• Dedicated removable cassettes for handling and scanning gels—cassettes are available for naked gels or membranes and DALT and SE 600 series gel sandwiches
• Compatibility with Ettan Spot Picker
• Flat field calibration
• Compatible with image analysis software, such as ImageMaster, ImageQuant, or DeCyder software for quantitative evaluation of samples
• Two 1-GB/sec Ethernet connections for data transfer
Principle of operation
Upon excitation by the metal halide lamp, light is emitted from a fluorescently labeled sample in proportion to the amount of labeled compound in the sample. Emitted light is collected and converted to an electronic signal in a CCD. The signal is displayed and analyzed. Data is stored in a 16-bit Figure 3 compares color images of the same 2-D DIGE gel acquired on the Ettan DIGE Imager and the Typhoon™ scanner. For 2-D DIGE gels, the two instruments produce images of similar quality.
TIFF to provide the digital resolution required to characterize subtle signal intensity differences over the wide dynamic range of the instrument.
Figure 2 shows a typical gel image produced with the Ettan DIGE Imager.
Ettan DIGE Imager, including installation kit 63-0056-42
(power cable, CD with scan control
software, test slides, and user manual with
Ettan DIGE Imager Cassette, 276 x 212 mm, 11-0027-04
for DALT gel sandwiches
Ettan DIGE Imager Cassette, 180 x 160 mm, 11-0027-32
for SE 600 series gel sandwich