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Escherichia coli K12

oltage (V) 1,700 V Time constant (T) 5 ms
  • Immediately add 1 ml SOC medium and transfer to a sterile culture tube with a pasteur pipette. Incubate 30-
    60 minutes with moderate shaking at 37 C.
  • Plate on LB plates containing the appropriate selection chemical.
    • Expected Results: Transformation efficiency up to 3.5 x 109 transformants/g of DNA.


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