The Ettan™ DIGE Imager (Fig 1) is a scanning CCD camera designed for applications in the life sciences. In particular, it has been engineered to create high quality images of 2-D DIGE gels. By combining high resolution with precise motion control, the Ettan DIGE Imager produces accurate multichannel images of your Cy™2-, Cy3-, and Cy5-labeled gel. The system has also been designed to image a wide range of other fluorescent gel applications, and can be used with the new ECL Plex™ Western Blotting System.
The imager is controlled from the Ettan DIGE Imager software, and can be set up for a variety of gel formats. Results from the Ettan DIGE Imager are directly compatible with ImageQuant™ TL, ImageMaster™ 2D Platinum, and DeCyder™ 2-D Differential Analysis (DeCyder 2-D) Software.
ImageMaster 2D Platinum v6.0 comes in two flavors: DIGE and non-DIGE. The newly developed DIGE-enabled version of the software comprises powerful and versatile tools for the analysis of images from DIGE experiments. Together with the Ettan DIGE Imager, ImageMaster 2D Platinum v6.0, DeCyder 2-D v6.5, and DeCyder Extended Data Analysis (EDA) Software create a powerful platform of image capture and analysis tools that offers flexibility, versatility, and adaptability for protein profiling.
Ettan DIGE Imager features
• High sensitivity for imaging faint spots
• Software-selectable wavelengths
• Filter bandwidth optimized to minimize crosstalk
• Adjustable exposure level to optimize sensitivity
• Fast scanning for increased throughput
• Full 16 bits/pixel depth for accurate quantitation
• Sealed environment for scanning and protecting wet samples from drying
• Dedicated removable cassettes for handling and scanning gels—cassettes are available for naked gels or membranes and DALT and SE 600 series gel sandwiches
• Compatibility with Ettan Spot Picker
• Flat field calibration
• Compatible with image analysis software, such as ImageMaster, ImageQuant, or DeCyder software for quantitative evaluation of samples
• Two 1-GB/sec Ethernet connections for data transfer
Principle of operation
Upon excitation by the metal halide lamp, light is emitted from a fluorescently labeled sample in proportion to the amount of labeled compound in the sample. Emitted light is collected and converted to an electronic signal in a CCD. The signal is displayed and analyzed. Data is stored in a 16-bit Figure 3 compares color images of the same 2-D DIGE gel acquired on the Ettan DIGE Imager and the Typhoon™ scanner. For 2-D DIGE gels, the two instruments produce images of similar quality.
TIFF to provide the digital resolution required to characterize subtle signal intensity differences over the wide dynamic range of the instrument.
Figure 2 shows a typical gel image produced with the Ettan DIGE Imager.
Ettan DIGE Imager, including installation kit 63-0056-42
(power cable, CD with scan control
software, test slides, and user manual with
Ettan DIGE Imager Cassette, 276 x 212 mm, 11-0027-04
for DALT gel sandwiches
Ettan DIGE Imager Cassette, 180 x 160 mm, 11-0027-32
for SE 600 series gel sandwich es
Ettan DIGE Imager Cassette, with 11-0027-33
low-fluorescent glass, for naked gels
ImageMaster 2D Platinum v6.0 DIGE Enabled 11-0034-25
ImageMaster 2D Platinum v6.0 11-0034-27
DeCyder 2-D Differential Analysis 28-4012-01
Software v6.5, preinstalled network (including
PC and single concurrent network user license)
DeCyder 2-D Differential Analysis 11-0035-82
Software v6.5, one network user license
DeCyder Extended Data Analysis Software, 28-4012-03
one network user license
ImageQuant TL 63-0050-72
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