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Evaluation of FlashPlate in a Helicase Assay

nuclease activity (Figure 2). Furthermore, the level of DNA unwinding activity was shown to be dependent upon the dosage of T-Antigen added (Figure 3).

FlashPlate allowed the investigator to quantify enzyme activities, and to follow enzyme kinetics in real-time. Similar assays have been performed using other helicases (e.g., HSV helicase - primase) using appropriate assay conditions and nucleic acid substrates. The efficacy of FlashPlate for HTS assays of helicases was demonstrated.


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