma citri
Treponema hyodysenteriae

Other Cell Types
Anaerobic bacteria
Chicken, HD11, macrophage
Chicken, primary hepatocytes
Chicken, TS34 a6 L1, [LSCC HD2], erythroblast
Chloroplast, spinach
Encapsulated bacteria
Frog, retina
Giardia lamblia
Hydra cells, Cnidaria.
Leishmania, all species within the genus
Marine organisms
Photosynthetic bacteria
Primary chicken oviduct
Sea urchin egg
Thermotolerant bacteria
Trypanosoma brucei (blood stream and procyclic forms)

Other Applications
Direct transfer (donor to recipient)
Fluorescent molecules
Influence of DNA size on efficiency
Influence of DNA conformation on efficiency
Large DNA fragments
Ligation mixtures
Library construction
Plasmid incompatibility
Release of cell components
Use of more than one electroporation unit

Experimental conditions available as an Electroprotocol

back to top


Page: All 1 2 3 4 5

Related biology technology :

1. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
3. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
4. Bacterial RNA Isolation From Infected Eukaryotic Hosts
5. Bacterial RNA Isolation From Infected Eukaryotic Hosts
6. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
7. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
8. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
9. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
10. XL10-Gold Cells Supplied in New Single-Reaction Format
11. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
Post Your Comments:

(Date:10/8/2015)...  The ALS Association, in partnership with Prize4Life, is ... revolutionize communication technology solutions for people living with ALS. ... (amyotrophic lateral sclerosis) is a progressive neurodegenerative disease that ... spinal cord. Eventually, people with ALS lose the ability ... to total paralysis and death within two to five ...
(Date:10/8/2015)... ... ... Talon Innovations, a provider of Precision Machined Products and Assemblies ... one of three finalists for the Minnesota Business magazine’s Manufacturing Award in the category ... was recognized as a nominee for this competitive award. , The “Best in ...
(Date:10/7/2015)... , Oct. 7, 2015 Boston ... the start of the Boston Scientific Connected Patient ... advancements in the use of remote patient monitoring to ... designers and entrepreneurs can submit their ideas and collaborate ... ( ). Cambridge, MA ...
(Date:10/7/2015)... -- ContraVir Pharmaceuticals, Inc. (Nasdaq: CTRV ) ("ContraVir" or ... and commercialization of targeted antiviral therapies, today announced the ... of its common stock and warrants to purchase up ... a fixed combined price to the public of $3.00. ... issued separately.  The warrants will be exercisable for a ...
Breaking Biology Technology:
... , , HUNTSVILLE, Ala., ... in nucleic acid therapeutics and delivery, announced today that Jason ... Pharmacology to Vice-President of Preclinical Research and Development. , ... management and scientific leadership experience in the biotechnology industry and ...
... , , SAN MARINO, Calif., ... has formed a wholly owned subsidiary called MetaCytoLytics, Inc. This ... of Colorado Professor M. Karen Newell called "metabolic disruption technology" ... generate energy from glucose or from fatty acids. That ...
... , , PASADENA, Calif., Aug. 6 AutoImmune ... $86,000, or $0.01 per share basic and diluted, for the three ... $86,000, or $0.01 per share basic and diluted, for the three ... 30, 2009, the net loss was $256,000, or $0.02 per share ...
Cached Biology Technology:
(Date:9/10/2015)... 10, 2015 Report Details ... Selling Opportunities and Revenue Prospects to Help You ... biologics, especially new drug classes? Get the latest ... with exclusive market data and industry knowledge, benefitting ... results, opportunities and sales predictions. Visiongain,s new ...
(Date:9/9/2015)... , Sept. 9, 2015  NuData Security ... and solution-based milestones, furthering the company,s commitment to ... online fraud. NuData Security,s online fraud ... the company,s growth cycle. The product combines continual ... determine good user behavior from fraudulent behavior, allowing ...
(Date:9/2/2015)... 2015 Security of data and assets ... government organizations, telecommunications, hospitals, as well as individuals. ... being used to prevent unauthorised access. Technological advancements ... individuals could gain access to secure premises after ... hardware devices and numeric codes. However, hackers also ...
Breaking Biology News(10 mins):
... for Medical Research have glimpsed two proteins working together ... nucleus and its exterior compartment, the cytoplasm. The research ... is found in organisms from yeast to humans does ... Investigator Sue Jaspersen, Ph.D., focused on a protein called ...
... at high elevations found in residents of the Tibetan ... related to contemporary Sherpa. These genes were passed on ... mixing, and then amplified by natural selection in the ... by scientists from the University of Chicago and Case ...
... that electrically stimulate the auditory nerve have granted ... people worldwide who otherwise would be totally deaf. Existing ... transmitter about an inch in diameter be affixed to ... joint microphone and power source that looks like an ...
Cached Biology News:
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. ID clarifier: ...
... The GenElute Gel Extraction Kit combines silica- binding ... vacuum column format. DNA fragments of interest are ... solubilizing the gel. The Gel Solubilization Solution can ... in either TBE or TAE buffer. Extracted DNA ...
... are designed for the rapid purification of linear ... gels. The DNA band is excised from an,agarose ... within the base of the column are a ... and impurities back while allowing DNA to selectively ...
... Ultra Agarose Spin Kit is an advanced system for ... purified DNA can be used directly for any downstream ... DNA is ready to use in 56 minutes ... used for TAE and TBE gels No specialised ...
Biology Products: