Navigation Links
Evaluation of RNA isolated from human tissues: quality control for surgical and postmortem samples

be used for some gene expression profiling techniques if they are designed specifically for samples with degraded RNA. These include PCR using short amplicons of 100 bp or less or the Illumina DASL array system.


Table 1.
RIN numbers by application

RIN Suitability 1-5 PCR assays with short regions of amplification 5.1-6.9 qRT-PCR applications 7.0-10.0 Highly demanding gene array assays


The use of alternative technologies can be important when working with human tissue samples. Human tissues often have degraded RNA at the time of collection. This is in contrast to RNA obtained from tissues collected from young, healthy animals or from cultured cells where it is reasonable to demand that all RNA samples have high integrity with RIN values greater than 8.


Effect of Sample Source on RNA quality
Because Asterand assesses the quality of all frozen tissue samples in our XpressBANK, we have a large database of RNA quality results. We have performed an analysis of the RIN values for 28,544 of these fresh frozen samples, and the data are summarized in Table 2. The samples were from both surgical recoveries, primarily cancer, and postmortem recoveries for normal tissues and chronic diseases that are not usually treated by surgery. Approximately 62% of the surgical samples have RIN values greater than 7; whereas only 32% of the postmortem samples have similar quality. The decreased RNA quality of the postmortem samples is related to the cold ischemia time, and also seems to be related to the cause of
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Evaluation of FlashPlate in a Helicase Assay
2. Accurate Evaluation of the Finnigan Surveyor Autosampler Performance using a Deferred Standard
3. Practical Aspects of Evaluation of Chromatographic Data in Size Exclusion Chromatography
4. Advancing the quality control methodology to assess isolated total RNA and generated fragmented cRNA
5. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
6. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
7. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
8. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
9. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
10. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
11. Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer
Post Your Comments:
(Date:7/30/2015)... ... July 30, 2015 , ... The 2015 Market Research Report ... the current state of the global Propanol market with a focus on the Chinese ... Dow, Carboclor, Mitsui Chemicals, Oxea, ISU Chemical, Sanofi, Royal Dutch Shell, LG Chem and ...
(Date:7/30/2015)... Senomyx , Inc. (NASDAQ: SNMX ), a leading ... and commercialize novel flavor ingredients for the food, beverage, ... the second quarter 2015. "Moving into ... to achieve our commercial and financial goals," stated John ... "Since our last quarterly earnings report, our two partners ...
(Date:7/30/2015)... 2015  Discovery Laboratories, Inc. (Nasdaq: DSCO ... second $1.0M tranche under a previously awarded Phase ... at up to $3.0 million to support continued ... a potential medical countermeasure to mitigate acute and ... an initial $1.0 million under this grant in ...
(Date:7/29/2015)... July 30, 2015 Sanofi, ... publie ses résultats pour le premier semestre 2015. ... les résultats. Visionner l,interview vidéo et ... Au sommaire de l,interview :  ... Moteurs de croissance - Diabète ...
Breaking Biology Technology:Global Propanol Market Technical Data and Manufacturing Analysis 2020 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 3SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 2SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 3SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 4SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 5SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 6SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 7SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 8SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 9Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 2Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 3
