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Examples of Electro-Transfected Eukaryotic and Prokaryotic Cells


Mammalian
Hamster fibroblast (CHO)
Human B
Human, B lymphomas: BJAB,P3HR-1, B95-8, UC729-6
Human, B-cell, JY, Epstein-Barr virus transformed
Human, C-4I, cervical carcinoma
Human epithelial cells
Human erythroleukemia (K-562)
Human, epithelial, cervix (HeLa)
Human fibroblast
Human, GCT, fibrous histocytoma
Human glioblastoma
Human, HEL cells, eythroleukemia
Human, Hep3b2, hepatocytes
Human, HepG2, hepatoma
Human, HL 60, eythroleukemia
Human hybridoma
Human, JEG-3, choriocarcinoma
Human, K562, chronic myeloid leukemia
Human, kidney, 293
Human, lymphocytes, primary
Human lymphoma EB4, Ly67, HUT-78
Human, MCF-7, breast
Human, MRC-5, lung
Human medullary thyroid (MTC)
Human melanoma (SKMEL 25, SKMEL 28)
Human, pancreatic
Human primary monocytes
Human, Raji, Burkitt lymphoma
Human, red blood cells
Human, skin carcinoma
Human, squamous cell carcinoma, oral and cervical lines
Human, T lymphoma (Jurkat)
Human, T lymphoblastoid, CEMx174
Human, TCCSUP (epithelial-like) bladder carcinoma
Human, U373, glioblastoma
Human, U937, hystiocytic lymphoma
Human, V79, skin cells, fibroblasts

Hybrid, mouse/human, A9 fibroblast
Hybrid, rat/mouse, MEL cells

Monkey, kidney (COS-1, COS-7, CV-1, Vero)


Mouse, 3T3, embryo
Mouse, 32d, myeloma
Mouse, B
Mouse, BALB/c 3T3, clone A31, fibroblast, embryo
Mouse, BbSutA, hematopoietic
Mouse, C127, fibroblast, mammary tumor
Mouse, C2, muscle myoblast
Mouse, C2C12, muscle
Mouse, D10.G4.1, T-cell, helper


Mammalian (continued)
Mouse, embryonic stem cells (ES-D3, E14)
Mouse, erythroleukemia cells
Mouse, FDC-PI, Il-3-dependent cell line
Mouse fibroblast
Mouse, hepatoma (Hepa 16)
Mouse, J558-L, myeloma.
Mouse, L-cells
Mouse, L cell derivative, A-9
Mouse, L929, connective tissue
Mouse, LM(TK-), connective tissue
Mouse, mammary epithelial cells
Mouse, myeloma, 32d, p3x63AG8 (X-63)
Mouse macrophage (RAW 264)
Mouse mammary tumor (C127)
Mouse, NIH/3T3; embryo
Mouse, NSO, myeloma cells
Mouse, p3x63AG8; myeloma
Mouse, SP2/0, [Sp-2], myeloma
Mouse T
Mouse, WEHI-3B, myelomonocytic leukemia
Mouse, X-63, myeloma [p3 X63 - Ag8.653]
Ovine (sheep), CSL503, fetal lung
Ovine (sheep), R.E., rumen
Rat-1
Rat brain
Rat, CA77, medullary thyroid carcinoma cell line
Rat, D202CC, hepatoma
Rat, fibroblasts
Rat, H4-11-E-C3, hepatoma
Rat, L-6, myoblast
Rat lymphoma (BW5147)
Rat macrophage
Rat myeloma (4B 2/0)
Rat, N62 T cells
Rat, PC12, adrenal pheochromocytoma
Rat, submandibular acini (secretory cells)


Carrot
Chenopodium rubrum
Hedyotis corymbosa
Lactuca sativa (aka chirimen chisha)
Maize
Nicotiana plumbaginofolia; protoplasts from leaf
Oryza sativa, cv. Yamahouci or cv. Nihonbare
Thalictrum rugosum
US/EG Bulletin 2249
Examples of Electro-transfected Eukaryotic
and Prokaryotic Cells


Fungal Cells
Aspergillus (4 species)
Candida (2 species)
Colletotrichum gloeosporioides (a fungal phytopathogen)
Cryptococcus neoformans, ma5 mutants
Dictyostelium discoideum
Fusarium solani
Leptosphaeria maculans
Neurospora crassa
Pichia pastoris
Penicillium urticae
Saccharomyces cerevisiae (6 strains)
Schizosaccharomyces pombe
Trichoderma harzianum


Bacteria, gram negative
Acetobacter xylinium, ATCC 23769
Actinobacillus (2 species)
Agrobacterium (3 species)
Azospirillum brasilense
Bacteroides (3 species)
Bradyrhizobium (2 species)
Brucella abortus
Butyrivibrio fibrisolvens
Campylobacter (2 species)
Caulobacter (3 species)
Citrobacter freundii
Cyanobacteria, primarily filamentous, Anabaena species
Enterobacter aerogenes
Escherichia coli (62 strains)
Erwinia (2 species)
Francisella (2 species)
Haemophilus influenzae
Klebsiella (3 species)
Legionella (3 species)
Pasteurella haemolytica
Proteus (2 species)
Pseudomonas (8 species)
Rhizobium (unspeciated)
Rhodopseudomonas viridis
Rhodospirillum molischianum
Rochalimaea quintana
Salmonella (4 species)
Serratia (2 species)
Vibrio (2 species)
Xanthomonas campestris
Yersinia (3 species)


Bacteria, gram positive
Amycolatopsis mediterranei
Amycolatopsis orientalis
Bacillus (7 species)
Brevibacterium (3 species)
Clavibacter (4 species)
Clostridium (2 species)
Corynebacterium (3 species)
Cytophaga johnsonae
Enterococcus (4 species)
Fremyella diplosiphon
Lactobacillus (21 species)
Leuconostoc (3 species)
Listeria (5 species)
Mycobacterium (3 species)
Pediococcus acidilactici
Propionibacterium jensenii
Rhodococcus fascians
Staphylococcus (7 species)
Streptococcus (11 species)


Cyanobacteria
Anabaena
Fremyella diplosiphon
Nostoc
Synechococcus


Other Bacteria
Acholeplasma laidlawii
Methylophillus (3 species)
Mycobacterium (4 species)
Serpula hyodysenteriae
Spiroplasma citri
Treponema hyodysenteriae


Other Cell Types
Anaerobic bacteria
Chicken, HD11, macrophage
Chicken, primary hepatocytes
Chicken, TS34 a6 L1, [LSCC HD2], erythroblast
Chloroplast, spinach
Encapsulated bacteria
Frog, retina
Giardia lamblia
Hydra cells, Cnidaria.
Leishmania, all species within the genus
Marine organisms
Photosynthetic bacteria
Primary chicken oviduct
Sea urchin egg
Tetrahymena
Thermotolerant bacteria
Trypanosoma brucei (blood stream and procyclic forms)


Other Applications
Cotransformation
Cosmids
Carbohydrates
Direct transfer (donor to recipient)
Fluorescent molecules
Influence of DNA size on efficiency
Influence of DNA conformation on efficiency
Large DNA fragments
Ligation mixtures
Library construction
M13 DNA
Plasmid incompatibility
RNA
Release of cell components
Use of more than one electroporation unit


Experimental conditions available as an Electroprotocol


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