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Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

death and thus length of the agonal period.


Table 2. Distribution of RIN values by source of samples

RIN Category Total Number (%) Postmortem Number ( %) Surgical Number (%) Outstanding RIN ≥7 14922 (53.2) 2859 (31.8) 12063 (61.7) Excellent 5 ≥ RIN <7 4717 (16.5) 2074 (23.1) 2643 (13.5) Good RIN <5 8905 (31.2) 4059 (45.1) 4846 (24.8) Total 28544 8992 19552


These results indicate that in order to have 6 samples with high quality RNA from surgical recoveries, one must collect 10 cases. Similarly, 20 cases must be collected from postmortem recoveries to get the same number of the highest quality samples. Clearly, if large numbers of cases are required for a particular study, then careful selection of assay techniques that allow use of samples with a broad range of RIN values will maximize the ease with which sufficient samples can be found to complete the study. Asterand provides frozen tissue samples with a RIN value for the RNA isolated from a portion of the specimen. In this way, we provide our customers with the most information for making informed decisions about experimental methods to employ.


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