... of the Israeli,scientists, in a recent survey on creativity, ... them into a creative state and keep,them in this ... Project Mind Foundation and conducted by TNS Teleseker, the,survey ... was raised,one fifth of the total sample expressed a ...
... AMDL, Inc.,(Amex: ADL ), headquartered in ... Jilin, China, is a vertically integrated specialty,pharmaceutical ... Inc. ("JPI"), AMDL engages in the research, ... AMDL announced,that it has entered into an ...
... 2008 BioSpace, the world,s,leading online bioscience job board ... NC(TM) Career Fair on Thursday, May 1, 2008. The ... 11 am to 4 pm., (Logo: http://www.newscom.com/cgi-bin/prnh/20080222/CLF013LOGO ... Carolina will attend the,biotech job fair to meet in ...
Cached Biology Technology:Project Mind Survey of Israeli Ph.D. Scientists Favors a New Standard of Creativity in Science 2Project Mind Survey of Israeli Ph.D. Scientists Favors a New Standard of Creativity in Science 3AMDL Signs Exclusive Licensing Agreement for Human Papilloma Virus Diagnostic Test Kit in Certain Asian Countries 2AMDL Signs Exclusive Licensing Agreement for Human Papilloma Virus Diagnostic Test Kit in Certain Asian Countries 3Bioscience Career Fair in North Carolina Expected to Attract Well Qualified Job Seekers 2
(Date:7/21/2015)... Passwords have proven futile for securing ... U.S. Office of Personnel Management breach. Biometric authentication ... but developers and end-users are concerned about how ... Biometrics-as-a-Service provider HYPR Corp. announced today the general ... party integration. A video overview ...
(Date:7/13/2015)... , Jul. 13, 2015 NXT-ID, Inc. (NASDAQ: ... biometric authentication company focused on the growing mobile ... provisional patent 62/188684 for BEHAVIORAL-DIRECTED AUTHENTIATION ... new convenient and secure method to make payments.  ... payment methods, introduced with its groundbreaking voice-direct payment ...
(Date:7/9/2015)... , July 9, 2015  Unchained Labs ... announcing its acquisition of Avid Nano. Avid Nano ... scattering (DLS) systems.    Also today, ... fastest and easiest to use protein sizing system. ... measures a protein,s hydrodynamic size, size distribution, aggregation ...
Breaking Biology News(10 mins):HYPR Corp. Launches Industry First Biometric Tokenization Platform 2HYPR Corp. Launches Industry First Biometric Tokenization Platform 3HYPR Corp. Launches Industry First Biometric Tokenization Platform 4NXT-ID Patents Behavior-Directed Payments 2NXT-ID Patents Behavior-Directed Payments 3NXT-ID Patents Behavior-Directed Payments 4Unchained Labs just can't get enough, acquires Avid Nano, rocks out the new pUNk! 2
... be having a negative effect on pinyon pine reproduction, ... same woodland ecosystems, says a University of Colorado Boulder-led ... seed cone production declined by an average of about ... and northwestern Oklahoma over the past four decades, said ...
... in the Journal of Applied Physiology, Nutrition, and ... high fructose corn syrup (HFCS) and sucrose (table sugar) at ... fat in humans, a leading cause of non-alcoholic fatty liver ... body of science that high fructose corn syrup and table ...
... techniques may be increasing the severity of an outbreak ... in Central America and Mexico, according to a University ... current outbreak of coffee rust is the worst seen ... arrived in the region more than 40 years ago. ...
Cached Biology News:Southwest regional warming likely cause of pinyon pine cone decline, says CU study 2Southwest regional warming likely cause of pinyon pine cone decline, says CU study 3New study finds neither HFCS nor table sugar increases liver fat under 'real world' conditions 2Modern growing methods may be culprit of 'coffee rust' fungal outbreak 2Modern growing methods may be culprit of 'coffee rust' fungal outbreak 3
This monoclonal antibody recognizes the light chain of human Factor XII and at 100 nM inhibits 50% of Factor XII activity. It may be used in ELISA to detect human Factor XII and in coagulation assay...
Chicken polyclonal to DCTD ( Abpromise for all tested applications). Antigen: Full length protein (Human) Entrez GeneID: 1635 Swiss Protein ID: P32321...
Mouse monoclonal [SPM325] to Xanthine Oxidase, prediluted ( Abpromise for all tested applications). Antigen: C terminal 358 amino acid fragment of human xanthine oxidase. Entrez Gene ID: 749...
Mouse monoclonal [2G1] to Vancomycin ( Abpromise for all tested applications)....
Biology Products: