Navigation Links
Escherichia coli K12

e for 15 minutes and transfer to a prechilled centrifuge bottle. Harvest by centrifugation (20 minutes, 5,000 x g, 2-4 C). Resuspend pellet in 5 ml ice-cold water. Keep the cells cold during the entire procedure. 3. Wash twice with the original culture volume of ice-cold water. Centrifuge as above. Resuspend pellet by swirling in remaining liquid. 4a. If using the cells immediately, place suspension in a prechilled tube and centrifuge (10 minutes, 5,000 x g, 2-4 C). Resuspend the cells in ice-cold water to a final concentration of approximately 2 x 1011 cells/ml. Aliquote 40-300 l cells into prechilled centrifuge tubes. 4b. If freezing the cells for later use, add 40 ml of ice-cold 10% glycerol, mix and centrifuge for 10 minutes, at 5,000 x g and 2-4 C. Resuspend the cells in ice-cold 10% glycerol to a final concentration of approximately, 2 x 1011 cells/ml. Aliquote 40-300 l cells into prechilled centrifuge tubes. quick freeze on dry ice and store at 80 C.

Electroporation of cells:

  1. Add 1 l DNA (10 pg in water) to tubes containing 40 l electrocompetent cells. Homogenize by gently mixing with
    pipette several times.
  2. Transfer mixture to a prechilled cuvette. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O V
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Escherichia coli C600
2. Escherichia coli DH10B
3. Escherichia coli DH5
4. Escherichia coli C600
5. Escherichia coli K12
6. Escherichia coli DH10B
7. Escherichia coli DH5
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Escherichia coli

(Date:7/29/2014)... HOUSTON (July 29, 2014) Tough, ultralight foam ... and shape through a chemical process invented at Rice ... "GO-0.5BN" looks like a nanoscale building, with floors and ... a pair of two-dimensional materials: floors and walls of ... boron nitride platelets. , The researchers say the ...
(Date:7/29/2014)... 29, 2014 Second generation biofuels are a ... fossil fuels. However, as natural gas is very cheap, this ... are not as valuable as they could be in comparison ... financing projects, has made it difficult for renewable energy developers ... off the ground. For these reasons, certain companies have shifted ...
(Date:7/29/2014)... , July 29, 2014 A ... equipment market (types, applications and geography) - Size, Share, ... Opportunities, Segmentation and Forecast, 2013 - 2020", suggests that ... $12.1 billion by 2020 registering a CAGR of 11.1% ... breakthrough achieved by the electro surgical devices in operative ...
(Date:7/29/2014)... Rockville, MD (PRWEB) July 29, 2014 ... (ITI), a vaccine pioneer in the biotechnology industry, ... firm Newport Coast Securities, Inc. to explore financing ... commercializing LAMP-vax technology, a breakthrough vaccine platform with ... immunotherapy market. The company exclusively licensed the well-established ...
Breaking Biology Technology:Tough foam from tiny sheets 2Renewable Waste Intelligence: Could Renewable Chemicals Be the Future for the Renewable Energy Industry? 2Global Surgical Equipment Market is expected to reach $12.1 billion by 2020 - Allied Market Research 2Global Surgical Equipment Market is expected to reach $12.1 billion by 2020 - Allied Market Research 3Immunomic Therapeutics Engages Newport Coast Securities, Inc. 2Immunomic Therapeutics Engages Newport Coast Securities, Inc. 3
... Lab to Offer Quantitative Coronary Angiographic ... Analysis ... plc (NASDAQ: ICLR;,ISIN:IE0005711209), a global provider of outsourced development ... that its Medical Imaging division has become the first ...
... and law enforcement officials may some day have a new ... air for virtually all of the major threat agents that ... is under development by a team of Lawrence Livermore National ... in laboratory and field experiments. , In their latest advance, ...
... San Antonio (June 30, 2008) Researchers at The University ... Emerging Infectious Diseases and The University of Texas Health Science ... believed to play a role in controlling the early infectious ... bioterrorism agent that is the cause of tularemia. The findings ...
Cached Biology Technology:ICON Medical Imaging Launches New Service to Boost Reliability of Cardiology Studies 2Tests show LLNL detection instrument can monitor the air for all major terrorist threat substances 2Tests show LLNL detection instrument can monitor the air for all major terrorist threat substances 3UTSA/UTHSCSA publish results on bio-threat agent 2
(Date:7/30/2014)... journal Scientific Reports , published by Nature ... that while global temperature is indeed increasing, so too ... each year,s average hottest and coldest temperatures will likely ... a wider range of potential high and low temperate ... even as overall temperatures rise, we may still continue ...
(Date:7/29/2014)... New research by academics in the University of Bristol,s ... we take liquid calories into account when planning meals. ... for the Study of Ingestive Behavior Conference (SSIB 2014) ... August], argues that we do. , The team was ... the School of Experimental Psychology. , As part of ...
(Date:7/29/2014)... The advanced optical methods and applications for imaging and ... in the neurosciences were the inspiration behind Neurophotonics ... international society for optics and photonics. , The first ... in the United States, and a special section features ... examples of the impact that optics and photonics will ...
Breaking Biology News(10 mins):Big data confirms climate extremes are here to stay 2SPIE launches Neurophotonics journal with special section on BRAIN Initiative 2SPIE launches Neurophotonics journal with special section on BRAIN Initiative 3
... University of New Hampshire researchers have received a ... farm as a sustainable closed agroecosystem, exploring viable ... grant, from the U.S. Department of Agriculture,s Sustainable ... explore whether closing energy and nutrient cycles could ...
... surface waters in the arid deserts of the U.S. ... fascinated scientists for over two centuries. A new publication ... diversity of closed basins, ancient lake beds, and partially ... Marith C. Reheis of the USGS, "tantalizes scientists with ...
... magazine dedicated to covering the global environment, is launching ... the former editor of Mother Jones and Audubon magazines, ... leading Web sites for commentary and reporting on the ... inaugural edition features articles by noted environmental advocate and ...
Cached Biology News:UNH receives $380,000 grant to study organic dairy as closed ecosystem 2UNH receives $380,000 grant to study organic dairy as closed ecosystem 3Yale Environment 360 magazine launches online 2
... The Label IT siRNA Tracker Intracellular Localization ... directly label and deliver siRNA, of your ... for in vitro tracking experiments. Subcellular localization ... can be monitored following introduction of the ...
... The Label IT siRNA Tracker Intracellular ... provides a straight forward approach to ... your design, in an efficient yet ... experiments. Subcellular localization and functional inhibition ...
... and reproducible immunoprecipitation (IP) by using a 96 ... than regular IPs, which are problematic with regards ... the agarose bed. The binding of the antibody/antigen ... elution of the immune complex can occur with ...
... The Yeast Oligo Microarray Kit (V2), ... to simultaneously characterize expression of many ... gene activity during important biological processes. ... representing 6,256 known ORFs from the ...
Biology Products: